ISSN : 1225-3480
채묘시험은 4구간의 수온구 20.0, 23.0, 26.0, <TEX>$29.0^{\circ}C$</TEX>에 염분을 20.0, 30.0, 40.0으로 조절하여 온도와 염분의 혼합구에 대한 참굴 부착기 유생의 채묘시험을 실시한 결과, 수온 <TEX>$29.0^{\circ}C$</TEX>에서 염분 20.0과 30.0으로 조절된 채묘구에서 평균 <TEX>$43.1{\pm}0.19%$</TEX>와 <TEX>$42.1{\pm}0.1%$</TEX>로 유의적으로 가장 높은 채묘율을 보였고, 수온 <TEX>$23.0^{\circ}C$</TEX>염분 40.0에서 <TEX>$34.9{\pm}0.6%$</TEX> 가장 낮은 부착률을 보였다 (P < 0.05). 부착밀도는 수온 <TEX>$29.0^{\circ}C$</TEX>에서 유의적으로 높았고, 염분 40.0의 고염분에서 유의적으로 낮았다(P < 0.05). 그러나 수온과 염분의 혼합효과는 유의적인 차이가 없었다 (P > 0.05). 수온에 따른 염분별 채묘구의 부착 빈도를 조사한 결과 가장 많은 부착빈도는 1.2-2.3 마리/<TEX>$cm^2$</TEX>을 보였고, 평균 부착밀도는 1.9-2.4마리/<TEX>$cm^2$</TEX>였다. 전체적으로 채묘율과 평균 부착밀도 그리고 부착빈도를 감안하면 본 실험 결과 효과적인 채묘관리를 위한 수온과 염분조건은 수온 <TEX>$29.0^{\circ}C$</TEX>, 염분 20.0-30.0으로 조절하는 것이 좋을 것으로 판단된다.
채묘시험은 4구간의 수온구 20.0, 23.0, 26.0, 29.0℃에 염분을 20.0, 30.0, 40.0으로 조절하여 온도와 염분의 혼합구에대한 참굴 부착기 유생의 채묘시험을 실시한 결과, 수온 29. 0℃에서 염분 20.0과 30.0으로 조절된 채묘구에서 평균 43.1± 0.19%와 42.1 ± 0.1%로 유의적으로 가장 높은 채묘율을보였고, 수온 23.0℃염분 40.0에서 34.9 ± 0.6% 가장 낮은부착률을 보였다 (P ＜ 0.05). 부착밀도는 수온 29.0℃에서유의적으로 높았고, 염분 40.0의 고염분에서 유의적으로 낮았다(P ＜ 0.05). 그러나 수온과 염분의 혼합효과는 유의적인 차이가 없었다 (P > 0.05). 수온에 따른 염분별 채묘구의 부착빈도를 조사한 결과 가장 많은 부착빈도는 1.2-2.3 마리/cm2 을 보였고, 평균 부착밀도는 1.9-2.4마리/cm2였다. 전체적으로채묘율과 평균 부착밀도 그리고 부착빈도를 감안하면 본 실험결과 효과적인 채묘관리를 위한 수온과 염분조건은 수온 29. 0℃, 염분 20.0-30.0으로 조절하는 것이 좋을 것으로 판단된다.
Andrews, W.R., Targett, N.M. and Epifanio, C.E. (2001) Isolation and characterization of the metamorphic inducer of the common mud crab, Panopeus herbstii. Journal of Experimental Marine Biology and Ecology, 261: 121–134.
Angell, C.L. (1986) The Biology and Culture of Tropical Oysters. ICLARM Technical Report, No. 13: 42. Manila, Philippines.
Bonar, D.B., Coon, S.L., Walch, M., Weiner, R.M. and Fitt, W. (1990) Control of oyster settlement and metamorphosis by endogenous and exogenous chemical cues. Bulletin of marine Science, 46: 484–498.
Cann-Moisan, C., Nicolas, L. and Robert, R. (2002) Ontogenic changes in the contents of dopamine, norepinephrine and serotonin in larvae and postlarvae of the bivalve Pecten maximus. Aquatic Living Resources, 15: 313–318.
Coon, S.L., Bonar, D.B. and Weiner, R.M. (1985) Induction of settlement and metamorphosis of the Pacific oyster, Crassostrea gigas (Thunberg), by L-DOPA and catecholamines. Journal of Experimental Marine Biology and Ecology, 94: 211–221
Crisp, D.J. (1984) Overview of research on marine invertebrate larvae. In: Grant, P.T. and Mackie, A.M. Eds., Chemoreception in Marine Organisms, vol. 1. 177–265. Academic Press, London.
