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Tuberculosis & Respiratory Diseases

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Vol.77 No.5
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Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2014, v.77 no.5, pp.203-208










& (2014). Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells. Tuberculosis & Respiratory Diseases, 77(5), 203-208.
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Abstract

Background: In this study, we investigated whether lobetyolin, lobetyol, and methyllinoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion,production, and gene expression from airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein productionand secretion were measured by reverse transcription polymerase chain reactionand enzyme-linked immunosorbent assay, respectively. Results: Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression ofMUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5ACmucin production. However, lobetyol and methyl linoleate inhibited the production ofMUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-inducedMUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreasedthe MUC5AC mucin secretion. Conclusion: These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin bydirectly acting on the airway epithelial cells.

keywords
Mucins, MUC5AC Protein, Human, Methyl Linoleate

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