- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2002, v.23 no.4, pp.705-713
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(2002). Peroxynitrite Scavenging Activity and its Mechanism of Cheonga-hwan. Journal of Korean Medicine, 23(4), 705-713.
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Abstract
Objectives : Peroxynitrite (ONOO-), formed from the reaction of superoxide (·O2-) and nitric oxide (·NO), is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in diseases such as aging process, Alzheimer's disease, rheumatoid arthritis, cancer and arteriosclerosis. Due to the lack of endogenous enzymes responsible for ONOO- inactivation, developing a specific ONOO- scavenger is of considerable importance. The aim of this study was to evaluate ONOO- scavenging activity and its mechanism of Cheongahwan (CAH). Methods : The ONOO- scavenging activity on CAH was assayed by measuring oxidized dihydrorhodamine 123 (DHR 123) by fluorescence. The scavenging efficacy was expressed as IC50 showing the concentration of each sample that requires to cause 50% inhibition of DHR 123 oxidation. In a separate study, the protective effect of CAH on ONOO--induced nitration of bovine serum albumin (BSA) was investigated using immunoassay with a monoclonal anti-nitryrosine antibody, and a horseradish peroxidase- conjugated anti-mouse secondary antibody from sheep.Results : CAH showed the potent scavenging activities of ONOO-, NO and ·O2. The data demonstrated that CAH led to decreased ONOO- mediated nitration of tyrosine through electron donation. CAH showed significant inhibition on nitration of bovine serum albumin by ONOO- in a dose-dependent manner.Conclusions : CAH can be developed as an effective peroxynitrite scavenger for the prevention of the ONOO- involved diseases.
- keywords
- Cheongahwan, scavenging activity, peroxynitrite, nitric oxide, superoxide, Cheongahwan, scavenging activity, peroxynitrite, nitric oxide, superoxide
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