바로가기메뉴

본문 바로가기 주메뉴 바로가기

Tuberculosis & Respiratory Diseases

Article Contents

Prev Next
e-Submission

Vol.65 No.6
Citation Share

Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2008, v.65 no.6, pp.464-470



& (2008). Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats. Tuberculosis & Respiratory Diseases, 65(6), 464-470.
  • Downloaded
  • Viewed
PDF Download

Abstract

Background: This study examined the role of neutrophilc oxidative stress in an O2-induced acute lung injury (ALI).Methods: For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage.Forty-eight hours after O2 breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed Results: Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The O2-induced neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase A2 (cPLA2) activity in the lung, histological changes and BAL cytospin morphology. Conclusion: In part, ALI-caused by oxygen is affected by neutrophils especially by the generation of free radicals

keywords
Acute lung injury, O2 toxicity, Neutrophils

Reference

1.

1. Hay WW Jr, Bell EF. Oxygen therapy, oxygen toxicity, and STOP-ROP trial. Pediatrics 2000;105:424-5.

2.

2. Crapo JD, Barry BE, Foscue HA, Shelburne J. Structural and biochemical changes in rat lungs occurring during exposures to lethal and adaptive doses of oxygen. Am Rev Respir Dis 1980;122:123-43.

3.

3. Chabot F, Mitchell JA, Gutteridge JM, Evans TW. Reactive oxygen species in acute lung injury. Eur Respir J 1998;11:745-57.

4.

4. Guidot DM, Repine JE, Kitlowski AD, Flores SC, Nelson SK, Wright RM, et al. Mitochondrial respiration scavenges extramitochondrial superoxide anion via a nonenzymatic mechanism. J Clin Invest 1995;96:1131-6.

5.

5. Ware LB, Matthay MA. The acute respiratory distress syndrome. N Engl J Med 2000;342:1334-49.

6.

6. Brown RE, Jarvis KL, Hyland KJ. Protein measurement using bicinchoninic acid: elimination of interfering substances. Anal Biochem 1989;180:136-9.

7.

7. Goldblum SE, Wu KM, Jay M. Lung myeloperoxidase as a measure of pulmonary leukostasis in rabbits. J Appl Physiol 1985;59:1978-85.

8.

8. Carraway MS, Piantadosi CA, Jenkinson CP, Huang YC. Differential expression of arginase and iNOS in the lung in sepsis. Exp Lung Res 1998;24:253-68.

9.

9. Katsumata M, Gupta C, Goldman AS. A rapid assay for activity of phospholipase A2 using radioactive substrate. Anal Biochem 1986;154:676-81.

10.

10. Capellier G, Maupoil V, Boussat S, Laurent E, Neidhardt A. Oxygen toxicity and tolerance. Minerva Anestesiol 1999;65:388-92.

11.

11. Torbati D, Tan GH, Smith S, Frazier KS, Gelvez J, Fakioglu H, et al. Multiple-organ effect of normobaric hyperoxia in neonatal rats. J Crit Care 2006;21:85-93.

12.

12. Roman K, Vladimíra M, Alexander C. Highly specific, simple and rapid method for the determination of malondialdehyde in blood using high-performance liquid chromatography. Clin Chem Lab Med 2002;40:1032-5.

13.

13. Sukhotnik I, Coran AG, Greenblatt R, Brod V, Mogilner J, Shiloni E, et al. Effect of 100% oxygen on E-selectin expression, recruitment of neutrophils and enterocyte apoptosis following intestinal ischemia-reperfusion in a rat. Pediatr Surg Int 2008;24:29-35.

14.

14. Shvedova AA, Kisin ER, Murray AR, Kommineni C, Castranova V, Fadeel B, et al. Increased accumulation of neutrophils and decreased fibrosis in the lung of NADPH oxidase-deficient C57BL/6 mice exposed to carbon nanotubes. Toxicol Appl Pharmacol 2008;231:235-40.

15.

15. Shasby DM, Fox RB, Harada RN, Repine JE. Reduction of the edema of acute hyperoxic lung injury by granulocyte depletion. J Appl Physiol 1982;52:1237-44.

16.

16. Hybertson BM, Lee YM, Repine JE. Phagocytes and acute lung injury: dual roles for interleukin-1. Ann N Y Acad Sci 1997;832:266-73.

17.

17. Lin Y, Jamieson D. Role of neutrophils in hyperbaric and normobaric oxygen toxicity. Pathophysiology 1995;2:9-16.

18.

18. Prudhomme JB, Ware LB. Acute lung injury and acute respiratory distress syndrome: mechanisms and potential new therapies. Drug Discov Today Dis Mech 2004;1:123-8.

19.

19. Lee YM, Hybertson BM, Terada LS, Repine AJ, Cho HG, Repine JE. Mepacrine decreases lung leak in rats given interleukin-1 intratracheally. Am J Respir Crit Care Med 1997;155:1624-8.

20.

20. Guidot DM. Endotoxin treatment increases lung mitochondrial scavenging of extramitochondrial superoxide in hyperoxia-exposed rats. Arch Biochem Biophys 1996;326:266-70.

21.

21. Boyer CS, Bannenberg GL, Neve EP, Ryrfeldt A, Moldéus P. Evidence for the activation of the signal-responsive phospholipase A2 by exogenous hydrogen peroxide. Biochem Pharmacol 1995;50:753-61.

Tuberculosis & Respiratory Diseases

e-Submission OA Policy