바로가기메뉴

본문 바로가기 주메뉴 바로가기

Pretreatment of Diltiazem Ameliorates Endotoxin-Induced Acute Lung Injury by Suppression of Neutrophilic Oxidative Stress

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2006, v.60 no.4, pp.437-450






  • Downloaded
  • Viewed

Abstract

Background : Acute respiratory distress syndrome (ARDS) is characterized by severe inflammatory pulmonary edema of unknown pathogenesis. To investigate the pathogenesis of ARDS associated with neutrophilic oxidative stress, the role of phospholipase A2 (PLA2) was evaluated by the inhibition of calcium channel.Methods : In Sprague-Dawley rats, acute lung injury (ALI) was induced by the instillation of E.coli endotoxin (ETX) into the trachea. At the same time, diltiazem was given 60 min prior to tracheal instillation of ETX. Parameters of ALI such as lung and neutrophil PLA2, lung myeloperoxidase (MPO), BAL neutrophils, protein, surfactant were measured. Production of free radicals from neutrophils was measured also. Morphological studies with light microscope and electron microscope were carried out and electron microscopic cytochemistry for detection of free radicals was performed also.Results : Diltiazem had decreased the ALI parameters effectively in ETX given rats and decreased the production of free radicals from neutrophils and lung tissues. Morphological studies denoted the protective effects of diltiazem.Conclusion : Diltiazem, a calcium channel blocker, was effective in amelioration of ALI by the suppression of neutrophilic oxidative stress mediated by PLA2 activation. (Tuberc Respir Dis 2006; 60: 437-450)

keywords
ARDS, PLA2, Free Radicals, Neutrophils, ARDS, PLA2, Free Radicals, Neutrophils

Reference

1.

Ashbaugh DG, (1967) Acute respiratory distress in adults,

2.

Connelly KG, Markers for predicting the development of acute respiratory distress syndrome,

3.

Jain A, Glutathione metabolism in the lung inhibition of its synthesis leads to lamellar body and mitochondrial defects,

4.

Matthay MA, (1999) Conference summary:Acute lung injury,

5.

Fujishima S, (1995) Neutrophil-mediated tissue injury and its modulation,

6.

Pittet JF, (1997) Biological markers of acute lung injury:prognostic and pathogenetic significance,

7.

Pruzanski W, (1991) Phospholipase A2-a mediator between proximal and distal effectors of inflammation,

8.

Koh T, (1995) Cytokine induced neutrophil chemoattratant is necessary for interleukin-1 induced lung leak in rats,

9.

Hybertson BM, (2003) Transforming growth factorβ contributes to lung leak in rats given interleukin-1 intracheally,

10.

Lee YM, (1997) Mepacrine decreases lung leak in rats given interleukin-1 intratracheally,

11.

Lee YM, (2000) Platelet-activating factor contributes to acute lung leak in rats given interleukin-1 intratracheally,

12.

Lee YM, (1999) PAF contributes to intestinal ischemia reperfusion induced acute lung injury through neutrophilic oxidative stress,

13.

Canham EM, (1983) Mepacrine but not methylprednisolone decreases acute edematous lung injury after injection of phorbol myristate acetate in rabbits,

14.

Crystal RG, (1997) The lung,

15.

Viejo A, (2002) Oxidative stress in critically ill patients,

16.

Partrick DA, (2001) Secretory PLA2 activity correlates with postinjury multiple organ failure,

17.

Nagase T, (2000) Acute lung injury by sepsis and acid aspiration:a key role for cytosolic phospholipase A2,

18.

Vernon LP, (1992) Membrane structure,toxins and phospholipase A2 activity,

19.

Kwon YS, (2002) Changes in phospholipase A2 activity in the pulmonary oxidative stress of the platelet-activating factor-induced acute lung injury, Daegu catholic University

20.

Katsumata M, (1986) Rapid assay for activity of phospholipase A2 using radioactive substrate,

21.

Haslett C, (1985) Modulation of multiple neutrophil functions by preparative methods or trace concentration of bacterial lipopolysaccharides,

22.

Goldblum SE, (1985) Lung myeloperoxidase as a measurement of leukostasis in rabbits,

23.

Botha AJ, (1995) Post injury neutrophil priming and activation state:therapeutic challenges,

24.

Brown RE, (1989) Protein measurement using bicinchoninic acid:elimination of interfering substance,

25.

Bligh EG, (1959) A rapid method of lipid extraction and purification,

26.

Hess HH, (1975) Assay of inorganic and organic phosphorus in the 0.1-5 nanomolar range,

27.

Corbet A, (1983) Distension produced phospholipid secertion in postmortem in situ lungs of normal rabbits,

28.

Hobson J, (1991) Histochemical evidence for generation of active oxygen species on the apical surface of cigarette smoke exposed tracheal explants,

29.

Pruzanski W, (1993) Secretory non- pancreatic groupⅡphospholipase A2:role in physiologic and inflammatory process,

30.

Lee YM, (2002) Platelet-activating factor induces lung inflammation and leak in rats:Hydrogen peroxide production along neutrophil-lung endothelial cell interfaces,

31.

Sun X, (1994) Tumor necrosis factor and endotoxin synergistically activate intestinal phospholipase A2 in mice.Role of endogenous platelet activating factor and effect of exogenous platelet activating factor,

32.

Kramer RM, (1997) Structure, function and regulation of Ca2+-Sensitive cytosolic phospholipase A2 (PLA2).,

33.

Nakashima S, (1989) Mechanism of arachidonic acid liberation in platelet-activating factor stimulated human polymorphonuclear neutrophils,

34.

Baker DG, (jimmunol1989) stimulates human neutrophil aggregation and release of lysosomal enzymes,

35.

Koike K, (1995) Gut Phospholipase A2 mediates neutrophil priming and lung injury after mesenteric ischemia-reperfusion,

36.

Dana R, (1998) Essential requirement of cytosolic phospholipase A2 for activation of the phagocyte NADPH oxidase,

37.

Delclaux C, (1997) Alveolar neutrophils in endotoxin-induced and bacteria-induced acute lung injury in rat,

38.

Roh WS, (2001) Inhibition of phospholipase A2 ameliorates the acute lung injury induced by E coli endotoxin via reduced production of oxygen free radicals in the lung,

39.

Tsukahara Y, (1999) Pholpholipase A2 mediates nitric oxide production by alveolar macrophages and acute lung injury in pancreatitis,

40.

Nakos G,, (1998) Bronchoalveloar lavage fluid characteristic of early intermediate and late phase of ARDS Alterations in leukocytes,

41.

Martensson J, (1991) Inhibition of glutathione synthesis in the newborn rabbit:A model for endogenously produced oxidative stress,

42.

McIntosh JC, (1996) Surfactant proteins A and D increase in response to intratracheal lipopolysaccharide,

43.

Lee YM, (1996) Effects of high dose of dexamethasone on PLA2,GGT activity and lung morphology in NNNMU-induced ARDS rats,

44.

Ortolani O, (2000) Protective effects of N-acetylcysteine and rutin on the lipid peroxidation on the lung epithelium during the adult respiratory distress syndrome,

45.

Koike K, (2000) Group II phospholipase A2 mediates lung injury in intestinal ischemia-reperfusion,

Tuberculosis & Respiratory Diseases