The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury

Background: Oxidative stress may play an important role in the pathogenesis of endotoxin-induced acute lung injury (ALI). This study evaluated the therapeutic effect of α-lipoic acid, a nonenzymatic antioxidant, in a rat model of lipopolysaccharide (LPS) induced ALI. Materials and Methods: ALI was induced in Sprague-Dawley rats by instilling LPS (E.coli, 3mg/Kg) into the trachea. The rats were classified into the control, control+α-lipoic acid, LPS, and LPS+α-lipoic acid groups.The lung lavage neutrophil count, cytokine-induced neutrophil chemoattractant (CINC), lung myeloperoxidase (MPO), and cytokine concentrations (TNF-α, IL-1ß, IL-6 and IL-10) were measured at 2 h and 6 h after LPS administration. Results: The total cell and neutrophil counts of the LPS+α-lipoic acid groups were significantly lower than the LPS groups. The protein concentration in the BAL fluid was similar in the LPS groups and LPS+α-lipoic acid groups. The TNF-α, IL-1ß, and IL-6 concentrations in the BAL fluid were not decreased by the α-lipoic acid treatment in the LPS treated rats. Conclusions: Although α-lipoic acid decreased the level of LPS-induced neutrophil infiltration into the lung, it could not attenuate the LPS-induced ALI at the dose administered in this study. (Tuberc Respir Dis 2007; 62: 105-112)

keywords: α-lipoic acid, oxidative stress, acute lung injury, antioxidant., α-lipoic acid, oxidative stress, acute lung injury, antioxidant.

Reference

(2001) Effects of heat pretreatment on histopathology and surfactant in endotoxin- induced acute lung injury,

(2003) Early alterations in neutrophil activation are associated with outcome in acute lung injury,

(2000) Neutrophils as early immunologic effectors in hemorrhage- or endotoxemia-induced acute lung injury,

(2000) Neutrophil recruitment and increased permeability during acute lung injury induced by lipopolysaccharide,

Tissue destruction by neutrophils,

(2003) Oxidative stress and acute lung injury,

(1998) alpha-Lipoic acid: a metabolic antioxidant which regulates NF-kappa B signal transduction and protects against oxidative injury,

(1995) The free radical scavenger, alpha-lipoic acid, protects against cerebral ischemia- reperfusion injury in gerbils,

(2002) Oxidative stress and diabetic neuropathy: pathophysiological mechanisms and treatment perspectives,

10.

(2003) The sensory symptoms of diabetic polyneuropathy are improved with alpha-lipoic acid: the SYDNEY trial,

11.

(2003) Prophylaxis against lipopolysaccharide- induced liver injuries by lipoic acid in rats,

12.

(1996) In vivo antioxidant treatment suppresses nuclear factor-kappa B activation and neutrophilic lung inflammation,

13.

(2003) Vitamin E reduces transendothelial migration of neutrophils and prevents lung injury in endotoxin-induced airway inflammation,

14.

(1998) alpha-Tocopherol enrichment of monocytes decreases agonist-induced adhesion to human endothelial cells,

15.

(2000) Protective effects of N-acetylcysteine and rutin on the lipid peroxidation of the lung epithelium during the adult respiratory distress syndrome,

16.

(1995) alpha-Lipoic acid as a biological antioxidant,

17.

(2002) Antioxidant and prooxidant activities of alpha-lipoic acid and dihydrolipoic acid,

18.

(2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes,

19.

(2004) Regulation of LPS- mediated inflammation in vivo and in vitro by the thiol antioxidant Nacystelyn,

20.

(1996) Superoxide and hydrogen peroxide induce CD18-mediated adhesion in the postischemic heart,

21.

(2001) Effect of vitamin E modified cellulose membrane on human lymphocyte and granulocyte CD11b/CD18 adhesion molecule expression during hemodialysis,

22.

(2002) Pharmacological analysis for mechanisms of GPI-80 release from tumour necrosis factor-alpha-stimulated human neutrophils,

23.

(1999) Treatment of diabetic polyneuropathy with the antioxidant thioctic acid (alpha-lipoic acid): a two year multicenter randomized double-blind placebo-controlled trial (ALADIN II),

24.

(2005) Mechanisms of antioxidant and pro-oxidant effects of alpha-lipoic acid in the diabetic and nondiabetic kidney,

25.

(1995) Inflammatory cytokines in the BAL of patients with ARDS: persistent elevation over time predicts poor outcome,

26.

(1996) Inflammatory cytokines in patients with persistence of the acute respiratory distress syndrome,

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Article Contents

Vol.62 No.2

The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury

Abstract

Reference

Tuberculosis & Respiratory Diseases