Devakie, M.N. and Ali, A.B. (2000) Salinity–temperature and nutritional effects on the setting rate of larvae of the tropical oyster, Crassostrea iredalei (Faustino). Aquaculture, 184: 105-114.
Doroudi, M.S. and Southgate, P.C. (2002) The effect of chemical cues on settlement behaviour of blacklip pearl oyster (Pinctada margaritifera) larvae. Aquaculture, 209: 117–124.
Faimali, M., Garaventa, F., Terlizzi, A., Chiantore, M. and Cattaneo-Vietti, R. (2004) The interplay of substrate nature and biofilm formation in regulating Balanus amphitrite Darwin, 1854 larval settlement. Journal of Experimental Marine Biology and Ecology, 306: 37–50.
Ganesan. A.M., Alfaro A.C., Brooks J.D. and Higgins C.M. (2010) The role of bacterial biofilms and exudates on the settlement of mussel (Perna canaliculus) larvae. Aquaculture, 306: 388-392.
García-Lavandeira, M., Silva, A., Abad, M., Pazos, A.J., Sánchez, J.L. and Pérez- Parallé, M.L. (2005) Effects of GABA and epinephrine on the settlement and metamorphosis of the larvae of four species of bivalve molluscs. Journal of Experimental Marine Biology and Ecology, 316: 149–156.
Hadfield, M.G., 1978. Metamorphosis in marine molluscan larvae: an analysis of stimulus and response. In: Chia, F.S. and Rice, M.E. Eds, Settlement and Metamorphosis of Marine Invertebrate Larvae. 165–175. Elsevier, New York.
Hadfield, M.G. (1984) Settlement requirement of molluscan larvae: new data on chemical and genetic roles. Aquaculture, 39: 283–298.
Hadfield, M.G. and Paul, V.J. (2001). Natural chemical cues for settlement and metamorphosis of marine-invertebrate larvae. In: McClintock, J.B., Baker, B.J. (Eds.), Marine Chemical Ecology. 431–461. CRC press, New York.
Henderson, B.A. (1983) Handling and Remote Setting Techniques for the Pacific oyster larvae, Crassostrea gigas. Master’s thesis, Department of Fisheries and Wildlife, 37. Oregon State University. OR
Hidu, H. and Haskin, H. (1971) Setting of the American oyster related to environmental factors and larval behaviour. Proceeding of National Shellfish Association, 61: 35–49.
Hur, Y.B., WI, C.H., KIM, T.I., Chun, C.Y., Hwang, M.S., Kim, Y.O. and Choe, S.W. (2002). Effect of hardening on growth survival of spats of the Pacific oyster, Crassostrea gigas artificially produced in Autumn season. bulletin of National Fisheries Research and Development Agency, 61: 77-84.
Hur, Y.B., Min, K.S., Kim, T.E., Lee, S.J. and Hur, S.B. (2008) Larvae growth and biochemical composition change of the Pacific oyster Crassostrea gigas, larvae during artificial seed production. Journal of Aquaculture, 21: 203-212.
Jensen, R.A., Morse, D.E., Petty, R.L. and Hooker, N. (1990) Artificial induction of larval metamorphosis by free fatty acids. Marine Ecological Progress of Service, 67: 55–71.
Johnson, C.R., Muir, D.G. and Reysenbach, A.L. (1991) Characteristic bacteria associated with surfaces of coralline algae: a hypothesis for bacterial induction of marine invertebrate larvae. Marine Ecological Progress of Service, 74: 281–294.
Jones, G.G. and Jones, B.L. (1988) Advances in Remote Setting of Oyster Larvae. 88. Aquaculture Association of BC, Nanaimo, BC.
Keough, M.J. and Raimondi, P.T. (1996) Responses of settling invertebrate larvae to bioorganic films: Effects of large-scale variation in films. Journal of Experimental Marine Biology and Ecology, 207: 59–78.
Laing, I. (1995) Effect of food supply on oyster spat fall. Aquaculture, 131: 315–324.
Leitz, T. and Wagner, T. (1993) The marine bacterium Alteromonas espejiana induces metamorphosis of the hydroid Hydractinia echinate. Marine Biology, 115: 173–178.
Lund, D.S. (1971) Laboratory studies on setting of the Pacific Oyster Crassostrea gigas. Master’s thesis, Department of Fisheries and Wildlife, 85. Oregon State University, OR.
Maki, J.S., Rittschof, D., Schmidt, A.R., Snyder, A.G. and Mitchell, R. (1989) Factors controlling attachment of bryozoan larvae: a comparison of bacterial films and unfilmed surfaces. Biological Bulletin, 177: 295–302.
