open access
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open access
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ISSN : 0376-4672
Objectives: Periodontitis is multifactorial disease mainly caused by microbial community. Recently, some research has been conducted to find other possible risk factors including stress hormones related to periodontitis. Psychological stress can affect the periodontal health by a variety of biological mechanisms. This study compared the stress hormone levels in healthy subjects and patients with periodontal disease using saliva in order to investigate the association between periodontitis and stress. Methods: The human saliva was collected from 38 periodontally healthy individuals and 34 patients with chronic periodontitis under Institutional Review Board. Their age was 20-60 years (40.3 10.45). From these samples, determination of salivary levels of cortisol and Dehydroepiandrosterone (DHEA) performed by enzyme immunoassay kit (Salimetrics Europe, Suffolk, UK). The independent t-test and Mann-Whitney test for trend was applied using IBM SPSS statistics version 12.0 Program to analyze statistically significant differences. Results: Salivary cortisol levels of periodontitis patients were higher than those levels of healthy subjects (P < 0.001), while salivary DHEA levels of periodontitis patients were not significantly different (P = 0.431). Salivary cortisol/DHEA ratio of periodontitis patients was higher than those levels of healthy subjects (P < 0.001). Conclusions: Our study demonstrates the high levels of cortisol concentrations and cortisol/DHEA ratio in saliva of periodontitis patients than those of healthy subjects. Since cortisol levels and cortisol/DHEA ratio can be significant factors related to the severity of periodontal disease, our study would be helpful for early diagnosis and treatment of periodontal disease.
1. Mesa, F., et al. Catecholamine metabolites in urine, as chronic stress biomarkers, are associated with higher risk of chronic periodontitis in adults. J Periodontol 2014; 85(12): 1755-62.
2. Page, R.C. and K.S. Kornman. The pathogenesis of human periodontitis: an introduction. Periodontol 2000 1997; 14: 9-11.
3. Cronin, A.J., N. Claffey, and L.F. Stassen. Who is at risk? Periodontal disease risk analysis made accessible for the general dental practitioner. Br Dent J 2008; 205(3): 131-7.
4. Lazarus, R.S. From psychological stress to the emotions: a history of changing outlooks. Annu Rev Psychol 1993; 44: 1-21.
5. Chrousos, G.P. The hypothalamic-pituitary-adrenal axis and immune-mediated inflammation. N Engl J Med 1995; 332(20): 1351-62.
6. Johannsen, A., N. Bjurshammar, and A. Gustafsson. The influence of academic stress on gingival inflammation. Int J Dent Hyg 2010; 8(1): 22-7.
7. Michael, A., Jenaway, A., Paykel, E.S., Herbert, J. Altered salivary dehydroepiandrosterone levels in major depression in adults. Biol. Psychiatry 2000;48: 989-95.
8. Goodyer, I.M., Herbert, J., Tamplin, A. Psychoendocrine antecedents of persistent firstepisode major depression in adolescents: a community-based longitudinal enquiry. Psychol. Med 2003; 33: 601-10.
9. Mandel, I.D. Sialochemistry in diseases and clinical situations affecting salivary glands. Crit Rev Clin Lab Sci 1980; 12(4): 321-66.
10. Mori, E., et al. Comparing older and younger Japanese primiparae: fatigue, depression and biomarkers of stress. Int J Nurs Pract 2015; 21Suppl 1: 10-20.
11. LeResche, L. and S.F. Dworkin. The role of stress in inflammatory disease, including periodontal disease: review of concepts and current findings. Periodontol 2000 2002; 30: 91-103.
12. Roberts, A., et al. Stress and the periodontal diseases: effects of catecholamines on the growth of periodontal bacteria in vitro. Oral Microbiol Immunol 2002; 17(5): 296-303.
13. Roberts, A., et al. Stress and the periodontal diseases: growth responses of periodontal bacteria to Escherichia coli stress-associated autoinducer and exogenous Fe. Oral Microbiol Immunol 2005;20(3): 147-53.
14. Kurita-Ochiai T, Jia R, et al. Periodontal Disease-Induced Atherosclerosis and Oxidative Stress. Antioxidants (Basel). 2015; 4(3): 577-90.
