- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Antioxidant Activity and Anti-inflammatory Effect of Extracts from Paulownia tomentosa in LPS-stimulated RAW264.7 macrophage cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2019, v.40 no.4, pp.72-83
https://doi.org/10.13048/jkm.19042
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(2019). Antioxidant Activity and Anti-inflammatory Effect of Extracts from Paulownia tomentosa in LPS-stimulated RAW264.7 macrophage cells. Journal of Korean Medicine, 40(4), 72-83, https://doi.org/10.13048/jkm.19042
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Abstract
In this study, we investigated the antioxidant and anti-inflammatory effect of the Paulownia tomentosa extracts (PTE). The total polyphenol and flavonoid contents of PTE were 148.98±1.84 mg GAE/g extract, and 115.33±4.16 mg CE/g extract, respectively. The PTE showed that strong antioxidant activity via -diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ABTS radical scavenging activity and FRAP assay. The anti-inflammatory activity was evaluated on lipopolysaccharide (LPS)-stimulated RAW264.7 cells. PTE remarkably reduced protein expression of inducible nitric oxide (iNOS), resulting in inhibition of production of nitric oxide (NO). Additionally, pre-treatment of PTE significantly suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Moreover, PTE significantly attenuated LPS-induced IkappaB (IκB) degradation and suppressed nuclear factor kappa B (NF-κB) nuclear translocation in macrophages. The PTE showed high antioxidant and anti-inflammatory activity. These data suggest that PTE has pharmacological activity and may be useful for the development of anti-inflammatory agents.
- keywords
- Paulownia tomentosa, Anti-inflammatory effect, Nitric oxide (NO), RAW264.7 cells
Reference
1
1. Kryston TB, Georgiev AB, Pissis P. Georgakilas AG. Role of oxidative stress and DNA damage in human carcinogenesis. Mutation Research -Fundamental and Molecular Mechanisms of Mutagenesis 2011;711(1):193-201.
2
2. White PAS, Oliveira RCM, Oliveira AP, Serafini MR, Araujo AAS, Gelain DP et al. Antioxidant Activity and Mechanism of Action of Natural Compounds Isolated from Lichens: A Systematic Review. Molecules 2014;19:14496-527.
3
3. Brewer, MS. Natural Antioxidants: Sources, Compounds, Mechanism of Action, and Potential Applications. Compr Rev Food Sci Food Saf 2011;10:221-47.
4
4. Eun CS, Hwang EY, Lee SO, Yang SA, Yu MH. Anti-oxidant and Anti-inflammatory Activities of Barley Sprout Extract. J Life Science 2016;26(5):537-44.
5
5. Kim KA, Yi HS, Yun HJ, Park SD. Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Cynomorium Songaricum. Korean J Orient Physiology &Pathology 2009;23(6):1320-31.
6
6. Yamauchi T, Date H, Saihara Y, Osada K. Deodorants Containing Hydrogen Peroxide and Plant Extracts. Jpn. Kokai Takkyo koho JP; 1987. p.62-357.
7
7. Kim MS, Lee DC, Hong JE, Chang IS, Cho HY, Kwon YK, Kim HY. Antimicrobial Effects of Ethanol Extracts from Korean and Indonesian Plants. Korean Journal of Food Science and Technology 2000;32(4):949-958.
8
8. Park YM, Jang SK, Kim YS, Kim BK. The constituents of Paulwnia stem. Yakhak Hoeji 1991;31:301-307.
9
9. Jun BS, Cha JY, Cho YS. Antioxidative Activities of Fruit Extracts of Paulownia tomentosa Stued. Korean J. Food Preserv 2001;8(2):231-238.
10
10. Singleton VL, Rossi JAJ. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Viticult. 16:144-158 (1965)
11
11. Jia Z, Tang M, Wu J. 1999. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555-559.
12
12. Lee SJ, Kim EK, Hwang JW, Oh HJ, Yang SI, Lee SJ, Jeon BT, Kim SK, Park PJ. 2009. Free radical scavenging activity of β-chitooligosaccharides. J Chitin Chitosan 14:24-28.
13
13. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237.
14
14. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal. Biochem. 239: 70-76 (1996)
15
15. Berridge MV, Tan AS (1993) Characterization of the cellular reduction of 3-(4,5-dimethylthiazol -2yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys, 303, 474-482.
16
16. Schnyder-Candrian, S., V. F. Quesniaux, F. Di Padova, I. Maillet, N. Noulin, I. Couillin, R. Moser, F. Erard, B. B. Vargaftig, B. Ryffel, and B. Schnyder (2005) Dual effects of p38 MAPK on TNFdependent bronchoc onstriction and TNF-independent neutrophil recruitment in lipopolysaccharide-induced acute respiratory distress syndrome. J. Immunol. 175:262-269.
17
17. Zhong, J. and J. M. Kyriakis (2007) Dissection of a signaling pathway by which pathogen -associated molecular patterns recruit the JNK and p38 MAPKs and trigger cytokine release. J. Biol. Chem. 282: 24246-24254
18
18. Hawiger, J. (2001) Innate immunity and inflammation: A transcriptional paradigm. Immunol. Res. 23: 99-109.
19
19. Nam, N. H. (2006) Naturally occurring NF-κB inhibitors. Mini Rev. Med. Chem. 6:945-951.
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