- P-ISSN 1010-0695
- E-ISSN 2288-3339
- Apply for Authority
- P-ISSN1010-0695
- E-ISSN2288-3339
Article Detail
Home > Article Detail
Effects of Curcuma longa Rhizoma on MIA-induced Osteoarthritis in Rat Model
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2019, v.40 no.3, pp.35-58
https://doi.org/10.13048/jkm.19026
(2019). Effects of Curcuma longa Rhizoma on MIA-induced Osteoarthritis in Rat Model. Journal of Korean Medicine, 40(3), 35-58, https://doi.org/10.13048/jkm.19026
- Downloaded
- Viewed
Abstract
Objectives: The aim of this study was to investigate the anti-inflammatory effects of Curcuma longa rhizoma extract in an experimental rat model of osteoarthritis. Methods: Osteoarthritis was induced in rats by injecting monosodium iodoacetate (MIA) into the knee joint cavity of rats. The rats were divided into 5 groups (Normal, Control, positive comparison, low (CL) and high (CH) concentration groups). Rats in the low concentration (CL) group had MIA-induced osteoarthritis; they were treated with Curcuma longa rhizoma extract at a dose of 50㎎/㎏ body weight. Rats in the high concentration (CH) group had MIA-induced osteoarthritis; they were treated with Curcuma longa rhizoma extract at a dose of 100㎎/㎏ body weight. Hind paw weight distribution and ROS levels were measured. At the end of all treatments, changes in alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine levels were analyzed. In addition, inflammatory protein levels were evaluated by western blot analysis. Results: In this study, hind paw weight distribution significantly improved in the CL and CH groups, while. Reactive oxygen species (ROS) production significantly decreased in both. The levels of ALT, AST, BUN, and creatinine did not significantly change in either group. The production of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4), p47phox, and Ras-related C3 botulinum toxin substrate 1 (RAC1) decreased in both. Catalase, heme oxygenase-1 (HO-1) and superoxide dismutase (SOD) significantly increased in the CL and CH groups, respectively. Nuclear factor erythroid 2 (Nrf2) increased, but there were no significant differences between the experimental and control groups. Inflammatory cytokines, including nuclear factor-kappa Bp65 (NF-κBp65), interleukin-1beta (IL-1β), and tumor necrosis factor-alpha (TNF-α), decreased significantly in both the CL and CH groups. Conclusions: Our results showed that Curcuma longa rhizoma extract has anti-inflammatory effects. Anti-inflammatory activity is regulated by the inhibition of inflammatory cytokines and mediators, such as NF-κB, therefore, it suppresses cartilage damage as well.
- keywords
- Curcuma longa rhizoma extract, Osteoarthritis, Anti-inflammation, Monosodium iodoacetate (MIA)
Reference
1
1. The Society of Korean Medicine Rehabilitation. Korean Rehabilitation Medicine 4th ed. Seoul:Koonja publisher. 2015:102-51.
2
2. Korean Acupuncture & Moxibustion Medicine Society. Acupuncture Medicine. Seoul:Hanmibook. 2016:537-68.
3
3. Harrison's Principles of Internal Medicine Edit committee of the Korean Association of Internal Medicine. Harrison's Principles of Internal Medicine 18th edition(2). Seoul:MIP. 2013:2782.
4
4. Herbal medicine editorial committee of Korean medicine college. Herbal medicine. Seoul:Younglimsa. 2011:454-5.
5
5. Jung TS, Choi CW. The Effect of the Curcumae Longae Rhizoma (CLR) Extract on the Hepatocellular Carcinogenesis and Acute Liver Damage Induced by Diethylnitrosamine (DENA)and CCl4 in Rats. Herbal Formula Science. 2014;22(1):177-92.
6
6. Oh JS, Yang SY, Kim MH, Namgung O, Park YC. Effects of Root of Curcumin longa on LPS-induced Lung Injury. J Korean Oriental Med. 2013;34(1):89-102.
