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IFN-γ mRNA Expression in Tuberculous Pleural Lymphocytes After in vitro Stimulation with M.tuberculosis Antigens

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2004, v.57 no.1, pp.25-31




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Abstract

Background:IFN-γ is the main effector mediator of the host immune response against Mycobacterium tuberculosis. Evaluating the IFN-γ gene expression in response to M. tuberculosis antigens may help in elucidating the host defense mechanism against M. tuberculosis and in the development of a vaccine. Methods:The IFN-γ mRNA expression in the lymphocytes obtained from pleural effusions from tuberculous pleurisy patients (TB-PLC) after in vitro stimulation with whole cell M. tuberculosis(H37Rv), purified protein derivatives(PPD), man-lipoarabinamman (man-LAM), ara-LAM and Antigen 85B(Ag85B) were evaluated. The degree of IFN-γ mRNA expression was determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results:M. tuberculosis induced the expression of IFN-γ mRNA in the TB-PLC in time and dose dependent manners. The PPD and Ag85B induced high levels of IFN-γ mRNA expression in the TB-PLC. However, man-LAM inhibited IFN-γ mRNA expression in the TB-PLC, while ara-LAM did not. Conclusion:IFN-γ mRNA expression in TB-PLC is stimulated by PPD and Ag85B, but inhibited by man-LAM.(Tuberc Respir Dis 2004; 57:25-31)

keywords
Interferon-gamma, Tuberculosis, PPD, Ag85B, LAM.

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Tuberculosis & Respiratory Diseases