BCG 예방접종을 받은 개체에서 유도되어 있는 결핵균 균체항원에 특정한 CD8+T 세포의 보호 면역반응
M.tuberculosis Somatic Antigen Specific CD8+T cell Responses in BCG-Vaccinated Subjects
조성애 (연세대학교)
조상래 (연세대학교)
이경화 (한림대학교)
박승규 (국립마산결핵병원)
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초록
배 경 : 결핵의 보호면역 반응에서 CD8+T 세포에 의한 여러 기전이 중요한 역할을 한다는 사실이 최근에 보고되고 있다. IFN-γ 분비 외에도 결핵균으로 감염된 세포에 독성을 나타내어 직접 결핵균으로 감염된 세포를 제거하는 독성능 또한 그 역할이 중요하다고 알려지고 있는데, BCG 예방접종을 받은 개체에서도 이러한 균체항원에 특정한 CD8+T 세포의 독성능이 유도되어 있어서 보호면역 반응에서의 역할을 하는지 연구하였다.대상 및 방법 : HLA-A*0201 과 A*0206를 표현하며 BCG 예방접종을 한 개체들의 혈액에서 백혈구를 분리하고 균체항원의 항원결정기 (ThyA30-38) 에 대한 독성능과 ex vivo IFN-γ 분비능을 유도하였다.
- keywords
- CD8+T cells, cytotoxicity, M. tuberculosis somatic antigens, CD8+T cells, cytotoxicity, M. tuberculosis somatic antigens
Abstract
Background : The immune responses mediated by CD8+T cells are known to be significant in controlling M. tuberculosis infections. In order to determine the role of cytotoxic CD8+T cells in the protective immune mechanism in latently infected subjects, this study examined whether or not the cytotoxic immune responses of CD8+T cells specific to the M. tuberculosis somatic antigens are induced in BCG vaccinated healthy subjects. Methods : Cytotoxicity and IFN-γ elispot assays were used to investigate the activities of CD8+T cells specific for the thyA30-38 peptide epitope in circulating peripheral blood mononuclear cells (PBMC) from BCG-vaccinated HLA-A*0201 and A*0206 subjects. Results : The results indicate the cytotoxic and IFN-γ immune responses of CD8+T cells specific for thyA30-38 were induced in BCG vaccinated healthy subjects. Conclusion : The cytotoxic and IFN-γ responses by CD8+T cells specific for the M. tuberculosis somatic antigens are induced in BCG-vaccinated subjects, and appear to be involved in the protective immune mechanism in latently infected people against a M. tuberculosis infection. (Tuberc Respir Dis 2005; 59: 272-278)
- keywords
- CD8+T cells, cytotoxicity, M. tuberculosis somatic antigens, CD8+T cells, cytotoxicity, M. tuberculosis somatic antigens
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