- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Log In/Sign Up
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2002, v.23 no.4, pp.705-713
&
(2002). Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan. Journal of Korean Medicine, 23(4), 705-713.
- Downloaded
- Viewed
Abstract
Objectives : Peroxynitrite (ONOO-), formed from the reaction of superoxide (·O2-) and nitric oxide (·NO), is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in diseases such as aging process, Alzheimer's disease, rheumatoid arthritis, cancer and arteriosclerosis. Due to the lack of endogenous enzymes responsible for ONOO- inactivation, developing a specific ONOO- scavenger is of considerable importance. The aim of this study was to evaluate ONOO- scavenging activity and its mechanism of Cheongahwan (CAH). Methods : The ONOO- scavenging activity on CAH was assayed by measuring oxidized dihydrorhodamine 123 (DHR 123) by fluorescence. The scavenging efficacy was expressed as IC50 showing the concentration of each sample that requires to cause 50% inhibition of DHR 123 oxidation. In a separate study, the protective effect of CAH on ONOO--induced nitration of bovine serum albumin (BSA) was investigated using immunoassay with a monoclonal anti-nitryrosine antibody, and a horseradish peroxidase- conjugated anti-mouse secondary antibody from sheep.Results : CAH showed the potent scavenging activities of ONOO-, NO and ·O2. The data demonstrated that CAH led to decreased ONOO- mediated nitration of tyrosine through electron donation. CAH showed significant inhibition on nitration of bovine serum albumin by ONOO- in a dose-dependent manner.Conclusions : CAH can be developed as an effective peroxynitrite scavenger for the prevention of the ONOO- involved diseases.
- keywords
- Cheongahwan, scavenging activity, peroxynitrite, nitric oxide, superoxide, Cheongahwan, scavenging activity, peroxynitrite, nitric oxide, superoxide
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Effect of Phaseoli Angularis Seman on Biochemical and Histological Changes of Rats Fed High Fat Diet
- The Clinical Efficacy of Chingpyesagan-tang on Acute Stroke
- A Study on the Severity Scoring Systems of Atopic Dermatitis ; Comparision, Analysis and Establisment
- Observation of Micromorphological Characteristics of Acupuncture Needle Tip Using SEM
- Antipyretic Effect of Rhizoma Cimicifugae in a Rat Model of LPS-Induced Fever
- Inhibitory Effect of Methanolic Extract from Radix Trichosanthis on Melanin Synthesis
- Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan
- Effects of Ramulus mori Extract on Obesity and Lipid Metabolism in High Fat Diet Rats
- A Clinical Study on the Factors that Influence Functional Evaluation of Stroke
- Survival Probability of the Patients with Cerebral Vascular Disease Who Visited an Oriental Hospital
- Immunohistochemical Study on the Effects of Cortex Acanthopanacis Senticosi on the Expression of Calpain in Collagen-Induced Arthritis Rats
- Effects of Silsosangami-extract on Endotoxin-induced Experimental Thrombosis in Rats
- Protective Effect of Bupleuri Radix on Hypoxia Reperfusion Induced by PC12 Cell Damage and Global Ischemia in Gerbil
- The Effect of Woohwangcheongsim-won for Delayed Neuronal Death in OGD (Oxygen-Glucose Deprivation) Model
- Effect & Mechanism of Omija-tang on Oxidative Stress-Induced Death of H9c2 Cardiomyoblast Cell
- Clinical Effects of Chuongsangboha-tang in Asthmatic Patients
- Neuroprotective Effect of Hwangryunhaedok-tang on the Brain Ischemia Induced by Four-Vessel Occlusion in Rats
- The Effects of Sasammaickmoondong-tang against Colonic Mucosal Lesions
- The Characteristics of Perspiration According to Sasang Constitution
- Two Cases of Combination Therapy of Acupuncture, Herbal medication and Speech Therapy for Aphasic Stroke Patients
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.