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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Investigations with respect to the electrochemical properties of carbon paste electrode fabricated using polybutadiene binder

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2007, v.20 no.1, pp.49-54

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Abstract

For the practical use as a biosensor, a rubber electrode bound by polybutadiene was newly devised for the determination of hydrogen peroxide. Then its electrochemical behaviors were investigated. The signal could be obtained at low electrode potential between 0.0 ~ -0.5 V (vs. Ag/AgCl) with a detection limit of <TEX>$1.4{\times}10^{-4}M$</TEX> and its potential dependence was linear in the experimental range. Especially its Lineweaver-Burk plot showed a very good linearity giving the evidence of a good enzyme immobilization on the surface of the electrode. And mechanical stability of the electrode resulted from using rubber binder presented a new possibility for the practical use of biosensor.

keywords
polybutadiene rubber, biosensor, enzyme electrode, hydrogen peroxide, peroxidase


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