Martinez, G., Aguilera, C. and Campos, E.O. (1999) Induction of settlement and metamorphosis of the scallop Argopecten purpuratus Lamarck by excess K+ and epinephrine: energetic costs. Journal of Shellfish Research, 18: 41–46.
Min, K.S., Chang, Y.J., Park, D.W., Jung, C.G., Kim, D.H. and Kim, G.H. (1995) Studies on rearing conditions for mass seedling production in Pacific oyster larvae, Crassostrea gigas (Thunberg). bulletin of National Fisheries Research and Development Agency, 49: 91-111.
Min. S.K., Kim, T.I., Hur, S.B., Hur, Y.B., Chun, C.Y. and Kim, D.H. (1999) Growth and survival of the artificial and natural seeding in the Pacific oyster, Crassostrea gigas (Thunberg). bulletin of National Fisheries Research and Development Agency, 57: 43-53.
Morse, D.E. (1990) Recent progress in larval settlement and metamorphosis: closing the gaps between molecular biology and ecology. Bulletin of marine Science, 46: 465–483.
Paul, J.D. (1980) Salinity–temperature relationship in queen scallop Chlamys opercularis. Marine Biology, 56: 295–300.
Pawlik, J.R. (1992) Chemical ecology of the settlement of benthic marine invertebrate. Oceanogr. Marine Annual Review, 30: 273–335.
Qian, P.Y. (1999) Larval settlement of polychaetes. Hydrobiologia, 402: 239–253.
Quayle, D.B. and Newkirk, G.F. (1989) Farming Bivalve Molluscs: Methods for Study and Development. Advances in World Aquaculture, Vol. 1. The World Aquaculture Society In Assoc. with The International Development Research Centre. 294.
The World Aquaculture Society. Baton Rouge, LA. Rodriguez, S.R., Ojeda, F.P. and Inestrosa, N.C. (1993) Settlement of benthic marine invertebrates. Marine Ecological Progress of Service, 97: 193– 207.
Supan, J. (1987). Using Remote Setting to produce Seed Oyster in Louisiana and the Gulf Coastal Region. Louisiana Sea Grant College Program. 47. Louisiana State University, Baton Rouge, LA.
Swanson, R.L., de Nys, R., Huggett, M.J., Green, J.K., Steinberg, P.D. (2006) In situ quantification of a natural settlement cue and recruitment of the Australian sea urchin Holopneustes purpurascens. Marine Ecological Progress of Services, 314: 1–14.
Tamburri, M.N., Zimmer-Faust, R.K. and Tamplin, M.L. (1992) Natural sources and properties of chemical inducers mediating settlement of oyster larvae: a re-examination. Biological Bulletin, 183: 327–338.
Tan, S.H. (1993) Aspects on Biology and Larval Development of Tropical Oyster, Crassostrea belcheri (Sowerby). Master’s thesis, School of Biological Sciences University. 221. Sc. Malaysia.
Tettelbach, S.T. and Rhodes, E.W. (1981) Combined effects of temperature and salinity on embryos and larvae of the northern bay scallop, Argopecten irradians irradians. Marine Biology, 63: 249–256.
Todd, C.D. (1985) Settlement-timing hypothesis: reply to Grant and Williamson. Marine Ecological Progress of Services, 23: 197–202.
Walch, M., Weiner, R.M., Colwell, R.R. and Coon, S.L. (1999) Use of l-DOPA and soluble bacterial products to improve set of Crassostrea virginica (Gmelin, 1791) and C. gigas (Thunberg, 1793). Journal of Shellfish Research, 18: 133–138.
Weiner, R.M., Walch, M., Labare, M.P., Bonar, D.B. and Colwell, R.R. (1989) Effect of biofilms of the marine bacterium Alteromonas colwelliana (LST) on set of the oysters Crassostrea gigas (Thunberg, 1793) and C. virginica (Gmelin, 1791). Journal of Shellfish Research, 8: 117–123.
Yu, X., He, W., Gu, J.D., He, M. and Yan, Y. (2008) The effect of chemical cues on settlement of pearl oyster Pinctada fucata martensii (Dunker) larvae. Aquaculture, 277: 83-91.
Zhao, B. and Qian, P.Y. (2002) Larval settlement and metamorphosis in the slipper limpet Crepidula onyx (Sowerby) in response to conspecific cues and the cues from biofilm. Journal of Experimental Marine Biology and Ecology, 269: 39–51.
Zimmer-Faust, R.K. and Tamburri, M.N. (1994) Chemical identity and ecological implications of a waterborne, larval settlement cue. Limnology and Oceanography, 39: 1075–1087.
유성규. 2000. 천해양식. p 118-119, 구덕출판사, 부산
