- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Flower MeOH Extract of Panax Notoginseng Attenuates the Production of Nitric Oxide and Pro-inflammatory Cytokines in LPS-stimulated RAW264.7 Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2009, v.30 no.1, pp.150-162
&
(2009). Flower MeOH Extract of Panax Notoginseng Attenuates the Production of Nitric Oxide and Pro-inflammatory Cytokines in LPS-stimulated RAW264.7 Cells. Journal of Korean Medicine, 30(1), 150-162.
- Downloaded
- Viewed
Abstract
Objectives: Inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2) and pro-inflammatory cytokines, TNF-α and IL-1β play a critical role in inflammatory immune response. Therefore, intervention of inflammatory mediator production promises therapeutic benefit for treatment of many chronic inflammatory diseases, such as allergic asthma, rheumatoid arthritis, multiple sclerosis, septic shock and neurodegenerative diseases. In this study, the pharmacological effects of the flower MeOH extract Panax notoginseng (Notoginseng Flos; NF) on inflammation were investigated to address potential therapeutic or toxic effects. Methods: RAW264.7 cells were treated with different concentrations of NF methanol (NF-M) extract in the presence or absence of LPS (1 ㎍/㎖). Results: NF-M extract significantly inhibited LPS-induced production of NO, PGE2 and pro-inflammatory cytokines, TNF-α and IL-1β in a dose-dependent manner. In addition, NF-M extract suppressed mRNA expressions and protein levels of iNOS, COX-2 and pro-inflammatory cytokines in LPS-stimulated RAW264.7 cells. Conclusion: These results indicated that NF-M extract inhibits LPS-induced production of inflammatory mediators in macrophages and demonstrated that NF-M extract possesses anti-inflammatory properties in vitro.
- keywords
- Notoginseng Flos, Panax notoginseng, RAW264.7, nitric oxide, pro-inflammatory cytokines
Reference
1
1 Bosca L, Zeini M, Través PG, Hortelano S. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology. 2005;208(2):249-58.
2
2 Moncada S. Nitric oxide: discovery and impact on clinical medicine. Journal of the Royal Society of Medicine. 1999;92(4):164-9.
3
3 Nathan C. Nitric oxide as a secretory product of mammalian cells. The FASEB journal. 1992;6(12):3051-64.
4
4 Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annual review of medicine. 2002;53:35-57.
5
5 Rocca B, FitzGerald GA. Cyclooxygenases and prostaglandins: shaping up the immune response. International immunopharmacology. 2002;2(5):603-30.
6
6 Andreakos E, Foxwell B, Feldmann M. Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation? Immunological reviews. 2004;202:250-65.
7
7 李時珍, 本草綱目. 北京;人民衛生出版社. 1982:767-8.
8
8 黃宮繡, 本草求眞. 台北;宏業書局有限公司. 1981:236.
9
9 박창국, 이상일, 삼칠근이 TAA 中毒白鼠의 刊損傷에 미치는 影響. 동서의학. 1989;14(1):35-53.
10
10 Wei Y, Fan JM, Pan LP. Effect of Panax notoginseng saponins on human kidney fibroblast. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2002;22(1):47-9. Chinese.
11
11 Park KM, Kim YS, Jeong TC, Joe CO, Shin HJ, Lee YH, Nam KY, Park JD. Nitric oxide is involved in the immunomodulating activities of acidic polysaccharide from Panax ginseng. Planta medica. 2001;67(2):122-6.
12
12 Rhule A, Navarro S, Smith JR, Shepherd DM. Panax notoginseng attenuates LPS-induced proinflammatory mediators in RAW264.7 cells. Journal of ethnopharmacology. 2006;106(1):121-8.
13
13 Wang CZ, Xie JT, Zhang B, Ni M, Fishbein A, Aung HH, Mehendale SR, Du W, He TC, Yuan CS. Chemopreventive effects of Panax notoginseng and its major constituents on SW480 human colorectal cancer cells. International journal of oncology. 2007;31(5):1149-56.
14
14 Xu QF, Fang XL, Chen DF. Pharmacokinetics and bioavailability of ginsenoside Rb1 and Rg1 from Panax notoginseng in rats. Journal of ethnopharmacology. 2003;84(2-3):187-92.
15
15 He W, Zhu Z, Liu J, Ye H, Zeng J, Huang X, Lai F. Study on therapeutic window of opportunity for Panax notoginseng saponins following focal cerebral ischemia/reperfusion injury in rats. Zhong Yao Cai. 2004;27(1):25-7.
16
16 Sun K, Wang CS, Guo J, Horie Y, Fang SP, Wang F, Liu YY, Liu LY, Yang JY, Fan JY, Han JY. Protective effects of ginsenoside Rb1, ginsenoside Rg1, and notoginsenoside R1 on lipopolysaccharide-induced microcirculatory disturbance in rat mesentery. Life sciences. 2007; 81(6):509-18.
17
17 Gao X, Dan M, Zhao A, Xie G, Jia W. Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography. Biomedical chromatography. 2008;22(3):244-9.
18
18 Wang CZ, Luo X, Zhang B, Song WX, Ni M, Mehendale S, Xie JT, Aung HH, He TC, Yuan CS. Notoginseng enhances anti-cancer effect of 5-fluorouracil on human colorectal cancer cells. Cancer chemotherapy and pharmacology. 2007;60(1):69-79.
19
19 Abreu MT, Arditi M. Innate immunity and toll-like receptors: clinical implications of basic science research. Jornal de pediatria. 2004;144(4):421-9.
