- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2017, v.38 no.4, pp.1-10
(Department of Periodontics, College of Medicine, The Catholic University)
(Ethnobotanical Database of Bangladesh)
(Ethnobotanical Database of Bangladesh)
Salah,
U.
, &
(2017). The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva. Journal of Korean Medicine, 38(4), 1-10.
- Downloaded
- Viewed
Abstract
Objectives: The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.
- keywords
- cell differentiation, herbal medicine, Pongamia, stem cells
Reference
1
1. Ha DH, Pathak S, Yong CS, Kim JO, Jeong JH, Park JB. Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus. Sci Rep. 2016;6:34910.
2
2. Maumus M, Jorgensen C, Noel D. Mesenchymal stem cells in regenerative medicine applied to rheumatic diseases: role of secretome and exosomes. Biochimie. 2013;95:2229-2234.
3
3. Jeong SH, Lee JE, Jin SH, Ko Y, Park JB. Effects of Asiasari radix on the morphology and viability of mesenchymal stem cells derived from the gingiva. Mol Med Rep. 2014;10:3315-3319.
4
4. Jeong SH, Kim BB, Lee JE, Ko Y, Park JB. Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells. Mol Med Rep. 2015;12:1556-1560.
5
5. Jeong SH, Lee JE, Kim BB, Ko Y, Park JB. Evaluation of the effects of Cimicifugae Rhizoma on the morphology and viability of mesenchymal stem cells. Exp Ther Med. 2015;10:629-634.
6
6. Zhang N, Kang T, Xia Y, Wen Q, Zhang X, Li H, et al. Effects of salvianolic acid B on survival, self-renewal and neuronal differentiation of bone marrow derived neural stem cells. Eur J Pharmacol. 2012;697:32-39.
7
7. Yang L, Wang NL, Cai GP. Maohuoside A promotes osteogenesis of rat mesenchymal stem cells via BMP and MAPK signaling pathways. Mol Cell Biochem. 2011;358:37-44.
8
8. Qian K, Xu H, Dai T, Shi K. Effects of Tanshinone IIA on osteogenic differentiation of mouse bone marrow mesenchymal stem cells. Naunyn Schmiedebergs Arch Pharmacol. 2015;388:1201-1209.
9
9. Cai M, Li G, Tao K, Yang Y, Lou L, Cai Z, et al. Maohuoside A acts in a BMP-dependent manner during osteogenesis. Phytother Res. 2013;27:1179-1184.
10
10. Kim DR, Lee JE, Shim KJ, Cho JH, Lee HC, Park SK, et al. Effects of herbal Epimedium on the improvement of bone metabolic disorder through the induction of osteogenic differentiation from bone marrow-derived mesenchymal stem cells. Mol Med Rep. 2017;15: 125-130.
11
11. Kim SY, An SY, Lee JS, Heo JS. Zanthoxylum schinifolium enhances the osteogenic potential of periodontal ligament stem cells. In Vitro Cell Dev Biol Anim. 2015;51:165-173.
12
12. Li J, Jiang Z, Li X, Hou Y, Liu F, Li N, et al. Natural therapeutic agents for neurodegenerative diseases from a traditional herbal medicine Pongamia pinnata (L.) Pierre. Bioorg Med Chem Lett. 2015;25:53-58.
13
13. Al Muqarrabun LM, Ahmat N, Ruzaina SA, Ismail NH, Sahidin I. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: a review. J Ethnopharmacol. 2013;150:395-420.
14
14. Brijesh S, Daswani PG, Tetali P, Rojatkar SR, Antia NH, Birdi TJ. Studies on Pongamia pinnata (L.) Pierre leaves: understanding the mechanism(s) of action in infectious diarrhea. J Zhejiang Univ Sci B. 2006;7:665-674.
15
15. Carcache-Blanco EJ, Kang YH, Park EJ, Su BN, Kardono LB, Riswan S, et al. Constituents of the stem bark of Pongamia pinnata with the potential to induce quinone reductase. J Nat Prod. 2003;66:1197-1202.
16
16. Jin SH, Lee JE, Yun JH, Kim I, Ko Y, Park JB. Isolation and characterization of human mesenchymal stem cells from gingival connective tissue. J Periodontal Res. 2015;50:461-467.
