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ISSN : 1225-3480
본 연구를 통해 지중해담치 (Mytilus galloprovincialis) 의 배아를 이용한 생물검정의 최적 실험 조건을 결정하였다. 결정되어진 조건은 다음과 같다.생물검정 시 노출배양의 온도는 15℃가 가장 적합하였으며, 이 온도에서의 48 시간 노출 이후 실험생물이 피면자(veliger) 유생단계까지 발생하는 시간이었다.배양 가능한 시료의 염분 범위는 30-35 psu 이다. 검정생물 발생의 최적 염분범위이므로 생물검정 시 시료의 염분이 이 조건 범위를 벗어날 경우 시료의 염분을 보정할 필요가 있었다.배양 시 발생 도중 일어날 수 있는 밀도 종속적 효과가 나타나지 않는 최대의 밀도는 100 embryos/ml 이었다.
The embryos of marine bivalves have been commonly used in bioassays for the quality assessment of marine environments. Although several standard protocols for developmental bioassay with bivalves have been already proposed, there have been few trials for applying these protocols in environmental assessment, or for developing new protocol with Korean species. So, there is a strong need to establish the standard bioassay protocols using bivalves commonly found in Korean waters. Prior to developing a new protocol, it is essential to know the optimum conditions for the reliable bioassay procedures. Here, we established the purpose of this study to determine the optimum bioassay conditions for successful development of a common mussel, Mytilus galloprovincialis. The conditions considered as critical for developmental bioassay, and determined in this study were; (1) temperature, (2) salinity, and (3) initial density of embryo. The optimal temperature for developmental bioassay of M. galloprovincialis was determined as 15℃. At this temperature, the required time for the embryo to become veliger larva was 48 hr. The acceptable range of salinity for the embryotoxicity test using M. galloprivincialis was from 30 to 35 psu, which was narrower than that of the natural habitat of adult populations. The optimum density of embryo at the beginning of bioassay was 100 embryos/ml. Over this density, the proportion of normally developed larvae decreased significantly. The results obtained in this study will serve as a basis for preparation of the standard bioassay protocol using embryo of M. galloprovincialis.
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