결핵성 흉막염의 진단에서 결핵균 항원 자극에 의한 흉수 세포의 IFN-γ분비 검사의 유용성
The Utility of Pleural Fluid Cell IFN-γ Production Assay in the Diagnosis of Tuberculous Pleurisy
지영구 (단국대학교)
조성애 (연세대학교)
최주영 (연세대학교)
조상래 (연세대학교)
김윤섭 (단국대학교)
이계영 (단국대학교)
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초록
연구배경 : 결핵균 항원자극에 의한 흉수 세포의 IFN-γ 분비검사가 결핵성 흉막염과 다른 원인의 흉수의 감별진단에 유용한지 알아보고자 하였다. 방 법 : 39명의 결핵성 흉막염 환자와 비결핵성 흉수를 보인 26명의 환자(부폐렴성 흉수 13명, 암성 흉수 13명)들의 흉수세포를 분리하여 배양하면서 PPD와 PHA로 24시간 자극한 후 배양상층액의 IFN-γ 농도를 ELISA 방법으로 측정하였다.
- keywords
- Tuberculosis, Pleurisy, PPD, IFN-γ, Tuberculosis, Pleurisy, PPD, IFN-γ
Abstract
Background : Diagnosis of tuberculous pleurisy is sometimes difficult using conventional diagnostic methods. We have investigated the utility of pleural fluid cell IFN-γ production assay in the diagnosis of tuberculous pleurisy. Methods : We prospectively performed pleural fluid cell IFN-γ production assay in 39 patients with tuberculous pleural effusions (TPE) and in 26 patients with nontuberculous pleural effusions (NTPE) (13 malignant pleural effusions and 13 parapneumonic effusions). Pleural fluid cells were cultured in DMEM media and stimulated with purified protein derivatives (PPD), and phytohemagglutinin (PHA) for 24 hr. The amount of IFN-γ released in the culture supernatant was quantitated by IFN-γ ELISA assay. We have also measured adenosine deaminase (ADA) activities and IFN-γ concentrations in the pleural fluid. Results : 1) The pleural fluid levels of ADA activity and IFN-γ concentrations were significantly higher in TPE than NTPE (p<0.01). 2) IFN-γ production in TPE cells stimulated by PPD (755,266±886,636 pg/ml) was significantly higher than NTPE cells (3,509±6,980 pg/ml) (p<0.01). By considering the fact that IFN-γ concentrations over 10,000 pg/ml is a criteria for the diagnosis of TBE, sensitivity and specificity of the test were 97.4 and 92.3%, respectively. 3) The ratios of IFN-γ production by the stimulation with PPD and PHA (PPD/PHA) were significantly higher in TPE cells (59±85) than NTPE cells (5±18)(p<0.01). Considering the criteria for the diagnosis of TBE as PPD/PHA ratio over 5, sensitivity and specificity of the test were 76.9 and 92.3%, respectively. Conclusion : Pleural fluid cell IFN-γ production assay may be useful for the diagnosis of tuberculous pleurisy. (Tuberc Respir Dis 2005; 59: 186-192)
- keywords
- Tuberculosis, Pleurisy, PPD, IFN-γ, Tuberculosis, Pleurisy, PPD, IFN-γ
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