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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Effects in Metabolism and Adipocyte Inflammation Induced by the Complex Herbal Medicine composed of Cornus officinalis, Dioscorea rhizoma, Aurantii fructus, Mori folium in Obese Type 2 Diabetes Mouse Model

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2012, v.33 no.3, pp.184-199






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Abstract

Objectives: There is a steady increase in the prevalence of obesity worldwide and obesity is often accompanied by inflammation. Although much emphasis has been placed on the adipose tissue inflammation in obesity, a study with herbal medicine is few. This study aimed to investigate the antidiabetic and anti-inflammatory effect of a complex herbal medicine (CHM) composed of Cornus officinalis, Dioscorea rhizoma, Aurantii fructus, and Mori Foliumon on obese type 2 diabetes mice. Methods: Type 2 diabetes mellitus and obesity were induced by Surwit's high fat, high sucrose diet for 8 weeks. Mice were divided into ND (normal diet, n=10), HFD (high fat and high sucrose diet, n=10), CHM (high fat and high sucrose diet with complex herbal medicine, n=10) and Met (high fat and high sucrose diet with metformin, n=10) groups. The body weight, fructosamine and OGTT (oral glucose tolerance test) were measured. After 8 weeks the blood samples of all mice were taken from the heart, and lipid profiles were measured. Epididymal fat pad, histological size of the adipocyte tissue and liver weights were measured. Inflammatory markers such as leptin and adipocyte tissue macrophage were measured to evaluate the effect of CHM on adipocyte tissue inflammation. Results: Compared with the HFD group, there was an improvement in OGTT and epididymal fat decreased in the CHM group. White adipocyte size and adipocyte tissue macrophage decreased in CHM group. Conclusions: These results suggest that CHM has antidiabetic and anti-inflammatory effects in high fat, high sucrose diet induced obese mice.

keywords
Cornus officinalis, Dioscorea rhizoma, Aurantii fructus, Mori Folium, Adipocyte tissue macrophage, Obesity


Reference

1

1. Gardner D, Shoback D. Greenspan's basic & clinical endocrinology. McGrawHill. 2007:675-6.

2

2. Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-a: direct role in obesity-linked insulin resistance. Science. 1993;259(5091):87-91.

3

3. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW Jr. Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest. 2003;112:1796-808.

4

4. Cancello R, Clement K. Is obesity an inflammatory illness? Role of low-grade inflammation and macrophage infiltration in human white adipose tissue. BJOG. 2006;113(10): 1141-7.

5

5. Yamabe N, Kang KS, Matsuo Y, Tanaka T, Yokozawa T. Identification of antidiabetic effect of iridoid glycosides and low molecular weight polyphenol fractions of Corni Fructus, a constituent of Hachimi-jio-gan, in streptozotocin-induced diabetic rats. Biol Pharm Bull. 2007 Jul;30(7):1289-96.

6

6. Hsu JH, Wu YC, Liu IM, Cheng JT. Dioscorea as the principal herb of Die-Huang-Wan, a widely used herbal mixture in China, for improvement of insulin resistance in fructose-rich chow-fed rats. J Ethnopharmacol. 2007 Jul 25;112(3): 577-84.

7

7. Jung UJ, Lee MK, Jeong KS, Choi MS. The Hyp oglycemic Effects of Hesperidin and Naringin Ar e Partly Mediated by Hepatic Glucose-Regulatin g Enzymes in C57BL/KsJ-db/db Mice. J. Nutr. 2004;134:2499–503.

8

8. Kurowska EM, Manthey JA. Hypolipidemic Effects and Absorption of Citrus Polymethoxylated Flavones in Hamsters with Diet-Induced Hypercholesterolemia. J. Agric. Food Chem. 2004;52:2879-86.

9

9. Oh KS, Ryu SY, Lee S, Seo HW, Oh BK, Kim YS, et al. Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet- induced obese mice. J Ethnopharmacol. 2009; 122(2):216-20.

10

10. Park JM, Bong HY, Jeong HI, Kim YK, Kim JY, Kwon O. Postprandial hypoglycemic effect of mulberry leaf in Goto-Kakizaki rats and counterpart control Wistarrats. Nutr Res Pract. 2009; 3(4):272-8.

11

11. Kimura T, Nakagawa K, Kubota H, Kojima Y, Goto Y, Yamagishi K, et al. Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. J Agric Food Chem. 2007; 55(14):5869-74.

12

12. Willett WC, Dietz WH, Colditz GA. Guidelines for healthy weight. NEJM. 1999;341(6):427-34.

13

13. Knowler WC, Barrett-Connor E, Fowler SE, Hamman RF, Lachin JM, Walker EA, et al. Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin. NEJM. 2002;346(6):393-403.

