바로가기메뉴

본문 바로가기 주메뉴 바로가기

Tuberculosis & Respiratory Diseases

Article Contents

Prev Next
e-Submission

Vol.61 No.1
Citation Share

Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2006, v.61 no.1, pp.54-59





& (2006). Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues. Tuberculosis & Respiratory Diseases, 61(1), 54-59.
  • Downloaded
  • Viewed
PDF Download

Abstract

Background: Although there have been several studies regarding the clinical value of an automated TB-PCR study using sputum, bronchial washing, and other body fluid samples for the detection of pulmonary tuberculosis, there are only a few reports on the use of fresh tissue samples.Materials and methods: The acid-fast bacilli stain(AFB), tuberculosis culture, automated TB-PCR study, and histopathology examination were performed in 42 fresh tissue samples.Results: Among the 42 cases, 18 cases were diagnosed with tuberculosis based on the clinical findings. Sixteen of the 18 cases were TB-PCR positive and of these 16 cases, only 2 cases were positive in the AFB stain or culture study. However, all 18 cases showed the histopathology findings of chronic granulomatous inflammation that was compatible with tuberculosis. Based on the clinical findings, the sensitivity, specificity, positive predictability, and negative predictability of the automated TB-PCR study were 88.9%, 100%, 100%, and 92.3% respectively.Conclusion: An automated TB-PCR assay is an important diagnostic tool for diagnosing tuberculosis in fresh tissue samples. (Tuberc Respir Dis 2006; 61: 54-59)

keywords
Fresh tissue sample, Automated tuberculosis polymerase chain reaction(TB-PCR).

Reference

1.

Field S, (2004) Intestinal and peritoneal tuberculosis, Lippincott Williams & Wilkins

2.

Singh V, (1996) Clinicocolonoscopic profile of colonic tuberculosis,

3.

World Health Organization. , (2004) Global Tuberculosis Control: surveillance, planning, financing: WHO report, World Health Organization

4.

Korea Center for Disease Control & Prevention, (2004) 2004 Communicable Diseases Statistical Yearbook., Korea Center for Disease Control & Prevention

5.

Kim CS, (1997) The usefulness of PCR in AFB smear negative patients on admission.,

6.

Mo EK, (1998) The clinical utility of polymerase chain Reaction in the bronchoalveolar lavage fluid for the detection of Mycobacteria.,

7.

Piersimoni C,, (2003) Relevance of commercial amplification methods for direct detection of Mycobacterium tuberculosis complex in clinical samples.,

8.

Park MH, (1996) The diagnostic value of bronchoalveolar lavage fluid microscopic study and PCR in pulmonary tuberculosis.,

9.

Lee JS, (2005) Clinical utility of polymerase chain reaction for the differentiation of nontuberculous mycobacteria in patients with acid-fast bacilli smear-positive specimens.,

10.

Yu CM, (2004) Usefulness of PCR test for M. tuberculosis for the differentiation of pulmonary tuberculosis and nontuberculous mycobacterial lung disease in patients with smear-positive sputum.,

11.

Lee JG, (2001) Clinical utility of bronchial washing PCR for IS6110 and amplicor for the rapid dignosis of active pulmonary tuberculosis in smear negative patients.,

12.

Rajahti I,, (1998) Detection of Mycobacterium tuberculosis complex by the automated Roche Cobas Amplicor Mycobacterium tuberculosis test.,

13.

Reischl U,, (1998) Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and non respiratory specimens.,

14.

Park DK, (2002) Clinical significance of total colonoscopy in patients with active pulmonary tuberculosis without gastrointestinal symptoms.,

15.

Driscoll JR,, (2005) Genomic analysis of the Mycobacterium tuberculosis complex applications to laboratory diagnosis and genotyping.,

16.

Rimek D,, (2002) Performance of an IS6110-based PCR assay and the COBAS AMPLICOR MTB PCR system for detection of Mycobacterium tuberculosis complex DNA in human lymph node samples.,

17.

Fidler HM, McFadden J. Mycobacterium tuberculosis DNA in tissue affected by sarcoidosis. BMJ 1993,

18.

Singh KK,, (2000) Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy.,

19.

Ko KY, (2004) A case of retroperitoneal tuberculous lymphadenopathy,

20.

Kim SE, (2004) Superior vena caval syndrome due to tuberculous lymphadenitis.,

21.

Kim KM, (1997) Pathologic diagnosis of intestinal tuberculosis in endoscopic biopsied material.,

22.

Lee YJ, (2004) The usefulness of colonoscopic biopsy in the diagnosis of intestinal tuberculosis and pattern of concomitant extra-intestinal tuberculosis.,

23.

Peter JB, (amplifyingouroptions.revinfectdis1991) The polymerase chain reaction: amplifying our options,

24.

Persing DH. Polymerase chain reactions, trenches to benches. J Clin Microbiol 1991,

25.

(serodiagnosisandmolecularbiologicapproach.tubercrespirdis1992) Shin WS. Diagnosis of tuberculosis,

Tuberculosis & Respiratory Diseases

e-Submission OA Policy