Roles of Inflammatory Biomarkers in Exhaled Breath Condensates in Respiratory Clinical Fields
Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2024, v.87 no.1, pp.65-79
https://doi.org/10.4046/trd.2023.0028
Hye Jung Park, M.D., Ph.D. (Departments of 1Internal Medicine and Yonsei University College of Medicine, Seoul, Korea)
Yong Jun Choi, M.D. (1Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul)
Min Jae Lee, M.D. (Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul)
Min Kwang Byun, M.D., Ph.D. (Department of Internal Medicine, Gangnam Severance Hospital)
Sangho Park, B.S. (1Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul)
Jimyung Park, M.D., Ph.D. (Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Seoul National University Hospital, Seoul)
Dongil Park, M.D., Ph.D. (Department of Internal Medicine, Chungnam National University College of Medicine, Daejeon)
Sang-Hoon Kim, M.D., Ph.D. (Department of Internal Medicine, Nowon Eulji Medical Center, Eulji University School of Medicine, Seoul)
Young Sam Kim, M.D., Ph.D. (Severance Hospital, Younsei University Health System, Yonsei University College of Medicine, Seoul)
Seong Yong Lim, M.D., Ph.D. (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul)
Kwang Ha Yoo, M.D. Ph.D. (Konkuk University School of Medicine)
Ki-Suck Jung, M.D., Ph.D (Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang)
Hye,
J.
P.
M.
P.
, Yong,
J.
C.
M.
, Min,
J.
L.
M.
, Min,
K.
B.
M.
P.
, Sangho,
P.
B.
, Jimyung,
P.
M.
P.
, Dongil,
P.
M.
P.
, Sang-Hoon,
K.
M.
P.
, Young,
S.
K.
M.
P.
, Seong,
Y.
L.
M.
P.
, Kwang,
H.
Y.
M.
P.
, &
Ki-Suck,
J.
M.
P.
(2024). Roles of Inflammatory Biomarkers in Exhaled Breath Condensates in Respiratory Clinical Fields. Tuberculosis & Respiratory Diseases, 87(1), 65-79, https://doi.org/10.4046/trd.2023.0028
Abstract
Background: Exhaled condensates contain inflammatory biomarkers; however, theirroles in the clinical field have been under-investigated.
Methods: We prospectively enrolled subjects admitted to pulmonology clinics. Wecollected exhaled breath condensates (EBC) and analysed the levels of six and 12biomarkers using conventional and multiplex enzyme-linked immunosorbent assay, respectively.
Results: Among the 123 subjects, healthy controls constituted the largest group (81participants; 65.9%), followed by the preserved ratio impaired spirometry group (21patients; 17.1%) and the chronic obstructive pulmonary disease (COPD) group (21patients; 17.1%). In COPD patients, platelet derived growth factor-AA exhibited strongpositive correlations with COPD assessment test (ρ=0.5926, p=0.0423) and COPD-specificversion of St. George’s Respiratory Questionnaire (SGRQ-C) score (total, ρ=0.6725,p=0.0166; activity, ρ=0.7176, p=0.0086; and impacts, ρ=0.6151, p=0.0333). GranzymeB showed strong positive correlations with SGRQ-C score (symptoms, ρ=0.6078,p=0.0360; and impacts, ρ=0.6007, p=0.0389). Interleukin 6 exhibited a strong positivecorrelation with SGRQ-C score (activity, ρ=0.4671, p=0.0378). The absolute serum eosinophiland basophil counts showed positive correlations with pro-collagen I alpha 1(ρ=0.6735, p=0.0164 and ρ=0.6295, p=0.0283, respectively). In healthy subjects, forcedexpiratory volume in 1 second (FEV1)/forced vital capacity demonstrated significantcorrelation with CC chemokine ligand 3 (CCL3)/macrophage inflammatory protein 1alpha (ρ=0.3897 and p=0.0068). FEV1 exhibited significant correlation with CCL11/eotaxin(ρ=0.4445 and p=0.0017).
Conclusion: Inflammatory biomarkers in EBC might be useful to predict quality of lifeconcerning respiratory symptoms and serologic markers. Further studies are needed.
- keywords
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Exhaled Condensates,
Chronic Obstructive Pulmonary Disease,
Lung Function Test,
Eosinophils