Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Selection of the optimal herbal composition of pomegranate concentrated powder from aqueous extracts of Eucommiae Cortex and Achyranthis Radix to treat osteoarthritis in rats

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2017, v.38 no.4, pp.11-40
(Research Institute, HL Science Co., Ltd.,)


(Research Insitute, HL Science Co., Ltd.)
(Research Institute, HL Science Co., Ltd)


  • Downloaded
  • Viewed

Abstract

Objectives: We investigated whether a mixture of the main component of pomegranate concentrated powder (PCP) with appropriate proportions of Eucommiae Cortex (EC) and Achyranthis Radix (AR) could act synergistically as an effective treatment for osteoarthritis (OA). Methods: In order to evaluate the effects of PCP, EC, and AR against OA, knee thicknesses, maximum extension angle of each knee, anti – inflammation effects, the transcript levels of chondrogenic genes mRNA expressions in femur and tibia articular cartilage (AC) with synovial membrane (SM) were analyzed. In addition, the histopathology and immunohistochemistry of the femur and tibia AC or SM were performed. Results: The surgically-induced OA signs in rats were significantly inhibited by 28 days of continuous treatment of PCP, EC and AR single formulas, and PCP with EC:AR mixed formulas. Especially, PCP with EC:AR 4:1, 2:1 and 1:1 mixed formula treatment constantly showed significantly more favorable inhibitory activities, as compared with those of single formula of PCP, EC and AR treated rats. Conclusion: PCP and EC:AR 4:1 mixed formula showed similar OA refinement effects through potent anti-inflammatory pathways as compared with those of diclofenac treatment, and showed additional chondrocyte proliferating effects on the both femur and tibia AC.

keywords
Mixed formulation, Selections of the fittest mixed formula, Dried pomegranate concentrate powders, Eucommiae Cortex, Achyranthis Radix, Surgically-induced osteoarthritic rats.


Reference

1

1. Dougados M, Nguyen M, Berdah L, Mazieres B, Vignon E, Lequesne M, et al. Evaluation of the structure-modifying effects of diacerein in hip osteoarthritis: ECHODIAH, a three-year, placebo-controlled trial. Evaluation of the Chondromodulating Effect of Diacerein in OA of the Hip. Arthritis and rheumatism 2001; 44(11):2539-47.

2

2. Goldring MB, Goldring SR. Osteoarthritis. Journal of cellular physiology. 2007;213(3):626-34.

3

3. Pavelká K, Gatterová J, Olejarová M, Machacek S, Giacovelli G, Rovati LC. Glucosamine sulfate use and delay of progression of knee osteoarthritis: a 3-year, randomized, placebo -controlled, double-blind study. Archives of internal medicine. 2002;162(18):2113-23.

4

4. Fiorito S, Magrini L, Adrey J, Mailhé D, Brouty-Boyé D. Inflammatory status and cartilage regenerative potential of synovial fibroblasts from patients with osteoarthritis and chondropathy. Rheumatology (Oxford, England). 2005;44(2):164-71.

5

5. Qin J, Liu YS, Liu J, Li J, Tan Y, Li XJ, et al. Effect of Angelica sinensis Polysaccharides on Osteoarthritis In Vivo and In Vitro: A Possible Mechanism to Promote Proteoglycans Synthesis. Evid. Based Complement. Alternat. Med. 2013;2013:794761.

6

6. Na JY, Song KB, Kim SH, Kwon YB, Kim DG, Lee JK, et al. Effects of HPL-04 on degenerative osteoarthritis. J. Korean Soc. Food Sci. Nutr. 2014;43(1):30-9.

7

7. Felson DT, Zhang Y. An update on the epidemiology of knee and hip osteoarthritis with a view to prevention. Arthritis Rheum. 1998;41(8):1343-55.

8

8. Goldring MB, Fukuo K, Birkhead JR, Dudek E, Sandell LJ. Transcriptional suppression by interleukin-1 and interferon-gamma of type II collagen gene expression in human chondrocytes. J. Cell Biochem. 1994;54(1):85-99.

9

9. van de Loo FA, Joosten LA, van Lent PL, Arntz OJ, van den Berg WB. Role of interleukin-1, tumor necrosis factor a, and interleukin-6 in cartilage proteoglycan metabolism and destruction. Effect of in situ blocking in murine antigenand zymosan-induced arthritis. Arthritis Rheum. 1995;38(2):164-72.

