- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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The Effect of Rhus verniciflua Stokes Extract on Apoptosis and Autophagy in human leukemic cell line (MOLT-4)
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2024, v.45 no.3, pp.154-167
Juyoung,
L.
, Jang-Hoon,
L.
, &
Youngchul,
K.
(2024). The Effect of Rhus verniciflua Stokes Extract on Apoptosis and Autophagy in human leukemic cell line (MOLT-4). Journal of Korean Medicine, 45(3), 154-167.
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Abstract
Objectives: This study was performed to investigate the effects of Rhus verniciflua Stokes (RVS) on apoptosis and autophagy in the human leukemic cell line, MOLT-4. Methods: Cell viability was measured by MTS/PMS assay, and cell cycle distribution was analyzed by flow cytometry. The expression levels of mRNA implicated in apoptosis and ER-stress were investigated with RT-qPCR. Lastly, apoptosis- and autophagy-related protein expressions were measured by Western blot analysis. Results: RVS inhibited proliferation of MOLT-4 in a dose-dependent manner over 24, 48 and 72 hours. RVS treatment also induced an increase in subG1 phase. Exposure to RVS increased the expression of the mRNAs encoding Bax and caspase-3, while decreasing the expression of Bcl-2 mRNA, suggesting that RVS induced apoptosis in MOLT-4 cells. Additionally, RVS extract up-regulated ER-stress related mRNAs such as IRE1α, CHOP, PERK and ATF6. Changes in RVS extract-induced apoptosis and autophagy proteins on MOLT-4 cells were also investigated. The level of Bcl-2 was decreased, whereas the levels of Bax, caspase-3, AMPK, Beclin-1, Atg5, p62, and LC3II were increased. Conclusion: These results suggest that RVS would be beneficial in the treatment of Acute Lymphoblastic Leukemia.
- keywords
- Rhus verniciflua Stokes, Rhois Vernicifluae Cortex, MOLT-4, Acute Lymphoblastic Leukemia, Apoptosis, Autophagy
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