바로가기메뉴

본문 바로가기 주메뉴 바로가기

Tuberculosis & Respiratory Diseases

Article Contents

Prev Next
e-Submission

Vol.57 No.5
Citation Share

The Preventive Effect of Allergic Inflammation by Induction of Oral Tolerance in a Mouse Model of Chronic Asthma

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2004, v.57 no.5, pp.425-433










& (2004). The Preventive Effect of Allergic Inflammation by Induction of Oral Tolerance in a Mouse Model of Chronic Asthma. Tuberculosis & Respiratory Diseases, 57(5), 425-433.
  • Downloaded
  • Viewed
PDF Download

Abstract

Background : Induction of oral tolerance (OT) has been known to prevent allergic inflammation in acute asthma model within 4 weeks. However it is remained whether induction of OT may effectively prevent allergic inflammation in chronic asthma model over 4 weeks. We observed the effect of induction of OT on allergic inflammation and airway remodeling in chronic asthma model up to 8 weeks. Methods : 5-week-old female BALB/c mice divided into 4 groups-control group, asthma group, low dose OT group, and high dose OT group. To induce oral tolerance mice were fed ovalbumin (OVA) before sensitization with OVA and aluminum hydroxide-1 mg for 6 consecutive days in the low dose OT group and 25 mg once in the high dose OT group. Mice in the asthma group were fed phosphate buffered saline instead of OVA. After sensitization followed by repeated challenge with aerosolized 1% OVA during 6 weeks, enhanced pause (Penh), inflammatory cells, IL-13, and IFN-γ levels in bronchoalveolar lavage (BAL) fluids as well as OVA-specific IgE, IgG1, and IgG2a levels in serum were measured. In addition the degree of goblet cell hyperplasia and peribronchial fibrosis were observed from lung tissues by PAS and Masson's trichrome stain. Results : Both OT groups showed a significant decrease in Penh, inflammatory cells, IL-13, and IFN-γ levels in BAL fluids as well as OVA-specific IgE, IgG1, and IgG2a levels in serum compared with the asthma group (P<0.05). In addition, the degree of goblet cell hyperplasia and peribronchial fibrosis were significantly attenuated in both OT groups compared with the asthma group (P<0.01). Conclusion : These results suggest that induction of OT may effectively prevent allergic inflammation as well as airway remodeling even in chronic asthma model up to 8 weeks.(Tuberc Respir Dis 2004; 57:425-433)

keywords
Allergic asthma, Oral tolerance, Ovalbumin, Airway remodeling.마우스 만성천식모델에서 경구면역관용 유도에 의한 알레르기 염증의 예방효과가톨릭대학교 의과대학 내과학교실김진숙, 이정미, 김승준, 이숙영, 권순석, 김영균, 김관형, 문화식, 송정섭, 박성학The Preventive Effect of Allergic Inflammation by Induction of Oral Tolerance in a

Reference

1.

(2001) Murine model of chronic human asthma,

2.

(2000) Asthma.From bronchoconstriction to airways inflammation and remodeling,

3.

(2000) Oral tolerance,

4.

(2001) Sensitization and tolerance,

5.

(1999) Oral tolerance in disease,

6.

(2002) Preventive and therapeutic effects of oral tolerance in a murine model of asthma,

7.

(1999) gammadelta T cells regulate mucosally induced tolerance in a dose-dependent fashion,

8.

(1999) Orally induced peripheral nonresponsiveness is maintained in the absence of functional Th1 or Th2 cells,

9.

(2001) The effect of allergen-induced airway inflammation on airway remodeling in a murine model of allergic asthma,

10.

(2003) Morphometric analysis of mouse airways after chronic allergen challenge,

11.

(2002) Dysfunction and remodeling of the mouse airway persist after resolution of acute allergen induced airway inflammation,

12.

(2001) Remodeling of the airway epithelium in asthma,

13.

(2000) Immunopathology of intestinal helminth infection,

14.

(1997) The anatomical basis of intestinal immunity,

15.

(2001) Food for thought can immunological tolerance be induced to treat asthma,

16.

(1998) Prevention of lung eosinophilic inflammation by oral tolerance,

17.

(2001) Suppression of asthma- like responses in different mouse strains by oral tolerance,

18.

(1995) Molecular mechanisms underlying functional T-cell unresponsiveness,

19.

(1998) Positive versus negative signaling by lymphocyte antigen receptors,

20.

(jimmunol1986) Two types of murine helper T cell clone Definition according to profiles of lymphokine activities and secreted proteins,

21.

(1998) High-dose oral tolerance prevents antigen-induced eosinophil recruitment into the mouse airways,

Tuberculosis & Respiratory Diseases

e-Submission OA Policy