7
7. Kim SD. Effects of Curcuma longa Rhizoma (CLR) on chronic renal failure in rats. Dongshin University Graduate School. 2008.
8
8. Lee JH, Kim JH, Park SY, Choi JH. Effects of Curcuma longa Rhizoma on Asthma induced intra-nasal instillation of Ovalbumin in Mice. The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology. 2008;21(3):20-35.
9
9. Seong KW. The Effect of Oral Curcuma longa ingestion on the gene expression in a Rat Model of Benign Prostatic Hyperplasia using microarray analysis. Kyunghee University Graduate School. 2012.
10
10. Lee JH. Effects of Curcumae Longae Rhizoma pharmacopuncture on MIA-induced Osteoarthritis Rats. Daegu Haany University Graduate School. 2018.
11
11. Lee OJ, Lee DG, Lee JH, Lee JH, Lee SH, Park GH, et al. Effects of Curcuma longa LINNE Pharmacopuncture at ST36 on Adjuvant Induced Arthritis in Rats. The Acupuncture. 2013;30(4):95-105.
12
12. Yang H, Huang S, Wei Y, Cao S, Pi C, Feng T, et al. Curcumin Enhances the Anticancer Effect Of 5-fluorouracil against Gastric Cancer through Down-Regulation of COX-2 and NF-κB Signaling Pathways. J Cancer. 2017;8(18):3697-706.
13
13. Haryuna TS, Munir D, Maria A, Bashiruddin J. The Antioxidant Effect of Curcumin on Cochlear Fibroblasts in Rat Models of Diabetes Mellitus. Iran J Otorhinolaryngol. 2017;29(93):197-202.
14
14. Wang X, Hang Y, Liu J, Hou Y, Wang N, Wang M. Anticancer effect of curcumin inhibits cell growth through miR-21/PTEN/Akt pathway in breast cancer cell. Oncol Lett. 2017;13(6):4825-31.
15
15. Mohammadi S, Karimzadeh Bardei L, Hojati V, Ghorbani AG, Nabiuni M. Anti-Inflammatory Effects of Curcumin on Insulin Resistance Index, Levels of Interleukin-6, C-Reactive Protein, and Liver Histology in Polycystic Ovary Syndrome-Induced Rats. Cell J. 2017;19(3):425-33.
16
16. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Mono-iodoacetate-induced Histologic Changes in Subchondral Bone and Articular Cartilage of Rat Femorotibial Joints: an Animal Model of Osteoarthritis. Toxicol Pathol. 2003;31(6):619-24.
17
17. Ratanavaraporn J, Soontornvipart K, Shuangshoti S, Shuangshoti S, Damrongsakkul S. Localized Delivery of Curcumin from Injectable Gelatin/Thai Silk Fibroin Microspheres for Anti-inflammatory Treatment of Osteoarthritis in a Rat Model. Inflammopharmacology. 2017;25(2):211-21.
18
18. Park S, Lee LR, Seo JH, Kang S. Curcumin and Tetrahydrocurcumin Both Prevent Osteoarthritis Symptoms and Decrease the Expressions of Pro-inflammatory Cytokines in Estrogen -deficient Rats. Genes Nutr. 2016;11:2.
19
19. Niazvand F, Khorsandi L, Abbaspour M, Orazizadeh M, Varaa N, Maghzi M, et al. Curcumin-loaded Poly Lactic-co-glycolic Acid Nanoparticles Effects on Mono-iodoacetate -induced Osteoarthritis in Rats. Vet Res Forum. 2017;8(2):155-61.
20
20. Li XS, Chen H, Zhen P, Li SS, Zhou SH, Tian Q, et al. JAK2/STAT3 Signal Pathway Mediating Curcumin in Cartilage Cell Metabolism of Osteoarthritis. Zhongguo Gu Shang. 2016;29(12):1104-9.
