ISSN : 1225-3480
본 연구를 통해 지중해담치 (Mytilus galloprovincialis) 의 배아를 이용한 생물검정의 최적 실험 조건을 결정하였다. 결정되어진 조건은 다음과 같다.생물검정 시 노출배양의 온도는 15℃가 가장 적합하였으며, 이 온도에서의 48 시간 노출 이후 실험생물이 피면자(veliger) 유생단계까지 발생하는 시간이었다.배양 가능한 시료의 염분 범위는 30-35 psu 이다. 검정생물 발생의 최적 염분범위이므로 생물검정 시 시료의 염분이 이 조건 범위를 벗어날 경우 시료의 염분을 보정할 필요가 있었다.배양 시 발생 도중 일어날 수 있는 밀도 종속적 효과가 나타나지 않는 최대의 밀도는 100 embryos/ml 이었다.
The embryos of marine bivalves have been commonly used in bioassays for the quality assessment of marine environments. Although several standard protocols for developmental bioassay with bivalves have been already proposed, there have been few trials for applying these protocols in environmental assessment, or for developing new protocol with Korean species. So, there is a strong need to establish the standard bioassay protocols using bivalves commonly found in Korean waters. Prior to developing a new protocol, it is essential to know the optimum conditions for the reliable bioassay procedures. Here, we established the purpose of this study to determine the optimum bioassay conditions for successful development of a common mussel, Mytilus galloprovincialis. The conditions considered as critical for developmental bioassay, and determined in this study were; (1) temperature, (2) salinity, and (3) initial density of embryo. The optimal temperature for developmental bioassay of M. galloprovincialis was determined as 15℃. At this temperature, the required time for the embryo to become veliger larva was 48 hr. The acceptable range of salinity for the embryotoxicity test using M. galloprivincialis was from 30 to 35 psu, which was narrower than that of the natural habitat of adult populations. The optimum density of embryo at the beginning of bioassay was 100 embryos/ml. Over this density, the proportion of normally developed larvae decreased significantly. The results obtained in this study will serve as a basis for preparation of the standard bioassay protocol using embryo of M. galloprovincialis.
Philadelphia, (1989) Standard Guide for Conducting Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs American Society for Testing and Materials,
Philadelphia, (1994) Standard Guide for Conducting Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs American Society for Testing and Materials,
Beiras, R.,, (2003a) Assessment of costal marine pollution in Galicia metal concentration in seawater, Mytilus galloprovincialis
Beiras, R.,, (2003b) Integrative assessment of marine pollution in Galician estuaries using sediment chemistry,
Beiras, R. , (2004) Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals Implication for the implementation of seawater quality criteria,
Choe, B.L.,, (1999) Commercial Molluscs from the Freshwater and Continental Shelf in Korea, Kudeok Press
Geffard, O.,, (2001) Qualit biologique de l'eau de mervalue in situ par le test embryo-larvaire de Crassostrea gigas et Mytilus galloprovincialis,
Goldberg, E.D, (2000) Beyond the mussel watch - new directions for monitoring marine pollution,
His, E., Seaman, (1996) A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment,
Hur, Y.B. , (2000) Development and growth of larvae of four bivalve species Journal of Aquaculture,
Jha, A.N.,, (2000) Detection of genotoxins in the marine environment adoption and evaluation of an integrated approach using the embryolarval stages of the marine mussel,
Kurt, P.B, (2004) A survey to determine levels of chlorinated pesticides and PCBs in mussels and seawater from the Mid-Black Sea Coast of Turkey,
Nicholson, S. , (2003) Accumulation of metal in the soft tissues byssus and shall of the mytilid mussel Perna viridis from polluted and uncontaminated locations in Hong Kong coastal waters,
Radenac, G, (2001) Bioaccumulation and toxicity of four dissolved metals in Paracentrotus lividus sea-urchin embryo,
Smolders, R, (2004) In situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems,
Wedderburn, J, (2000) The field application of cellular and physiological biomarkers in conjunction with early life stage bioassays and adult histopathology,
