Mussels have been commonly used in bioassay for quality assessments of environment. Moreover, several standard protocols for the developmental bioassay of bivalves have been proposed. In this study, the EC50 of polycyclic aromatic hydrocarbons (PAHs) was determined using mussel, Mytilus galloprovincialis embryonic developmental bioassay. To determine the sensitivity of M. galloprovincialis, their fertilized eggs were exposed to five PAHs (2-metylnaphthalene, fluorene, dibenzothiophene, phenanthrene, pyrene). The EC50 of 2-metylnaphthalene, fluorene, dibenzothiophene, phenanthrene, and pyrene were 232, 273, 67.9, 43.2, and 33.1 μg/L, respectively. The overall sensitivity of M. galloprovincialis in the present developmental bioassay was similar to or more sensitive than that of other marine organisms commonly used in aquatic bioassays. The results of this study could be provide with fundamental data of setting standard for protection of marine life and or can use prediction the aquatic toxicity of PAHs.
Abernethy, S., A. Bobra, W. Shiu, P. Wells, D. Mackay (1986) Acute lethal toxicity of hydrocarbons and chlorinated hydrocarbons to two planktonic crustsceans: The key roll of organism-waterpartitioning. Aquatic Toxicology, 8: 163-174.
Anderson, J.W., J.M. Neff, B.A. Cox, H.E. Tatem, G.M. Hightower (1974) Characteristics of dispersions and water-soluble extracts of crude and refined oils and their toxicity to estuarine crustaceans and fish. Marine Biology, 27: 75-88.
ASTM (American Society for Testing and Materials) (1994) Standard guide for conducting acute toxicity tests starting with embryos of four species of saltwater bivalve molluscs. ASTM E724-94. American Society for Testing and Materials, Philadelphia.
Barata, C., J. Navarro, I. Varo, M. Riva, S. Arun, C. Porte (2005) Changes in antioxidant enzyme activities, fatty acid composition and lipid peroxidation in Daphnia magna during the aging process. Comparative Biochemistry and Physiology, B140: 81–90.
Battelle (2007) Sediment Toxicity of Petroleum Hydrocarbon Fractions. Massachusetts Department of Environmental Protection, Office of Research and Standards.
Beiras, R., Bellas, J., Fernάndez, N., Lorenzo, J.I., Cobelo-Garcia, A. (2003a) Assessment of costal marine pollution in Galicia (NW Iberian Peninsula); metal concentration in seawater, sediment and mussels (Mytilus galloprovincialis) versus embryo-larval bioassays using Paracentrotus lividus and Ciona intestinalis. Marine Environmental Research, 56: 531-553.
Beiras, R., Fernάndez, N., Bellas, J., Besada, V., Gonzάlez-Quijano, A. and Nunes, T. (2003b) Integrative assessment of marine pollution in Galician estuaries using sediment chemistry, mussel bioaccumulation, and embryo-larval toxicity bioassays. Chemosphere, 52: 1209-1224.
Beiras, R. and Albentosa, M. (2004) Inhibition of embryo development of the commercial bivalves Ruditapes decussatus and Mytilus galloprovincialis by trace metals; Implication for the implementation of seawater quality criteria. Aquaculture, 230: 205-213.
Beiras, R. and J. Bellas (2008) Inhibition of embryo development of the Mytilus galloprovincialis marine mussel by organic pollutants; assessment of risk for its extensive culture in the Galician Rias. Aquaculture, 277(3-4): 208-212.
Bellas J., L. Saco-Álvarez, Ó., Nieto, R. Beiras (2008) Ecotoxicological evaluation of polycyclic aromatic hydrocarbons using marine invertebrate embryo– larval bioassays. Marine Pollution Bulletin, 57: 493-502.
Boehm, P.D., J.M. Neff, D.S. Page (2007) Assessment of polycyclic aromatic hydrocarbon exposure in the waters of Prince William Sound after the Exxon Valdez oil spill: 1989-2005. Marine Pollution Bulletin, (54): 339-367.
Boese B, J. Lamberson, R. Swartz, R. Ozretich (1997). Photoinduced toxicity of fluoranthene to seven marine benthic crustaceans. Archives of Environmental Contamination and Toxicology, 32: 389–393.
