- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2014, v.35 no.3, pp.60-69
&
(2014). Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods. Journal of Korean Medicine, 35(3), 60-69.
- Downloaded
- Viewed
Abstract
Background: Herbal formulas are generally served as a type of decoction. However, there is no scientific evidence for determining preservation and circulation period of herbal medicine decoctions. Thus, we investigated anti-inflammatory and anti-oxidative effects of the Gwakhyangjeonggi-san decoction according to its preservation period. Methods: Gwakhyangjeonggi-san decoction was stored for 0, 1, 2 or 3 months at room temperature. To evaluate anti-inflammatory effects, enzyme-linked immunosorbent assays (ELISAs) for tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), and nitric oxide (NO) assay were conducted using the culture supernatant from RAW 264.7 cells stimulated with lipopolysaccharide (LPS). The antioxidant activities were studied by measuring free radical scavenging activities on ABTS and DPPH. Results: Gwakhyangjeonggi-san decoction maintained the inhibitory effects on TNF-α, IL-6 and NO productions after up to 2 months of storage in LPS-treated RAW 264.7 cells. No inflammatory response was observed in 3 months of storage. In addition, the scavenging activities on ABTS and DPPH of Gwakhyangjeonggi-san decoction were reduced time-dependently and showed less than 50% inhibition after 3 months of storage. Conclusions: Our results suggest that preservation period of Gwakhyangjeonggi-san decoction is recommended within 2 months after storage.
- keywords
- Gwakhyangjeonggi-san decoction, preservation period, anti-inflammation, anti-oxidation
Reference
1
1. Adessi C, Soto C. Converting a peptide into adrug: strategies to improve stability and bioavailability. Curr Med Chem. 2002;9(9):963-78.
2
2. 한의과대학 방제학교수 공편저. 방제학-개정증보판-. Seoul:Yeongnimsa. 2003:488-90.
3
3. Yu C, Zhu L. Explore of immune mechanism of HUOXIANG ZHENGQI ORAL LIQUOR restrain degranulation of mast cell. Chin Traditional Patent Med. 2002;2:120-1.
4
4. Yu C, Zhu L. Experimental researches on inhibitory effect of Huoxiang Zhengqi Liquid (藿香正气水) on histamine release. Chin J Integrative Med. 2003;9(4):276-80.
5
5. Sun JK, Kim HH, Nam CG, Koo CM. Effects of GwakHyangJungGiSan on the arterial contraction in rabbit. J Korean Oriental Intern. 2003;24(2):260-8.
6
6. Xue X, Huang X, Gao N, Liu E, Ren L. Effects of Huoxiang Zhengqi liquid on the anti-oxidation and expression of EGFR in gastric mucosa of rats with dampness retention syndrome. Chin J Exp Traditional Med Formulae. 2012;18(21):230-4.
7
7. Yuan Y. Study on the effect of Huoxiang Zhengqi liquid on the gastrointestinal tract in rats. J Pract Med Tech. 2007;14(16):2137-8.
8
8. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med. 1999;26:1231-7.
9
9. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnophamacol. 2000;71:109-4.
10
10. Ministry of Food and Drug Safety. Ministry of Food and Drug Safety Notification 2013-253. 2013.
11
11. Ministry of Food and Drug Safety. Ministry of Food and Drug Safety Notification 2013-123. 2013.
12
12. Ministry of Food and Drug Safety. Ministry of Food and Drug Safety Notification 2009-101. 2009.
13
13. Seo CS, Kim JH, Kim SS, Lim SH, Shin HK. Evaluation of shelf-life of Bojungikgi-tang by long-term storage test. Korean J Pharmacogn. 2013;44(2):200-8.
14
14. Seo CS, Kim JH, Lim SH, Shin HK. Establishment of shelf-Life of Ssanghwa-tang by long-term storage test. Korean J Pharmacogn. 2012;43(3):257-64.
15
15. Seo CS, Kim JH, Lim SH, Shin HK. Estimation of shelf-life by long-term storage test of Pyungwi-san. Korean J Oriental Med Prescription. 2011;19(1):183-94.
16
16. Son JY, Shin JW, Son CG. Stability study for herbal drug according to storage conditions and periods. J Korean Oriental Med. 2009;30(2): 127-32.
17
17. Ha H, Shin IS, Lim HS, Jeon WY, Kim JH, Seo CS, et al. Changes in anti-inflammatory effect of Pyungwi-san decoction according to the preservation temperature and period. Formular Sci. 2012;20(2):29-35.
18
18. Kil GJ, Lim DB, Lee YJ. A study on the degraded effect of decocted Yeonkyopaedogsan over a period. Korean J Herbology. 1998;13(1):173-86.
19
19. Kim WG, Choi YB, Lee YJ. A study on the degraded effect of decocted Injinhotang over a period. Koean J Herbology. 1998;13(2):14-8.
20
20. Zhou X, Chan SW, Tseng HL, Deng Y, Hoi PM, Choi PS, et al. Danshensu is the major marker for the antioxidant and vasorelaxation effects of Danshen (Salvia miltiorrhiza)water-extracts produced by different heat water-extractions. Phytomed. 2012;19(14):1263-9.
21
21. Shen B, Truong J, Helliwell R, Govindaraghavan S, Sucher NJ. An in vitro study of neuroprotective properties of traditional Chinese herbal medicines thought to promote healthy ageing and longevity. BMC Complement Altern Med. 2013;13:373.
22
22. Hilmi Y, Abushama MF, Abdalgadir H, Khalid A, Khalid H. A study of antioxidant activity, enzymatic inhibition and in vitro toxicity of selected traditional Sudanese plants with anti-diabetic potential. BMC Complement Altern Med. 2014;14:149.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Review Study on Ryodoraku Diagnosis Study Methods
- Korean Medical Care and Education Activity in Uzbekistan - Focusing on the Korea-Uzbekistan Friendship Hospital of Korean Medicine -
- The Relationship between the Use of Korean and Western Medicine in treating Musculoskeletal Disease
- The Analysis of Contents Relevant to Korean Medicine in Elementary School National Textbooks
- A Review on Korean Medicine and Personalized Medicine: Syndrome-based Personalized Medicine on the Basis of Syndrome Differentiation and Treatment
- Exploratory Structural Analysis on Eight Positions for New Formulations in Jingyuequanshu by Network Analysis
- Comparative Study on Biological Activities of Gwakhyangjeonggi-san Decoction According to the Preservation Periods
- Using Ultrasonography in Korean Medicine to Observe Organs and Diseases, and Evidence of its Use.
- Revision and Evaluation of Korean Outpatient Groups-Korean Medicine
- Survey of Demand for Medical Devices in Korean Medicine
- A Review Study on Ryodoraku Diagnosis and Evaluation of Low Back Pain
- Review of International Programs of Chinese Medicine University in China
- Study on the Application of Established Korean Medical Classics in the Korean Pharmaceutical Affairs Act
- A Research of characteristics of left/right pulse wave and blood vessel using Korean medicine pulse diagnosis
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.