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Sensitive Fluorogenic Derivatization Method for Bile Acids by Dansyl Cadaverine
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.6, pp.429-435
Lee,
Y., Moon,
D., &
Han,
K.
(1998). Sensitive Fluorogenic Derivatization Method for Bile Acids by Dansyl Cadaverine. Analytical Science and Technology, 11(6), 429-435.
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Abstract
Free and glycine conjugated bile acids were detected fluoromatrically in high-performance liquid chromatography after derivatization with dansyl cadaverine. The coupling agent, diethyl phosphorocyanidate was used to form the amide bond between dansyl cadaverine and analytes. The dansyl derivatives of 8 bile acids were separated successfully on Cosmosil ODS column by using linear gradient elution of 20% MeOH-water/<TEX>$CH_3CN$</TEX>. The detection limits of cholic acid was reached 10 pg(S/N
- keywords
- bile acids, dansyl cadaverine, HPLC, fluorescent
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