편평상피폐암에서 암억제유전자 RASSF1A의 메틸화와 임상 및 병리소견과의 연관성
Association between RASSF1A Methylation and Clinicopathological Factors in Patients with Squamous Cell Carcinoma of Lung
임대식 (한국과학기술원)
손대순 (삼성생명과학연구소)
김진국 (삼성서울병원)
김호중 (성균관대학교)
최용수 (성균관대학교)
이혜숙
송인승
임유성
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Abstract
Background:RASSF1A, which is one of tumor suppressor genes, is frequently inactivated by hypermethylation of the promoter region in a variety of human cancers, including lung cancer. This study was performed to investigate the association between RASSF1A methylation and the clinicopathological factors in patients with squamous cell carcinoma of the lung. Methods:Eighty-one samples from the patients with squamous cell carcinoma of lung were examined. The promoter methyation of RASSF1A was analyzed by methylation specific PCR and sequencing. Statistical analysis was made to examine the association between RASSF1A methylation and the clinicopathological parameters. Results:RASSF1A methylation was observed in 37.0 % (30 of 81) of the patients with squamous cell carcinoma of the lung. RASSF1A methylation was found to be associated with cellular differentiation(p=0.0097) and the overall survival(p=0.0635). However, there was no association between RASSF1A methylation and the other clinicopathological parameters, such as the pathological TNM stage, the recurrence rate, lymph node invasion and the amount of cigarettes smoked. Conclusion:RASSF1A methylation might be associated with a poor prognosis in patients with squamous carcinoma of the lung. A larger scale study is needed.(Tuberc Respir Dis 2004; 57:265-272)
- keywords
- Methylation, Squamous cell carcinoma, Tumor suppressor gene.편평상피폐암에서 암억제유전자 RASSF1A의 메틸화와 임상 및 병리소견과의 연관성성균관대학교 의과대학 삼성생명과학연구소 임상의학센터 암연구센터1, 호흡기내과2, 흉부외과3, 한국과학기술원 생명과학과4최내윤1, 이혜숙1, 송인승1, 임유성1, 손대순1, 임대식4, 최용수3, 김진국3, 김호중2Association between RASSF1A
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