ISSN : 1225-3480
The climate change causes the mass mortality of farmed fish and shellfish. In order to find genes involved in physiological response to temperature stress, we have performed sequencing of transcriptome in the pacific abalone (Haliotis discus hannai) after thermal stimulation. In the present study, we present a focused analysis on the heat shock factor 1(HSF1) which is rapidly induced to activate heat shock response (HSR) in host cells under thermal stress as a major transcriptional regulator of heat shock proteins (HSPs). The H. discus hannai HSF1 (HdhHSF1) cDNA contains an open reading frame of 1473 bp, encoding a protein of 490 aa. The deduced amino acid sequence of HdhHSF1 shared the high identity with sequences of the H. asinina (ABR15461.1, 81.6% identity) and H. diversicolor (AGN53400.1, 81.2% identity). The phylogenetic tree of HSF1 revealed that these proteins were divided into two clusters, one comprising vertebrate and mollusc proteins, and the other one containing insecta proteins. The qRT-PCR detected HdhHSF1 mRNA expressions in hemocyte and gill tissues examined. The mRNA expression of HdhHSF1 showed a significant increase at 20°C in gill and 30°C in hemocyte. The expression of HdhHSF1 increased significantly after thermal stress of 6 h in gill, and also increased significantly in hemocyte at 30°C. The present study is the first to report on the molecular cloning, characterization and tissue expression analysis of HSF1 in H. discus hannai. The results might provide new insights into the regulation of HSR in mollusca.
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