Brooke, L.T. (1994) Acute Phenanthrene Toxicity to Daphnia magna U.S.EPA Contract No.68-C1-0034, Work Assignment No.2-14, to R.L.Spehar, U.S.EPA, Duluth, MN: 11 p.
Cachada, A., R. Pereira, E. Ferreira da Silva, A.C. Duarte (2014) The prediction of PAHs bioavailability in soils using chemical methods: State of the art and future challenges. Science of The Total Environment, 472: 463-480.
Castro-Jimenez, J., N. Berrojalbiz, J. Wollgast, J. Dachs (2012) Polycyclic aromatic hydrocarbons (PAHs) in the Mediterranean Sea: Atmospheric occurrence, deposition and decoupling with settling fluxes in the water column. Environmental Pollution, 166: 40-47.
Call, D.J., L.T. Brooke, S.L. Harting, S.H. Poirier, D.J. McCauley (1986) Toxicity of Phenanthrene to Several Freshwater Species. Rep.to Battelle Memorial Res.Inst., Columbus, OH, in Partial Fulfillment of Work Assignment No.45, Task Order No.4 of Subcontract No.F4114(8834)-411: 15 p.
Di Toro, D.M., J.A. McGrath, D.J. Hansen (2000) Technical basis for narcotic chemicals and polycyclic aromatic hydrocarbon criteria I. Water and tissue. Environmental Toxicology and Chemistry, 19: 1951– 1970.
Donkin, P., J. Widdows, S.V. Evans, C.M. Worrall, M. Carr (1989) Quantitative Structure-Activity Relationships for the Effect of Hydrophobic Organic Chemicals on Rate of Feeding by Mussels (Mytilus edulis). Aquatic Toxicology, 14(3): 277-294.
Eastman D, G. Booth, M. Lee (1984) Toxicity, accumulation, and elimination of polycyclic aromatic sulfur heterocycles in Daphnia magna. Archives of Environmental Contamination and Toxicology, 13: 105–111.
Emery, V.L., Jr., T.M. Dillon (1996) Chronic toxicity of phenanthrene to the marine polychaete worm, Nereis (Neanthes) arenaceodentata. Bulletin of Environmental Contamination and Toxicology, 56: 265-270.
Faria, M., M.A. Lopez, M. Fernandez-Sanjuan, S. Lacorte, C. Barata (2010) Comparative toxicity of single and combined mixtures of selected pollutants among larval stages of the native freshwater mussels (Unio elongatulus) and the invasive zebra mussel (Dreissena polymorpha) Science of The Total Environment, 408(12): 2452-2458.
Finger, Susan E., E.F. Little, M.G. Henry, J.F. Fairchild, T.P. Boyle. (1985) Comparison of laboratory and field assessment of fluorene - Part I: Effects of fluorene on the survival, growth, reproduction, and behavior of aquatic organisms in laboratory tests. Pages 120-133 In: T.P. Boyle, Ed., Validation and Predictability of Laboratory Methods for Assessing the Fate and Effects of Contaminants in Aquatic Ecosystems. ASTM STP 865. American Society for Testing and Materials, Philadelphia, PA.
Geffard, O., His, E., Budzinski, H., Seaman, M. and Garrigues, P . (2001) Qualité biologique de l'eau de merévalue in situ par le test embryo-larvaire de Crassostrea gigas et Mytilus galloprovincialis: Comptes Rendus de l'Académie des Sciences, Serie III. Sciences de la Vie, 324: 1149-1155.
Geiger, J.G. A.L. Buikema, Jr. (1982) Hydrocarbons depress growth and reproduction of Daphnia pulex (Cladocera). Canadian Journal of Fisheries and Aquatic Sciences, 39: 830-836.
Goldberg, E.D. and Bertine, K.K. (2000) Beyond the mussel watch - new directions for monitoring marine pollution. The Science of the Total Environment, 247(2-3): 165-174.
Guitart, C., P. Frickers, J. Horrillo-Caraballo, R.J. Law, J.W. Readman (2008) Characterization of sea surface chemical contamination after shipping accidents. Environmental Science & Technology, 42(7): 2275-2282.