10

10. Berenbaum F, Jacques C, Thomas G, Corvol MT, Bereziat G, Masliah J. Synergistic effect of interleukin-1b and tumor necrosis factor alpha on PGE2 production by articular chondrocytes does not involve PLA2 stimulation. Exp. Cell Res. 1996;222(2):379-84.

11

11. Grabowski PS, Macpherson H, Ralston SH. Nitric oxide production in cells derived from the human joint. Br. J. Rheumatol. 1996;35(3):207-12.

12

12. Stefanovic-Racic M, Morales TI, Taskiran D, McIntyre LA, Evans CH. The role of nitric oxide in proteoglycan turnover by bovine articular cartilage organ cultures. J. Immunol. 1996;156(3):1213-20.

13

13. Koolpe M, Pearson D, Benton HP. Expression of both P1 and P2 purine receptor genes by human articular chondrocytes and profile of ligand-mediated prostaglandin E2 release. Arthritis Rheum. 1999;42(2):258-67.

14

14. Felson DT, Neogi T. Osteoarthritis: is it a disease of cartilage or of bone? Arthritis and rheumatism. 2004;50(2):341-4.

15

15. Tamura T, Ohmori K. Rhein, an active metabolite of diacerein, suppresses the interleukin-1a -induced proteoglycan degradation in cultured rabbit articular chondrocytes. Jpn. J. Pharmacol. 2001;85(1):101-4.

16

16. Nam DE, Kim OK, Shim TJ, Kim JH, Lee JM. Effect of Boswellia serrata extracts on degenerative osteoarthritis in vitro and in vivo models. J. Korean Soc. Food Sci. Nutr. 2014;43(5):631-40.

17

17. Langley P. Why a pomegranate? BMJ (Clinical research ed.). 2000;321(7269):1153-4.

18

18. Gil MI, Tomás-Barberán FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J. Agric. Food Chem. 2000;48(10):4581-9.

19

19. Noda Y, Kaneyuki T, Mori A, Packer L. Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin. J. Agric. Food Chem. 2002;50(1):166-71.

20

20. Singh RP, Chidambara Murthy KN, Jayaprakasha GK. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. J. Agric. Food Chem. 2002;50(1):81-6.

21

21. Ahmed S, Wang N, Hafeez BB, Cheruvu VK, Haqqi TM. Punica granatum L. extract inhibits IL-1b-induced expression of matrix metalloproteinases by inhibiting the activation of MAP kinases and NF-kB in human chondrocytes in vitro. J. Nutr. 2005;135(9):2096-102.

22

22. Shukla M, Gupta K, Rasheed Z, Khan KA, Haqqi TM. Bioavailable constituents/metabolites of pomegranate (Punica granatum L) preferentially inhibit COX2 activity ex vivo and IL-1beta -induced PGE2 production in human chondrocytes in vitro. J. Inflamm. 2008;5:9.

23

23. Hadipour-Jahromy M, Mozaffari-Kermani R. Chondroprotective effects of pomegranate juice on monoiodoacetate-induced osteoarthritis of the knee joint of mice. Phytother. Res. 2010;24(2):182-5.

24

24. Rasheed Z, Akhtar N, Haqqi TM. Pomegranate extract inhibits the interleukin-1b-induced activation of MKK-3, p38a-MAPK and transcription factor RUNX-2 in human osteoarthritis chondrocytes. Arthritis Res. Ther. 2010;12(5):R195.

25

25. Hsieh CL, Yen GC. Antioxidant actions of du-zhong (Eucommia ulmoides Oliv.) toward oxidative damage in biomolecules. Life Sci. 2000;66(15):1387-400.

26

26. Kwan CY, Chen CX, Deyama T, Nishibe S. Endothelium-dependent vasorelaxant effects of the aqueous extracts of the Eucommia ulmoides Oliv. leaf and bark: implications on their antihypertensive action. Vascul. Pharmacol. 2003;40(5):229-35.

27

27. Zhao Y, Li Y, Wang X, Sun W. The experimental study of Cortex Eucommiae on meridian tropsim: the distribution study of aucubin in rat tissues. J. Pharm. Biomed. Anal. 2008;46(2):368-73.

28

28. Eum HA, Lee WY, Kim SH, Kim JY, Park SW, Lee JS, et al. Anti-inflammatory activity of CML-1: an herbal formulation. Am. J. Chin. Med. 2005;33(1):29-40.

