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Vol.77 No.3
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Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2014, v.77 no.3, pp.116-123






& (2014). Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema. Tuberculosis & Respiratory Diseases, 77(3), 116-123.
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Abstract

Background: Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repairemphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describethe cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. Thedistributions were also compared to the distributions in control mice without emphysema. Methods: We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCsinjection. Results: The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema. Conclusion: In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.

keywords
Mesenchymal Stromal Cells, Emphysema, Cell Tracking, Injections, Intravenous

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