21
21. Wang J, Ma J, Gu JH, Wang FY, Shang XS, Tao HR, et al. Regulation of Type II Collagen, Matrix Metalloproteinase-13 and Cell Proliferation by Interleukin-1β Is Mediated by Curcumin via Inhibition of NF-κB Signaling in Rat Chondrocytes. Mol Med Rep. 2017;16(2):1837-45.
22
22. Li X, Feng K, Li J, Yu D, Fan Q, Tang T, et al. Curcumin Inhibits Apoptosis of Chondrocytes through Activation ERK1/2 Signaling Pathways Induced Autophagy. Nutrients. 2017;9(4):414.
23
23. Henrotin Y, Gharbi M, Dierckxsens Y, Priem F, Marty M, Seidel L, et al. Decrease of a Specific Biomarker of Collagen Degradation in Osteoarthritis, Coll2-1, by Treatment with Highly Bioavailable Curcumin during an Exploratory Clinical Trial. BMC Complement Altern Med. 2014;14:159.
24
24. Nakagawa Y, Mukai S, Yamada S, Matsuoka M, Tarumi E, Hashimoto T, et al. Short-term Effects of Highly-bioavailable Curcumin for Treating Knee Osteoarthritis: a Randomized, Double-blind, Placebo-controlled Prospective study. J Orthop Sci. 2014;19(6):933-9.
25
25. Haroyan A, Mukuchyan V, Mkrtchyan N, Minasyan N, Gasparyan S, Sargsyan A, et al. Efficacy and Safety of Curcumin and Its Combination with Boswellic Acid in Osteoarthritis: a Comparative, Randomized, Double-blind, Placebo-controlled Study. BMC Complement Altern Med. 2018;18(1):7.
26
26. Ramadan G, Al-Kahtani MA, El-Sayed WM. Anti-inflammatory and Anti-oxidant Properties of Curcuma longa (turmeric) versus Zingiber officinale (Ginger) Rhizomes in Rat Adjuvant -induced Arthritis. Inflammation. 2011;34(4):291-301.
27
27. Funk JL, Oyarzo JN, Frye JB, Chen G, Lantz RC, Jolad SD, et al. Turmeric Extracts Containing Curcuminoids Prevent Experimental Rheumatoid Arthritis. J Nat Prod. 2006;69(3):351-5.
28
28. Blois MS. Antioxidant Determinations by the Use of a Stable Free Radical. Nature. 1958;181(4617):1199–200.
29
29. Seo CS, Kim JH, Shin HK, Kim BS. Quantitative Analysis of (+)-Catechin, Paeoniflorin, and Paeonol in Moutan Radicis Cortex and Its Processed Products. Kor. J. Pharmacogn. 2016;47(3):237-45.
30
30. Kooy NW, Royall JA, Ischiropoulos H, Beckman JS. Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med. 1994;16(2):149-56.
31
31. Guccione AA, Felson DT, Anderson JJ, Anthony JM, Zhang Y, Wilson PW, et al. The Effects of Specific Medical Conditions on the Functional Limitations of Elders in the Framingham Study. Am J Public Health. 1994;84(3):351–8.
32
32. Felson DT, Zhang Y. An Update on the Epidemiology of Knee and Hip Osteoarthritis with a View to Prevention. Arthritis Rheum. 1998;41(8):1343-55.
33
33. Ministry of the Interior and Safety. Population census. [cited 2018 Mar 05]. Available from:URL: http://www.mois.go.kr/frt/bbs/type010/commonSelectBoardArticle.do?bbsId=BBSMST R_000000000008&nttId=59453.
34
34. Jeon MH, Kim MH. Effect of Hijikia fusiforme Fractions on Proliferation and Differentiation in Osteoblastic MC3T3-E1 Cells. Journal of Life Science. 2011;21(2):300-8.
35
35. Kang YG, Nam JH, Lee KS. The Effect of Indomethacin on the Matrix Metalloproteinases in Canine Permanent Tooth Eruption. Korean J Orthod. 2006;36(2):91-102.