15. Yamaguchi, M., Y. Tahara, and S. Kosaka. Influence of concentration of fragrances on salivary alpha-amylase. Int J Cosmet Sci 2009; 31(5): 391-5.
16. Armitage, G.C. Development of a classification system for periodontal diseases and conditions. Ann Periodontol 1999; 4(1): 1-6.
17. Murugesh J, Annigeri R.G, et al. Effect of yogurt and pH equivalent lemon juice on salivary flow rate in healthy volunteers - An experimental crossover study. Interv Med Appl Sci. 2015; 7(4): 147-51.
18. Merimee, T.J. and S.E. Fineberg. Homeostasis during fasting. II. Hormone substrate differences between men and women. J Clin Endocrinol Metab 1973; 37(5): 698-702.
19. Windham, G.C., et al. Cigarette smoking and effects on hormone function in premenopausal women. Environ Health Perspect 2005; 113(10):1285-90.
20. Miller, C.S., et al. Current developments in salivary diagnostics. Biomark Med 2010; 4(1): 171-89.
21. Chiappin, S., et al. Saliva specimen: a new laboratory tool for diagnostic and basic investigation. Clin Chim Acta 2007; 383(1-2): 30-40.
22. Chicharro, J.L., et al. Saliva composition and exercise. Sports Med 1998; 26(1): 17-27.
23. de Almeida Pdel, V., et al. Saliva composition and functions: a comprehensive review. J Contemp Dent Pract 2008; 9(3): 72-80.
24. Clements, A.D. and C.R. Parker. The relationship between salivary cortisol concentrations in ;frozen versus mailed samples. Psychoneuroendocrinology 1998; 23(6): 613-6.
25. Tóthová, L., Kamodyová, N., Cervenka, T., Celec P. Salivary markers of oxidative stress in oral diseases. Front Cell Infect Microbiol. 2015; 5: 73.
26. da Silva, A.M., H.N. Newman, and D.A. Oakley. Psychosocial factors in inflammatory periodontal diseases. A review. J Clin Periodontol 1995; 22(7):516-26.
27. Mannem, S. and V.K. Chava. The effect of stress on periodontitis: A clinicobiochemical study. J Indian Soc Periodontol 2012; 16(3): 365-9.
28. Croucher, R., et al. The relationship between lifeevents and periodontitis. A case-control study. J Clin Periodontol 1997; 24(1): 39-43.
29. Ebrecht, M., et al. Perceived stress and cortisol levels predict speed of wound healing in healthy male adults. Psychoneuroendocrinology 2004;29(6): 798-809.
30. Zukowski, Ł., Mysliwiec, J., Górska, M. Diagnostics of hypercortisolism - comparison between the clinical usefulness of salivary and serum cortisol measurements. Endokrynol Pol. 2013; 64(4): 263-7.
31. Warren, K.R., et al. Role of chronic stress and depression in periodontal diseases. Periodontol 20002014; 64(1): 127-38.
32. Johannsen, A., et al. Dental plaque, gingival inflammation, and elevated levels of interleukin-6and cortisol in gingival crevicular fluid from women with stress-related depression and exhaustion. Periodontol 2006 7;7(8): 1403-9.
33. Powell, J., et al. Salivary and serum cortisol levels during recovery from intense exercise and prolonged, moderate exercise. Biol Sport 2015;32(2): 91-5.
34. Rosania, A.E., et al. Stress, depression, cortisol, and periodontal disease. J Periodontol 2009; 80(2):260-6.
35. Ishisaka, A., et al. Association of cortisol and dehydroepiandrosterone sulphate levels in serum with periodontal status in older Japanese adults. J Clin Periodontol 2008; 35(10): 853-61.
36. Christeff, N., E.A. Nunez, and M.L. Gougeon. Changes in cortisol/DHEA ratio in HIV-infected men are related to immunological and metabolic perturbations leading to malnutrition and lipodystrophy. Ann N Y Acad Sci 2000; 917: 962-70.
37. Shirotsuki, K., et al. Salivary cortisol and DHEA reactivity to psychosocial stress in socially anxious males. Int J Psychophysiol 2009; 72(2): 198-203.