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Quadruplex Genotype Analysis at HumTH01, HumTPOX, HumCSF1PO and Amelogenin Loci by FoLT-PCR
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.3, pp.260-264
Lee,
Y., Lim,
S., Kang,
P., Choi,
D., Yoon,
S., &
Han,
M.
(1999). Quadruplex Genotype Analysis at HumTH01, HumTPOX, HumCSF1PO and Amelogenin Loci by FoLT-PCR. Analytical Science and Technology, 12(3), 260-264.
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Abstract
A simple and rapid procedure, called FoLT-PCR(Formamide Low Temperature-Polymerase Chain Reaction) was applied to amplifying DNA directly from various forensic biological evidences including human blood, saliva, hair root, or semen without any DNA preparative steps. We added washing step with non-ionic detergent, 1% Triton X-100, and used Taq DNA polymerase instead of Tth DNA polymerase to amplify 3 STR loci and gender allele simultaneouly. Optimal concentration of formamide and annealing temperature were determined empirically to 8%(v/v), and <TEX>$48^{\circ}C$</TEX> respectively. We also compared this method with standard PCR.
- keywords
- FoLT-PCR, DNA amplification, STR, gender identification
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