ISSN : 1225-3480
코끼리조개의 대량 인공종묘생산을 추진하기 위한 기초연구로서 수정란, 유생 및 치패의 발달을 조사하였다. 생식체는잘 성숙한 어미에서 추출하여 희석 정자 용액으로 수정하였으며, 발생유생은 수온 19 ± 1℃로 유지하였다. 수정란의 난경은 평균 81.6 μm 이었으며, 수정 후 14시간 만에 담륜자유생이 되어 부상하고, 27시간 만에 D상 유생 (각장 116 μm) 으로 발달하고, 20일 후에는 각장 311.2 μm로 성장하여 바닥에 안착하였다. 18.5-21.5℃에서 정상적으로 부화 및 유생사육이 가능하였으며, 채묘기질은 모래가 우수하였으나 캐시미론도 양호하였다. 부유유생이 착저한 후 108일간 사육한결과 각장 13 mm, 전중량 0.4 g으로 성장하였고 일간성장률은 각장 117.5 μm 이었으며, 자연해수로 연중 실내육성이 가능하였다.
The development of Japanese geoduck, Panopea japonica, grown under culture conditions, has been examined through the morphological characteristics in fertilized egg, larvae and juvenile. Gametes were stripped from ripe broodstock and placed into two separate containers. Eggs were washed through a 40 μm sieve and fertilized with dilute sperm solution. Developing larvae were maintained at 19 ± 1℃. Fertilized eggs with 81.6 μm diameter developed to trochophores within 14 h and to D-stage larvae (116 μm shell length) within 27 h. Larvae were spontaneously settled at shell length of 311 μm after 20 days. The hatching from fertilized eggs and larval rearing were normally available in 18.5-21.5℃, and the growth was good in a cashmilon substrate, as well as sand. After rearing of day 108 from metamorphosis, the shell length of juvenile P. japonica reached 13 mm, and growth rate of shell length of the juvenile was 117.5 μm/d.
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