17
17. Ha DH, Yong CS, Kim JO, Jeong JH, Park JB. Effects of tacrolimus on morphology, proliferation and differentiation of mesenchymal stem cells derived from gingiva tissue. Mol Med Rep. 2016;14:69-76.
18
18. Liu YR, Qu SX, Maitz MF, Tan R, Weng J. The effect of the major components of Salvia Miltiorrhiza Bunge on bone marrow cells. J Ethnopharmacol. 2007;111:573-583.
19
19. Park JB, Zhang H, Lin CY, Chung CP, Byun Y, Park YS, et al. Simvastatin maintains osteoblastic viability while promoting differentiation by partially regulating the expressions of estrogen receptors alpha. J Surg Res. 2012;174:278-283.
20
20. Yazdani-Nasab Z, Piravi-Vanak Z. A Study on the Specifications of Cold Pressed Colza Oil. Recent Pat Food Nutr Agric. 2015;7:47-52.
21
21. Jeong SH, Jiang Y, Guo H, Wang MH, Park JB. Anti-inflammatory and anticancer effects of methanol, ethanol and water extracts of Asiasarum heterotropoide. Biomed Rex. 2016;27:103-109.
22
22. Mukudai Y, Zhang M, Shiogama S, Kondo S, Ito C, Motohashi H, et al. Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma. Evid Based Complement Alternat Med. 2016;2016:6087213.
23
23. Wang MH, Jeong SH, Guo H, Park JB. Anti-inflammatory and cytotoxic effects of methanol, ethanol, and water extracts of Angelicae Dahuricae Radix. J Oral Sci. 2016;58:125-131.
24
24. Wang F, Yu M, Yan X, Wen Y, Zeng Q, Yue W, et al. Gingiva-derived mesenchymal stem cell-mediated therapeutic approach for bone tissue regeneration. Stem Cells Dev. 2011;20:2093-2102.
25
25. Tomar GB, Srivastava RK, Gupta N, Barhanpurkar AP, Pote ST, Jhaveri HM, et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem Biophys Res Commun. 2010;393:377-383.
26
26. Li Z, Jiang CM, An S, Cheng Q, Huang YF, Wang YT, et al. Immunomodulatory properties of dental tissue-derived mesenchymal stem cells. Oral Dis. 2014;20:25-34.
27
27. Zhang QZ, Nguyen AL, Yu WH, Le AD. Human oral mucosa and gingiva: a unique reservoir for mesenchymal stem cells. J Dent Res. 2012;91:1011-1018.
28
28. Chang NJ, Chen YW, Shieh DE, Fang HY, Shie MY. The effects of injectable calcium silicate-based composites with the Chinese herb on an osteogenic accelerator in vitro. Biomed Mat. 2015;10:055004.
29
29. Wang XL, Xie XH, Zhang G, Chen SH, Yao D, He K, et al. Exogenous phytoestrogenic molecule icaritin incorporated into a porous scaffold for enhancing bone defect repair. J Orthop Res. 2013;31:164-172.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva
- Selection of the optimal herbal composition of pomegranate concentrated powder from aqueous extracts of Eucommiae Cortex and Achyranthis Radix to treat osteoarthritis in rats
- The Effects of Sa-Am Acupuncture on the Radial Pulse in Healthy Human Subjects: A Comparative Study of Pericardium jung-gyuck and Triple Energizer jung-gyuck
- A study on the traceability and uncertainty of skin hydration measurement on the sole of the foot
- Melanin Synthesis Inhibitory Effect of Eriobotryae Folium Extracts & Eriobotryae Folium and Phreatic Water Mixture
- A Proposal of Both Use of Direct Moxibustion and Electro-Moxibustion for Cancer Treatment Moxibustion
- Effects of Korean Medicine Health Promotion program for Children -Focus on Education and Prevention Programs-
- The Relationship between Epicardial Fat Thickness and Dampness-Phlegm Pattern in the Patients with ischemic stroke
- Effectiveness of Combined Korean Medicine on Traumatic Spinal Cord Injury: A Case Report
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.