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ISSN : 1225-3480
본 연구는 백합과 이매패류인 바지락, Ruditapes philippinarum을 노닐페놀에 노출시켰을 때, 생식 및 조직학적 지표 변화에 대해서 조사하였다. 실험기간은 16주였다. 실험구는 1개의 대조구와 3개의 노닐페놀 노출구인 0.05, 0.1,0.2 mg NP L^(-1)였다. 생식소지수는 암컷의 경우, 대조구보다노출구에서 더 높았으며, 수컷의 경우, 대조구보다 노출구에서더 낮았다. 이성생식세포 발현율은 14.56%였으며, 수컷에서의발현율이 더 높았다. 기관계 가운데 외투막에서 혈림프동의 확장, 내부상피층 자유면에 발달하는 선조연의 탈락과 박리, 점액세포의 변성이 관찰되었다. 아가미에서는 정단섬모와 측면섬모의 부분적 소실, interlamellar junction 붕괴, 점액세포의 변성이 확인되었다. 발에서는 상피세포의 증식, 선조연의소실 및 섬모가 탈락되었다. 지방갈색소 축적은 노닐페놀에 농도의존적으로 높았다. 본 연구결과 노닐페놀은 바지락의 낮은생존율과 조직병리학적 변화 및 이성생식세포 발현를 유도하며, 더욱이 생식과 관련하여 estrogenic effector로써 가능성을 확인하였다.
This study was investigated to find out the change of reproduction and histological biomarker of Ruditapes philippinarum exposed to nonylphenol (NP). Experimental period was 16 weeks. Experimental groups were composed of one control condition and three NP exposure conditions (0.05, 0.1, 0.2 mg NP L^(-1). Gonad index was higher in exposure group than control in female and higher in control than exposure group in male. Intersexuality in the NP exposure group was 14.56% with male exhibiting higher ratio than female. Histological analysis of organ system illustrated expansion of hemolymph sinus, loss of striated border of inner epidermis and degeneration of mucous cell in the mantle. In the gill partially disappearance of frontal and lateral cilia, degeneration of mucous cell were observed. Also, hyperplasia of epithelium and disappearance of striated border were observed in the foot. As the concentration of NP increased, the accumulation of lipofuscin increased in the digestive gland. The results of this study suggest that NP induces reduction of survival rate, histopathology and intersex of R. philippinarum. Furthermore, it is indentified that NP functions as an estrogenic effector on the reproduction of R. philippinarum.
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