36
36. Kim HA. Pharmacological Treatment of Osteoarthritis. J. of Korean Orthopaedic Research Society. 2010;13(1):16-22.
37
37. Korean Knee Society Subcommittee on Osteoarthritis Guidelines. Guidelines for the Treatment of Osteoarthritis of the Knee. J Korean Knee Soc. 2010;22(1):69-74.
38
38. Park SW, Kim YS, Lee DH, Kwon YB, Park JY, Lee SY, et al. Efficacy and Safety of HT008 and Glucosamine Sulfate in the Treatment of Knee Osteoarthritis : A Randomized Double-blind Trial. Kor. J. Herbology. 2014;29(4):45-52.
39
39. Ahn SI, Bok JH, Son JY. Antioxidative Activity and Nitrite-scavenging Abilities of Some Phenolic Compounds. Korean J. Food Cookery Sci. 2007;23(1):19-24.
40
40. Lee JA, Jung DS. Screening of the Antioxidant and Anti-elastase Activities for the Extracts of Jeju Endemic Plants. Journal of Research in Education & Science. 2011;13(2):249-69.
41
41. Kim SJ, Kim HJ, Park JC. DPPH Radical Scavenging Effects of the Aerial Parts of Fagopyrum esculentum and Isolation of Bioactive Flavonoids. Herbal formula science. 2004;12(1):255-62.
42
42. Lee YM, Bae JH, Jung HY, Kim JH, Park DS. Antioxidant Activity in Water and Methanol Extracts from Korean Edible Wild Plants. J Korean Soc Food Sci Nutr. 2011;40(1):29-36.
43
43. Beckman KB, Ames BN. The free radical theory of aging matures. Physiol Rev. 1998;78(2):547-81.
44
44. McCord JM. The Evolution of Free Radicals and Oxidative Stress. Am J Med. 2000;108(8):652-9.
45
45. Bedard K, Krause KH. The NOX family of ROS-generating NADPH Oxidases: Physiology and Pathophysiology. Physiol Rev. 2007;87(1):245-313.
46
46. Morel F, Doussiere J, Vignais PV. The Superoxide -generating Oxidase of Phagocytic Cells. Physiological, Molecular and Pathological Aspects. Eur J Biochem. 1991;201(3):523-46.
47
47. Vignais PV. The Superoxide-generating NADPH Oxidase: Structural Aspects and Activation Mechanism. Cell Mol Life Sci. 2002;59(9):1428-59.
48
48. Radermacher KA, Wingler K, Kleikers P, Altenhöfer S, Hermans JJR, Kleinschnitz C, et al. The 1027th Target Candidate in Stroke:Will NADPH Oxidase Hold Up?. Exp Transl Stroke Med. 2012;4:1-11.
49
49. Jin BM, Lee MK, Lee JS, Hyun KY. Anti-inflammatory Effects of Korean red ginseng Extract in formalin-induced Orofacial Pain in Rats. Journal of the Korea Academia -Industrial cooperation Society. 2014;15(9):5708-15.
50
50. Lim JS, Ahn KY. Potassium Depletion Upregulates Expression of Nrf2 Transcription Factor in Rat Kidney. Korean J Nephrol. 2011;30(3):239-45.
51
51. Lemos FB, Ijzermans JN, Zondervan PE, Peeters AM, Mol WM, Weimar W, et al. Differential Expression of Heme Oxygenase-1and Vascular Endothelial Growth Factor in Cadaveric and Living Donor Kidneys after Ischemia-Reperfusion. J Am Soc Nephrol. 2003;14:3278–87.
52
52. Van Raamsdonk JM, Hekimi S. Superoxide Dismutase Is Dispensable for Normal Animal Lifespan. Proc Natl Acad Sci USA. 2012;109(15):5785-90.
53
53. Preiser JC. Oxidative Stress. JPEN J Parenter Enteral Nutr. 2012;36(2):147-54.
54
54. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Selenium:Biochemical Role as a Component of Glutathione Peroxidase. Science. 1973;179(4073):588-90.