29

29. Lee SY, Kwon HK, Lee SM. SHINBARO, a new herbal medicine with multifunctional mechanism for joint disease: first therapeutic application for the treatment of osteoarthritis. Arch. Pharm. Res. 2011;34(11):1773-7.

30

30. Li J, Li HJ, Li P, Qi H, Simultaneous qualitation and quantification of four phytoecdysones in Radix Achyranthis Bidentatae by high-performance liquid chromatography with diode array detection. Biomed. Chromatogr. 2007;21(8): 823-8.

31

31. Yu F, Li X, Cai L, Li H, Chen J, Wong X, et al. Achyranthes bidentata polysaccharides induce chondrocyte proliferation via the promotion of the G1/S cell cycle transition. Mol. Med. Rep. 2013;7(3):935-40.

32

32. Weng X, Lin P, Liu F, Chen J, Li H, Huang L, et al. Achyranthes bidentata polysaccharides activate the Wnt/b-catenin signaling pathway to promote chondrocyte proliferation. Int. J. Mol. Med. 2014;34(4):1045-50.

33

33. Kim JW, Cho HR, Ku S., Efficacy test of Polycan, a beta-glucan originated from Aureobasidium pullulans SM-2001, on anterior cruciate ligament transection and partial medial meniscectomy-induced-osteoarthritis rats. Journal of microbiology and biotechnology. 2012;22(2):274-82.

34

34. Kang SJ, Kim JW, Kim KY, Ku SK, Lee YJ. Protective effects of calcium gluconate on osteoarthritis induced by anterior cruciate ligament transection and partial medial meniscectomy in Sprague-Dawley rats. Journal of orthopaedic surgery and research. 2014;9(1):14.

35

35. Moon CH, Kwon O, Woo CH, Ahn HD, Kwon YS, Park SJ, et al. Therapeutic effect of irradiation of magnetic infrared laser on osteoarthritis rat model. Photochemistry and photobiology. 2014;90(5):1150-9.

36

36. Choi JS, Shin HS, Kim KY, Ku SK, Choi IS, Kim JW. Effect of Polycalcium, a mixture of Polycan and calcium lactate-gluconate in a 1:9weight ratio, on rats with surgery-induced osteoarthritis. Exp. Ther. Med. 2015;9(5):1780-90.

37

37. Rezende MU, Gurgel HM, Vilaça Junior PR, Kuroba RK, Lopes AS, Phillipi RZ, et al. Diacerhein versus glucosamine in a rat model of osteoarthritis. Clinics (Sao Paulo, Brazil). 2006;61(5):461-6.

38

38. Rai MF, Graeve T, Twardziok S, Schmidt MF. Evidence for regulated interleukin-4 expression in chondrocyte-scaffolds under in vitro inflammatory conditions. PloS one. 2011;6(10):e25749.

39

39. Innes JF, Gordon C, Vaughan-Thomas A, Rhodes NP, Clegg PD. Evaluation of cartilage, synovium and adipose tissue as cellular sources for osteochondral repair. Veterinary journal (London, England : 1997). 2013;197(3):619-24.

40

40. Hwang YI, Yoo YB, Baik SH. Comparative study of rat thyroid regeneration using PCNA and BrdU immunohistochemistry. Korean J Anat. 2000;33(2):247-54.

41

41. Barrett KL, Willingham JM, Garvin AJ, Willingham MC. Advances in cytochemical methods for detection of apoptosis. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2001;49(7):821-32.

42

42. Hwangbo M, Jung JY, Ki SH, Park SM, Jegal KH, Cho IJ, et al. U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-kB -Mediated Inducible Nitric Oxide Synthase. Evidence-based complementary and alternative medicine : eCAM. 2014;2014:542825.

43

43. Lee CW, Park SM, Kim YS, Jegal KH, Lee JR, Cho IJ, et al. Biomolecular evidence of anti-inflammatory effects by Clematis mandshurica Ruprecht root extract in rodent cells. Journal of ethnopharmacology. 2014;155(2):1141-55.

44

44. Sailer ER, Schweizer S, Boden SE, Ammon HP, Safayhi H. Characterization of an acetyl-11-keto-b-boswellic acid and arachidonate-binding regulatory site of 5-lipoxygenase using photoaffinity labeling. Eur. J. Biochem. 1998;256(2):364-8.

