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Tuberculosis & Respiratory Diseases

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Vol.70 No.6
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Effect of Apocynin on Acute Lung Injury in Rats Given Interleukin-1α Intratracheally

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2011, v.70 no.6, pp.482-489
(Catholic University of Daegu)
(2011). Effect of Apocynin on Acute Lung Injury in Rats Given Interleukin-1α Intratracheally. Tuberculosis & Respiratory Diseases, 70(6), 482-489.
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Abstract

Background: Based on the assertion that apocynin diminishes acute lung injury (ALI) by inhibition of NADPH oxidase, the effect of apocynin was tested in interleukin-1α (IL-1)-induced ALI in rats. Methods: IL-1 was insufflated into the trachea of Sprague-Dawley rats to induce ALI, and apocynin (8 mg/kg) was given intravenously for inhibition of NADPH oxidase. In addition, we determined whether apocynin inhibited generation of superoxide anions from isolated human neutrophils. Five hours after IL-1 instillation, lung injury parameters, expression of cytosolic phospholipase A2 (cPLA2) by cells from bronchoalveolar lavage (BAL), an index of oxidative stress in lung tissues (γ-glutamyltranspeptidase, activity), and ultrastructure of alveolar type II (AT II) cells were evaluated. Results: Apocynin decreased the generation of free radicals from phorbol myristate (PMA)-activated neutrophils in vitro, but did not ameliorate ALI. IL-1 induced enhancement of the expression of cPLA2 on neutrophils was not altered by apocynin. Conclusion: Apocynin induced suppression of the generation of superoxide anions from neutrophils by inhibition of NADPH oxidase does not attenuate IL-1-induced ALI in rats.

keywords
apocynin, NADPH Oxidase, Acute Lung Injury, Neutrophils, apocynin, NADPH Oxidase, Acute Lung Injury, Neutrophils

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