55
55. Park SH, Lee HJ, Ryu JH, Lee SY, Shin HD, Hong JH, et al. Effects of Silibinin and Resveratrol on Degradation of IκB and Translocation of NF-κB p65 Induced by Tumor Necrosis Factor-α in Cultured Airway Epithelial Cells. Yakhak Hoeji. 2014;58(1):1-6.
56
56. Asagiri M, Takayanagi H. The Molecular Understanding of Osteoclast Differentiation. Bone. 2007;40(2):251-64.
57
57. Lee SM, Kim YK, Hwang YH, Lee CH, Lee HS, Lee CT, et al. Role of PI3K/Akt Pathway in the Activation of IκB/NF-κB Pathway in Lung Epithelial Cells. Tuberculosis and Respiratory Diseases. 2003;54(5):551-62.
58
58. Abramson SB. Osteoarthritis and Nitric Oxide. Osteoarthritis Cartilage. 2008;16:15-20.
59
59. Sakaguchi Y, Shirahase H, Ichikawa A, Kanda M, Nozaki Y, Uehara Y. Effects of Selective iNOS Inhibition on Type II Collagen-induced Arthritis in Mice. 2004;75(19):2257-67.
60
60. Iwanami K, Matsumoto I, Tanaka-Watanabe Y, Inoue A, Mihara M, Ohsugi Y, et al. Crucial Role of the Interleukin-6/interleukin-17 Cytokine Axis in the Induction of Arthritis by Glucose -6-phosphate Isomerase. Arthritis Rheum. 2008;58(3):754-63.
61
61. Hulejová H, Baresová V, Klézl Z, Polanská M, Adam M, Senolt L. Increased Level of Cytokines and Matrix Metalloproteinases in Osteoarthritic Subchondral Bone. Cytokine. 2007;38(3):151-6.
62
62. Halliwell B. Oral inflammation and reactive species: a missed opportunity?. Oral Dis. 2000;6(3):136-7.
63
63. Zeng ZS, Cohen AM, Guillem JG. Loss of Basement Membrane Type IV Collagen Is Associated with Increased Expression of Metalloproteinases 2 and 9 (MMP-2 and MMP-9) during Human Colorectal Tumorigenesis. Carcinogenesis. 1999;20(5):749-55.
64
64. Nagase H, Woessner JF. Matrix Metalloproteinases. J Biol Chem. 1999;274(31):21491-4.
65
65. Hayakawa T. Tissue Inhibitors of Metalloproteinases and their Cell Growth-promoting Activity. Cell Struct Funct. 1994;19(3):109-14.
66
66. Kwon OJ, Kim MY, Shin SH, Lee AR, Lee JY, Seo BI, et al. Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats. Evid Based Complement Alternat Med. 2016:1-13.
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Association between Korean Medicine Hospital Utilization and Cardiovascular Risks in Patients with Hypertension: a National Korean Cohort Study
- A Qualitative Study on Steering of Education Accreditation: With Case Study on Korean Medicine Education
- Effects of Curcuma longa Rhizoma on MIA-induced Osteoarthritis in Rat Model
- A study on the current status and development of the new health technology assessment of Korean medicine field
- A Study Assessing 2018 Gyenggi-do Korean medicine Support Project for Subfertility
- Analysis of Korean Medical status of Acute Bronchitis, Chronic Bronchitis and Allergic Rhinitis patients
- The Effect of Moxibustion Treatment for Cardiac Arrhythmia : A Systematic Review
- A Review on the Characteristics of Temperature Variation in Warm Needle
- A Review on Safety of Herbal Medicines for Doping
- A Rehabilitation protocol for Arthroscopic Bankart repair in Korean Medicine : A Case Report
- The Statistics Study on Patients visiting a Korean Medicine Hospital according to the Application of Chuna Therapy Health Insurance and Medical benefits
- The Effect of Korean Medical Treatments for Facial asymmetry Patients : Five Cases Report
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.