- P-ISSN 1225-0163
- E-ISSN 2288-8985
- 권한신청
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
논문 상세
Home > 논문 상세
A fragmentation database of soyasaponins by liquid chromatography with a photodiode array detector and tandem mass spectrometry
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.4, pp.172-179
https://doi.org/10.5806/AST.2021.34.4.172
(Bioresources Science Major, United Graduate School of Agricultural Science, Iwate University)
(Bioresources Science Major, United Graduate School)
(Bioresources Science Major, United Graduate School of Agricultural Science, Iwate University)
(Bioresources Science Major, United Graduate School of Agricultural Science, Iwate University)
(Bioresources Science Major, United Graduate School)
(Bioresources Science Major, United Graduate School of Agricultural Science, Iwate University)
(Bioresources Science Major, United Graduate School of Agricultural Science, Iwate University)
Haereon,
S.
,
Kyosuke,
M.
,
Yohei,
O.
,
&
Chigen,
T.
(2021). . 분석과학, 34(4), 172-179, https://doi.org/10.5806/AST.2021.34.4.172
- 다운로드 수
- 조회수
Abstract
Oleanane-type triterpenoids exist as secondary metabolites in various plants. In particular, soyasaponin, an oleanane-type triterpenoid, is abundant in the hypocotyl of soybean, one of the most widely cultivated crops in the world. Depending on their chemical structure, soyasaponins are categorized as group A saponins or group DDMP (2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one) saponins. The different soyasaponin chemical structures present different health functionalities and taste characteristics. However, conventional phenotype screening of soybean requires a substantial amount of time for functionality of soyasaponins. Therefore, we attempted to use liquid chromatography with a photodiode array detector and tandem mass spectrometry (LCPDA/ MS/MS) for accurately predicting the phenotype and chemical structure of soyasaponins in the hypocotyl of five common soybean natural mutants. In this method, the aglycones (soyasapogenol A [SS-A] and soyasapogenol B [SS-B]) were detected after acid hydrolysis. These results indicated that the base peak and fragmentation differ depending on the chemical structure of soyasaponin with aglycone. Thus, a fragmentation database can help predict the chemical structure of soyasaponins in soyfoods and plants.
- keywords
- triterpenoid saponins, soyasaponins, soybean, LC-PDA/MS/MS
참고문헌
1
1. G. R. Fenwick, K. R. Price, C. Tsukamoto and K. Okubo, ‘In Toxic Substances in Crop Plants’, 1st Ed., p285-327, Woodhead Publishing, United Kingdom, 1991.
2
2. W. A. Oleszek and Z. Bialy, Journal of Chromatography A, 1112, 78-91 (2006).
3
3. P. Krishnamurthy, C. Tsukamoto, Y. Takahashi, Y. Hongo, R. J. Singh, J. D. Lee and G. Chung, Bioscience, Biotechnology, and Biochemistry, 78(12), 1988-1996(2014).
4
4. P. Krishnamurthy, Y. Fujisawa, Y. Takahashi, H. Abe, K. Yamane, K. Mukaiyama, H. R. Son, S. Hiraga, A. Kaga, T. Anai, C. Tsukamoto and M. Ishimoto, Plant Cell Physiol., 60(5), 1082-1097 (2019).
5
5. X. Wang and L. Li, Mass Spectrometry Letters, 11(2), 17-24 (2020).
6
6. M. Shiraiwa, K. Harada and K. Okubo, Agric. Biol. Chem., 55, 323-331 (1991).
7
7. M. Shiraiwa, K. Harada and K. Okubo, Agric. Biol. Chem., 55, 911-917 (1991).
8
8. M. Shiraiwa, F. Yamauchi, K. Harada and K. Okubo, Agric. Biol. Chem., 54, 1347-1352 (1990).
9
9. T. Sayama, E. Ono, K. Takagi, Y. Takada, M. Horikawa, Y. Nakamoto, A. Hirose, H. Sasama, M. Ohashi, H. Hasegawa, T. Terakawa, A. Kikuchi, S. Kato, N. Tatsuzaki, C. Tsukamoto and M. Ishimoto, The Plant Cell, 24, 2123-2138 (2012).
10
10. S. Kudou, M. Tonomura, C. Tsukamoto, M. Shimoyamada, T. Uchida and K. Okubo, Bioscience, Biotechnology, and Biochemistry, 56(1),142-143 (1992).
11
11. W. Zhang, S. P. Teng and D. G. Popovich, J. Agric. Food Chem., 57, 3620-3625 (2009).
권호 내 다른 논문
- Enantioselective electrophoretic behavior of lipoic acid in single and dual cyclodextrin systems
- Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC–UV
- Improved HPLC-UV method for determination of five synthetic dyes in Typha orientalis
- A fragmentation database of soyasaponins by liquid chromatography with a photodiode array detector and tandem mass spectrometry
- Effective determination of nicotine enantiomers from e-liquids and biological fluids by high performance liquid chromatography (HPLC) using dispersive liquid-liquid microextraction (DLLME)
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.