45

45. Guo JS, Ou L, Zhou J, Wang XJ, Guo X. Impact on the model of rat osteoarthritis of jingu tablet. Zhongguo Zhong yao za zhi =Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2006;31(3):232-5.

46

46. Hardy MM, Seibert K, Manning PT, Currie MG, Woerner BM, Edwards D, et al. Cyclooxygenase 2-dependent prostaglandin E2modulates cartilage proteoglycan degradation in human osteoarthritis explants. Arthritis Rheum. 2002;46(7):1789-803.

47

47. Bresnihan B. Pathogenesis of joint damage in rheumatoid arthritis. J. Rheumatol. 1999;26(3):717-9.

48

48. Nagase H, Woessner JF, Jr. Matrix metalloproteinases. J. Biol. Chem. 1999;274(31):21491-4.

49

49. Halliwell B. Oral inflammation and reactive species: a missed opportunity? Oral Dis. 2000;6(3):136-7.

50

50. Permuy M, Guede D, López-Peña M, Muñoz F, Caeiro JR, González-Cantalapiedra A. Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model. BMC veterinary research. 2015;11:143.

51

51. Permuy M, Guede D, López-Peña M, Muñoz F, Caeiro JR, González-Cantalapiedra A. Comparison of various SYSADOA for the osteoarthritis treatment: an experimental study in rabbits. BMC musculoskeletal disorders. 2015;16:120.

52

52. Armstrong S, Read R, Ghosh P. The effects of intraarticular hyaluronan on cartilage and subchondral bone changes in an ovine model of early osteoarthritis. The Journal of rheumatology. 1994;21(4):680-8.

53

53. Nuñez G, Benedict MA, Hu Y, Inohara N. Caspases: the proteases of the apoptotic pathway. Oncogene. 1998;17(25):3237-45.

54

54. Trucco C, Oliver FJ, de Murcia G, Menissier-de Murcia J. DNA repair defect in poly (ADP-ribose) polymerase-deficient cell lines. Nucleic acids research. 1998;26(11):2644-9.

55

55. Smulson ME, Pang D, Jung M, Dimtchev A, Chasovskikh S, Spoonde A, et al. Irreversible binding of poly(ADP)ribose polymerase cleavage product to DNA ends revealed by atomic force microscopy: possible role in apoptosis. Cancer research. 1998;58(16):3495-8.

56

56. Cole KK, Perez-Polo JR. Poly(ADP-ribose)polymerase inhibition prevents both apoptotic -like delayed neuronal death and necrosis after H2O2 injury. Journal of neurochemistry. 2002;82(1):19-29.

57

57. Cheng W, Wu D, Zuo Q, Wang Z, Fan W. Ginsenoside Rb1 prevents interleukin-1 beta induced inflammation and apoptosis in human articular chondrocytes. International orthopaedics. 2013;37(10):2065-70.

58

58. Xue H, Tu Y, Ma T, Liu X, Wen T, Cai M, et al. Lactoferrin Inhibits IL-1b-Induced Chondrocyte Apoptosis Through AKT1-Induced CREB1Activation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 2015;36(6):2456-65.

59

59. Ganey T, Libera J, Moos V, Alasevic O, Fritsch KG, Meisel HJ, et al. Disc chondrocyte transplantation in a canine model: a treatment for degenerated or damaged intervertebral disc. Spine. 2003;28(23):2609-20.

60

60. Moore EE, Bendele AM, Thompson DL, Littau A, Waggie KS, Reardon B, et al. Fibroblast growth factor-18 stimulates chondrogenesis and cartilage repair in a rat model of injury-induced osteoarthritis. Osteoarthritis and cartilage. 2005;13(7):623-31.

61

61. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol. Pathol. 2003;31(6):619-24.

62

62. Mundlos S, Olsen BR. Heritable diseases of the skeleton. Part I: Molecular insights into skeletal development-transcription factors and signaling pathways. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 1997;11(2):125-32.

63

63. Warnock JJ, Spina J, Bobe G, Duesterdieck -Zellmer KF, Ott J, Baltzer WI, et al. Culture of canine synoviocytes on porcine intestinal submucosa scaffolds as a strategy for meniscal tissue engineering for treatment of meniscal injury in dogs. Veterinary journal (London, England : 1997). 2014;199(1):49-56.

상단으로 이동

Journal of Korean Medicine