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Tuberculosis & Respiratory Diseases

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Vol.65 No.6
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Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-κB, TGF-β and Fibronectin in WI-26 VA4 Epithelial Cells

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2008, v.65 no.6, pp.504-511








& (2008). Particulate Matter 10 from Asian Dust Storms Induces the Expression of Reactive Oxygen Species, NF-κB, TGF-β and Fibronectin in WI-26 VA4 Epithelial Cells. Tuberculosis & Respiratory Diseases, 65(6), 504-511.
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Abstract

Background: Particulate matter may be toxic to human tissue. Ambient air particulate matter ≤10μm in aerodynamic size (PM10), which changes under different environmental conditions, is a complex mixture of organic and inorganic compounds. The Asian dust event caused by meteorological phenomena can also spread unique particulate matter in affected areas. We evaluated production of ROS, TGF-β, fibronectin, and NFκB by exposing normal epithelial cells to Asian dust particulate matter.Methods: Bronchial epithelial cells were exposed to 0, 50, 100μg/ml of a suspension of PM10 for 24 h. ROS were detected by measurement of DCF release from DCF-DA by FACScan. TGF-β, fibronectin, and NFκB were detected by western blotting. Results: PM10 exposure increased the expression of TGF-β, fibronectin, and NFκB. ROS production and TGF-βlevels were significantly higher with 50 or 100μg/ml PM10. Fibronectin and NFκB production were significantly higher after 100μg/ml of PM10. Conclusion: PM10 from Asian dust particles might have fibrotic potential in bronchial epithelial cells via ROS induction after PM10 exposure

keywords
Particulate matter, Asian dust, Reactive oxygen species, Pulmonary fibrosis

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