His, E., Seaman, M.N.L. and Beiras, R. (1996) A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment. Water Research, 31(2): 351-355.
Hur, Y.B. and Hur, S.B. (2000) Development and growth of larvae of four bivalve species. Journal of Aquaculture, 13(2): 119-128. [in Korean]
International Tanker Owners Pollution Federation Ltd. (ITOPF) (2008) The environmental impact of the Hebei Sprit oil spill, Taean, South Korea. Available from: <http://www.itopf.com/news-and-events/documents/ HEBEISPIRIT – EnvironmentalImpact.pdf>. Jha, A.N., Cheung, V.V., Foulkes, M.E., Hill, S.J. and
Jha, A.N., Cheung, V.V., Foulkes, M.E., Hill, S.J. and Depledge, M.H. (2000) Detection of genotoxins in the marine environment: adoption and evaluation of an integrated approach using the embryo–larval stages of the marine mussel, Mytilus edulis. Mutation Research, 464(2): 213-228.
Jung, K.H., J.K. Kim, J.H. Noh, J.W. Eun, H.J. Bae, M.G. Kim, Y.G. Chang, Q. Shen, S.J. Kim, S.H. Kwon, W.S. Park, J.Y. Lee, S.W. Nam (2013) Characteristic molecular signature for the early detection and prediction of polycyclic aromatic hydrocarbons in rat liver. Toxicology Letters, 216(1): 1-8.
Kanaki, M., A. Nikolaou, C.A. Makri, D.F. Lekkas (2007) The occurrence of priority PAHs, nonylphenol and octylphenol in inland and coastal waters of Central Greece and the Island of Lesvos. Desalination, 210(1-3): 16-23.
Kennedy, C.J. (1990) Toxicokinetic study of chlorinated phenols and polycyclic aromatic hydrocarbons in rainbow trout (Oncorhynchus mykiss). Simon Fraser University.
Kennish, M.J. (1992) Ecology of Estuaries: Anthropogenic Effects. CRC Press, Boca Raton, FL.
Kurt, P .B. and Ozkoc, B. (2004) A survey to determine levels of chlorinated pesticides and PCBs in mussels and seawater from the Mid-Black Sea Coast of Turkey. Marine Pollution Bulletin, 48(11-12): 1076-1083.
Kwon, J.H., T. Wuethrich, P. Mayer, B.I. Escher (2009) Development of a dynamic delivery method for in vitro bioassays. Chemosphere, 76: 83–90.
Latimer, J.S., J. Zheng, (2003) The sources, transport, and fate of PAHs in the marine environment. In: Douben, P.E. (Ed.), PAHs: An Ecotoxicological Perspective. John Wiley, London, pp. 9–33.
Lim, L., O. Wurl, S. Karuppiah, J.P. Obbard (2007) Atmospheric wet deposition of PAHs to the sea-surface microlayer. Marine Pollution Bulletin, 54(8): 1212-1219.
Meador, J.P. (2003) Bioaccumulation of PAHs in marine invertebrates. In: Douben, P.E. (Ed.), PAHs: An Ecotoxicological Perspective. John Wiley, London, pp. 147–171.
Millemann, R.E., W.J. Birge, J.A. Black, R.M. Cushman, K.L. Daniels, P.J. Franco, J.M. Giddings, J.F. McCarthy, and A.J. Stewart (1984) Comparative acute toxicity to aquatic organisms of components of coal-derived synthetic fuels. Transactions of the American Fisheries Society, 113: 74-85.
Moreau, C.J., P.L. Klerks, C.N. Haas (1999) Interaction between phenanthrene and zinc in their toxicity to the sheepshead minnow (Cyprinodon variegatus). Archives of Environmental Contamination and Toxicology, 37: 251-257.
Nicholson, S. and Szefer, P. (2003) Accumulation of metal in the soft tissues, byssus and shall of the mytilid mussel Perna viridis (Bivalvia: Mytilidae) from polluted and uncontaminated locations in Hong Kong coastal waters. Marine Pollution Bulletin, 46: 1035-1048.
