미세분진이 흰쥐의 폐포대식세포에서 TNF-α와 IL-1β의 형성에 미치는 효과
The Effects of Air-borne Particulate Matters on the Alveolar Macrophages for the TNF-α and IL-1β Secretion
이수진 (대구교육대학교)
장병준 (건국대학교)
김길수 (경북대학교)
이명헌 (국립수의과학검역원)
최농훈 (건국대학교)
박세종 (건국대학교)
이종환 (건국대학교)
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Abstract
Background: PM is known to induce various pulmonary diseases, including asthma, cancer, fibrosis and chronic bronchitis. Despite the epidemiological evidence the pathogenesis of PM-related pulmonary diseases is unclear.Methods: This study examined the effects of PM exposure on the secretion of TNF-α and IL-1β in the cultured alveolar macrophages. The cultured primary alveolar macrophages were treated with the medium, PM (5~20㎍/㎠), LPS (5ng/ml), and PM with LPS for 24h and 48h respectively. ELISA was used to assay the secreted TNF-α and IL-β in the culture medium. Western blotting was used to identify and determine the level of proteins isolated from the culture cells. The cells cultured in the Lab-Tek?? chamber slides were stained with immunocytochemical stains.Results: PM induced TNF-α and IL-1β secretion in the culturing alveolar macrophages, collected from the SPF and inflammatory rats. However, the effects were only dose-dependent in the inflammatory macrophages. When the cells were co-treated with PM and LPS, there was a significant synergistic effect compared with the LPS in the both cell types.Conclusion: PM might be play an important role in the induction and/or potentiation of various lung diseases by oversecretion of TNF-α and IL-1β. (Tuberc Respir Dis 2006; 60: 554-563)
- keywords
- Particulate matter (PM), Alveolar macrophages, Tumor necrosis factor-α (TNF-α), Interleukin 1β (IL-1β).
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