14

14. Kadowaki T, Wamauchi T, Kubota N, Hara K, Ueki K, Tobe K. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome. J Clin Invest. 2006;116(7):1784-92.

15

15. Manabe IR. Chronic inflammation links cardiovascular metabolic and renal diseases. Circ J. 2011;75(12):2739-48.

16

16. Cinti S, Mitchell G, Barbatelli G, Murano I, Ceresi E, Faloia E, et al. Adipocyte death defines macrophage localization and function in adipose tissue of obese mice and humans. J Lipid Res. 2005;46(11):2347-55.

17

17. Zeyda M, Farmer D, Todoric J, Aszmann O, Speiser M, Gyori G, et al. Human adipose tissue macrophages are of an anti-inflammatory phenotype but capable of excessive pro-inflammatory mediator production. Int J Obes(Lond). 2007;31 (9):1420-8.

18

18. Odegaard JI, Ricardo-Gonzalez RR, Goforth MH, Morel CR, Subramanian V, Mukundan L, et al. Macrophage-specific PPARr controls alternative activation and improves insulin resistance. Nature. 2007;447:1116-20.

19

19. Nissen SE, Wolski K. Effect of rosiglitazone on the risk of myocardial infarction and death from cardiovascular causes. N Engl J Med. 2007;356 (24):2457-71.

20

20. Dormandy JA, Charbonnel B, Eckland DJ, Erdmann E, MassiBenedetti M, Moules IK, et al. Secondary prevention of macrovasular events in patients with type 2 diabetes in the proactive study: A randomised controlled trial. Lancet. 2005;366(9493):1279-89.

21

21. Bunck MC, Diamant M, Eliasson B, Cornér A, Shaginian RM, Heine RJ, et al. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition. Diabetes Care. 2010;33(8):1734-7.

22

22. 전국한의과대학 본초학교수. 본초학. 서울:도서출판 영림사. 2000:351-2, 537-8, 626-7.

23

23. Park JS, Lee BC, Doo HK, Ahn YM, Ahn SY. The Effects of Supungsunki-hwan on High Fat, High Carbohydrate Diet-Induced Obese Type 2 Diabetic Mouse Model. Korean J. Orient. Int. Med. 2009;30(2):257-69.

24

24. Lee BC, Kwon YG, Choi KL, Lee JS, Ahn YM, Ahn SY, et al. The Hypoglycemic Effect of Supungsunkihwan on Impaired Glucose Tolerance & Mild NIDDM Patients. Korean J. Orient. Int. Med. 2001;22(3):285-90.

25

25. Park EY, Ahn SY, Ahn YM, Um JY, Jang HJ, Lee BC. The effects of Supungsunki-hwan Partitioned Prescriptions on Obese Type 2 Diabetes Mouse Model Induced by High Fat, High Carbohydrate Diet. Korean J. Orient. Int. Med. 2011;32(3):387-96.

26

26. Cho HK. Diabetes Mellitus and Disorder of Lipid Metabolism. Endocrinol Metab. 2006;21(2):101-5.

27

27. Song SY, Ahn SY, Ahn YM, Um JY, Jang HJ, Lee BC. Anti-Glycemic and Anti-Lipid Effect of Ethanol Extract of Supungsunkihwan-gagambang in High Fat Diet-induced Obese Mice. J Korean Oriental Med. 2011;32(5):1-11.

28

28. Zeyda M, Stulnig T. Obesity, inflammation, and insulin resistance-a mini-review. Gerontology. 2009;55(4):379-86.

29

29. Loffreda S, Yang SQ, Lin HZ, Karp CL, Brengman ML, Wang DJ, et al. Leptin regulates proinflammatory immune responses. FASEB J. 1998;12:57-65.

30

30. Pingping Li. Functional Heterogeneity of CD11c-positive Adipose Tissue Macrophages in Diet-induced Obese Mice. Journal of biological chemistry. 2010; 285(20):15333-45.

31

31. 허준. 동의보감. 경남하동:동의보감출판사. 2005: 1443-5.

32

32. Heymsfield SB, Greensberg AS, Fujioka K, Dixon RM, Kushner R, Hunt T, et al. Recombinant leptin for weight loss in obese and lean adults: a randomized, controlled, dose-escalation trial. JAMA. 1999;282(16):1568-75.

33

33. Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. NEJM. 1996;334(5):292-5.

34

34. 전국한의과대학 신계내과학교실. 신계내과학. 서울:군자출판사. 2011:91.

35

35. Cancello R, Clement K. Is obesity an inflammatory illness? Role of low-grade inflammation and macropohage infiltration in human white adipose tissue. RCOG. 2006;113: 1141-7.

36

36. Kim OG, Jeong JC. Effects of the Mori folium in Streptozotocin-Induced Diabetic Rats. Korean J. Orient. Int. Med. 2006;27(4):811-9.

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