Pelletier, M.C., R.M. Burgess, K.T. Ho, A. Kuhn, R.A. McKinney, S.A. Ryba (1997) Phototoxicity of Individual Polycyclic Aromatic Hydrocarbons and Petroleum to Marine Invertebrate Larvae and Juveniles. Environmental Toxicology and Chemistry, 16(10): 2190-2199.
Qiu,Y.W., G. Zhang, G.Q. Liu, L.L. Guo, X.D. Li, O. Wai (2009) Polycyclic aromatic hydrocarbons (PAHs) in the water column and sediment core of Deep Bay, South China. Estuarine, Coastal and Shelf Science, 83(1): 60-66.
Rossi, S.S., J.M. Neff. August (1978) Toxicity of polynuclear aromatic hydrocarbons to the polychaete Neanthes arenaceodentata. Marine Pollution Bulletin, 9(8): 220-223
Smith, S.B., J.F. Savino, M.A. Biouin (1988) Acute toxicity to Daphnia pulex of six classes of chemical compounds potentially hazardous to Great Lakes biota. Journal of Great Lakes Research, 14: 394-404.
Smolders, R., Bervoets, L. and Blust, R. (2004) In situ and laboratory bioassays to evaluate the impact of effluent discharges on receiving aquatic ecosystems. Environmental Pollution, 132(2): 231-243.
Song, M.K., M. Song, H.S. Choi, Y.J. Kim, Y.K. Park, J.C. Ryu (2012) Identification of molecular signatures predicting the carcinogenicity of polycyclic aromatic hydrocarbons (PAHs). Toxicology Letters, 212(1): 18-28.
Sung, C.G., G.B. Kim, J.Y. Seo, C.H. Lee, T.K. Ryu, G.M. Han, J.W. Choi, Y.H. Kim (2005) Optimal conditions of the embryonic development of mussel, Mytilus galloprovincialis. Korean Journal of Malacology, 21(1): 25-31. [in Korean]
Sung, C.G., G.B. Kim, C.H. Lee (2006) Effect of heavy metals on embryonic development in the mussel, Mytilus galloprovincialis. Korean Journal of Malacology, 22(2): 167-173.
Sunita R. Nadella, M. Tellis, R. Diamond, S. Smith, A. Bianchini, C.M. Wood (2013) Toxicity of lead and zinc to developing mussel and sea urchin embryos: Critical tissue residues and effects of dissolved organic matter and salinity Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 158(2): 72-83.
Trucco, R.G., F.R. Engelhardt, and B. Stacey (1983) Toxicity, accumulation and clearance of aromatic hydrocarbons in Daphnia pulex. Environmental Pollution (Series A), 31: 191-202.
UNEP, 2003. 잔류성유기오염물질의 국제적 규제를 위한 스톡홀름 협약, UNEP PRESS.
USEPA (1995) Short-Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to West Coast Marine and Estuarine Organisms. EPA/600/R-95-136.
Veith, G.D., D.J. Call, L. Brooke (1983) Structure-toxicity relationships for the fathead minnow, Pimephales promelas: narcotic industrial chemicals. Canadian Journal of Fisheries and Aquatic Sciences, 40: 743–748.
Walker, C.H., S.P. Hopkin, R.M. Sibly, D.B. Peakall (2001) Principles of Ecotoxicology, second ed. Taylor & Francis, London.
Wang, Z., B.P. Hollebone, M. Fingas, B. Fieldhouse, L. Sigouin, M. Landriault, P. Smith, J. Noonan, G. Thouin (2003) Characteristics of spilled oils, fuels, and petroleum products: Composition and properties of selected oils. EPA/600/R-03/072. U.S. Environmental Protection Agency, Washington, DC.
Wedderburn, J., McFadzen, I., Sanger, R.C., Beesley, A., Heath, C., Hornsby, M. and Lowe, D. (2000) The field application of cellular and physiological biomarkers, in the mussel Mytilus edulis, in conjunction with early life stage bioassays and adult histopathology. Marine Pollution Bulletin, 40(3): 257-267.
Weinstein, J.E., K.D. Polk (2001) Phototoxicity of Anthracene and Pyrene to Glochidia of the Freshwater Mussel Utterbackia imbecillis. Environmental Toxicology and Chemistry, 20(9): 2021-2028.