20
20 Hanada T, Yoshimura A. Regulation of cytokine signaling and inflammation. Cytokine & growth factor reviews. 2002;3(4-5):413-21.
21
21 張錫純. 醫學衷中參書錄. 서울:醫聖堂:1999:77.
22
22 한의과대학 본초학 편찬위원회. 본초학. 서울:영림사. 2007:440-1.
23
23 Chang SH, Choi Y, Park JA, Jung DS, Shin J, Yang JH, Ko SY, Kim SW, Kim JK. Anti-inflammatory effects of BT-201, an n-butanol extract of Panax notoginseng, observed in vitro and in a collagen-induced arthritis model. Clinical nutrition. 2007;26(6):785-91.
24
24 Assinewe VA, Amason JT, Aubry A, Mullin J, Lemaire I. Extractable polysaccharides of Panax quinquefolius L. (North American ginseng) root stimulate TNF-alpha production by alveolar macrophages. Phytomedicine. 2002;9(5):398-404.
25
25 Jin UH, Park SG, Suh SJ, Kim JK, Kim DS, Moon SK, Lee YC, Park WH, Kim CH. Inhibitory effect of Panax notoginseng on nitric oxide synthase, cyclo-oxygenase-2 and neutrophil functions. Phytotherapy research. 2007;21(2):142-8.
26
26 Park WH, Lee SK, Kim CH. A Korean herbal medicine, Panax notoginseng, prevents liver fibrosis and hepatic microvascular dysfunction in rats. Life Sciences. 2005;76(15):1675-90.
27
27 Yang ZG, Sun HX, Ye YP. Ginsenoside Rd from Panax notoginseng is cytotoxic towards HeLa cancer cells and induces apoptosis. Chemistry & biodiversity. 2006;3(2):187-97.
28
28 Li L, Sheng Y, Zhang J, Guo D. Determination of four active saponins of Panax notoginseng in rat feces by high-performance liquid chromatography. Journal of chromatographic science. 2005;43(8):421-5.
29
29 Wan JB, Yang FQ, Li SP, Wang YT, Cui XM. Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD. Journal of pharmaceutical and biomedical analysis. 2006;41(5):1596-601.
30
30 Gao H, Wang F, Lien EJ, Trousdale MD. Immunostimulating polysaccharides from Panax notoginseng. Pharmaceutical research. 1996;13(8):1196-200.
31
31 Ng TB. Pharmacological activity of sanchi ginseng (Panax notoginseng). The Journal of pharmacy and pharmacology. 2006;58:1007-19.
32
32 Guha M, Mackman N. LPS induction of gene expression in human monocytes. Cellular Signaling. 2001;13:85-94.
33
33 Raabe T, Bukrinsky M, Currie RA. Relative contribution of transcription and translation to the induction of tumor necrosis factor-alpha by lipopolysaccharide. The Journal of biological chemistry. 1998;273:974-80.
34
34 Komakine N, Okasaka M, Takaishi Y, kawazoe K, Murakami K, Yamada Y. New Dammarane-Type Saponin from Roots of Panax notoginseng. Natural Medicines. 2006;60:135-7.
35
35 MJ Cho, SY Lee, JS Kim, JH Lee, HS Choi, HY Lee, HK Ha, CS Kim, SS Kang. Isolation of a cerebroside from Panax notoginseng. The Journal of Korean Society of Pharmacognogy. 2006;37(2):81-4.
36
36 WR Lee, BY Pyun. Serum Level of TNF-alpha and Soluble TNF Receptor I in Infants with RDS and Their Significance as a Prospective Indicator for Development of Infantile Asthma. The Journal of Korean Academy of Pediatric Allergy and Respiratory Disease. 1999;9(3):280-9.
37
37 Lampinen M, Carlson M, Håkansson LD, Venge P. Cytokine-regulated accumulation of eosinophils in inflammatory disease. Allergy. 2004;59(8):793-805.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Study on Standard Herb Prescriptions Based on the Specific Names of the Sick and Wounded for the Application of Oriental Herbal Medicine Health Insurance
- Effect of the Combination of Total Saponin of Red Ginseng and Coisis Semen for the Prevention and Treatment of Obesity
- A Study on the Correlation between Pulse Energy and Sasang Constitutional Syndromes
- A Development Study of Common Clinical Document Forms for Traditional Korean Medicine Information Standardization
- Effects of Sotosaja-hwan on the Generation of ROS, RNS, and on the Expression of NF-κB-dependent Proteins in ob/ob Mouse
- Effects of Samultang on Glutamate-Induced Apoptosis of Hippocampus Cells
- A Clinical Study on the Repeatability and Reproducibility of Ryodoraku Score
- The Effect of Stress During Pregnancy on the Skin Barrier of Mice Four Weeks After Birth
- A Comparative Study about Cerebrovascular Reactivity from a Single Medication and Continuous Medication on Healthy Subjects
- The Relation of Dampness-Phlegm and Metabolic Syndrome in Acute Stroke Patients
- The Effect of Sahyangsohapwon on Heart Rate Variability of Healthy Subjects
- Study on 110 cancer patients treated by combination of Oriental and conventional treatment
- A Review of the Study on Complementary and Alternative Medicine in Obstetrics
- Flower MeOH Extract of Panax Notoginseng Attenuates the Production of Nitric Oxide and Pro-inflammatory Cytokines in LPS-stimulated RAW264.7 Cells
- Report of Two Cases of Shoulder Pain Diagnosed through MRI as Partial Tear of Supraspinatus Tendon Treated by Oriental Medical Treatment
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.