- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2017, v.38 no.4, pp.1-10
(Department of Periodontics, College of Medicine, The Catholic University)
(Ethnobotanical Database of Bangladesh)
(Ethnobotanical Database of Bangladesh)
Salah,
U.
, &
(2017). The effects of Pongamia pinnata on osteogenic differentiation and mineralization of human stem cells derived from the gingiva. Journal of Korean Medicine, 38(4), 1-10.
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Abstract
Objectives: The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.
- keywords
- cell differentiation, herbal medicine, Pongamia, stem cells
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