Aquaporin in bleomycin induced lung injury

Background : Aquaporins (AQPs) may play a role in the pathogenesis of pulmonary inflammation and edema. This study investigated the role ofAQPs in acute lung injury following bleomycin inhalation in rats.Methods : Sprague-Dawley rats were treated via inhalation with 10 U/kg bleomycin hydrochloride dissolved in 5 ml of normal saline. The control rats were treated with 5 ml normal saline. The animals (n = 6-8 rats per group) were sacrificed at 4, 7, and 14 d. The changes in AQP1, AQP4, and AQP5 expression levels over time were analyzed by Western blotting. The nitrate and nitrite concentrations in the bronchoalveolar lavage fluid (BALF) were measured using a modified Griess reaction. ELISA was used to check cytokines.Results : The respiration rates were significantly higher 4 and 7 days after the bleomycin treatment compared with those of the control rats. The tidal volume was lower in rats at 4 days after the bleomycin treatment, and the wet/dry weights of the lung were significantly higher than those of the control group. The nitrite and nitrate concentrations in the BALF from the rats at 4 days after exposure to bleomycin were greater than those from the saline-treated rats. Immunoblotting studies demonstrated that the AQP1 and AQP4 expression levels were lower in the rats at 4 days. However, the AQP4 expression level was higher at 7 days. The AQP5 expression level increased at 4, 7 and 14 days after the bleomycin treatment.Conclusion : This study demonstrates that AQPs are expressed differently in bleomycin-induced pulmonary edema. (Tuberc Respir Dis 2006; 60: 330-336)

keywords: Aquaporin, Nitric oxides, acute lung injury, Aquaporin, Nitric oxides, acute lung injury

Reference

Doyle RL, (1995) Identification of patients with acute lung injury:predictors of mortality,

Jules-Elysee K, (1990) Bleomycin-induced pulm onary toxicity, Clin Chest Med

Piguet PF, (1993) Interleukin 1 receptor antagonist(IL-1ra)prevents or cures pulmonary fibrosis elicited in mice by bleomycin or silica,

Smith RE, (1995) A role for C-C chemokines in fibrotic lung disease,

Piguet PF, (1989) Tumor necrosis factor/cachectin plays a key role in bleomycin-induced pneumopathy and fibrosis,

King LS, (1996) Aquaporin-1 water channel protein in lung:ontogeny,steroid-induced expression,and distribution in rat,

Nielsen S, (1997) Aquaporins in complex tissues cellular and subcellular distribution in respiratory tract and glands of rat,

Towne JE, (2000) Decreased expression of aquaporin(AQP)1 and AQP5 in mouse lung after acute viral infection,

Smith PK, (1985) Measurement of protein using bicinchninic acid,

10.

Dickason RR, (1994) Enhanced detection of human interluekin-5 in biological fluids utilizing murine monoclonal antibodies which delineate didtinct neutralizing epitope,

11.

Matthay MA, (2005) Acute lung injury and the acute respiratory distress syndrome:four decades of inquiry into pathogenesis and rational management,

12.

Ashbaugh DG, (1967) Acute respiratory distress in adults,

13.

The Acute Respiratory Distress Syndrome Network, (2000) Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome,

14.

Brower RG, (2004) Higher versus lower positive end-expiratory pressures in patients with the acute respiratory distress syndrome,

15.

Zimmerman GA, (2003) Pathogenesis of sepsis and septic-induced lung inury, Marcel Dekker

16.

Jang AS, (2004) Expression of nitric oxide synthase,aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation,

17.

Moncada S, (1989) Biosynthesis of nitric oxide from L-arginine:a pathway for the regulation of cell function and communication,

18.

Moncada S, (1991) Nitric oxide:physiology,pathophysiology and pharmacology,

19.

Haddad IY, (1994) Quantification of nitrotyrosine levels in lung secretions of patients and animals with acute lung injury,

20.

Agre P, Purification and partial characterization of the Mr 30 000 integral membrane protein associated with the erythrocyte Rh,

21.

Denker BM, and partial characterization of a novel Mr 28 000 integral membrane protein from erythrocytes and renal tubules,

22.

Preston GM, (1991) Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons:member of an ancient channel family,

23.

Preston GM, (1992) Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein,

24.

Jung JS, (1994) Molecular structure of the water channel through aquaporin CHIP,

25.

van Hoek AN, (1998) Freez-fracture analysis of plasma membrane of CHO cells stably expressing aquaporin1-5,

26.

Yang B, (1996) The mercurial insensitive water channel(AQP4)forms orthogonal arrays in stably transfected Chinese hamster ovary cells,

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Article Contents

Vol.60 No.3

Aquaporin in bleomycin induced lung injury

Abstract

Reference

Tuberculosis & Respiratory Diseases