바로가기메뉴

본문 바로가기 주메뉴 바로가기

IL-1Ra Elaboration by Colchicine Stimulation in Normal Human Bronchial Epithelial Cells

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2007, v.63 no.2, pp.145-153







  • Downloaded
  • Viewed

Abstract

Background: Asthma is a syndrome that is characterized by a variable degree of airflow obstruction, bronchial hyperresponsiveness, and airway inflammation. Colchicine is an inexpensive and safe medication with unique anti- inflammatory properties. IL-1Ra (Interleukin-1 receptor antagonist) mediates the anti-inflammatory effect in human inflammatory diseases, including asthma. This study examined whether IL-1Ra mediates the anti-inflammatory effect of colchicine in normal human bronchial epithelial cells (NHBE), RAW 264.7 cells (murine macrophage cell line), and a mouse lung. Methods: NHBE, RAW 264.7 cells and BALB/c mice were stimulated with colchicine, and the increase in the IL-1Ra level was estimated by ELISA, Western analysis and RT-PCR analysis. Results: Colchicine stimulated NHBE and RAW 264.7 cells to release IL-1Ra into the supernatant in a dose-and time-dependent manner. The major isoform of IL-1Ra in NHBE and RAW 264.7 cells is type I icIL-1Ra, and sIL-1Ra, respectively. IL-1Ra up-regulation was blocked by PD98059, a specific inhibitor in MAPK pathways. Colchicine also stimulated the secretion of IL-1Ra into the bronchoalveolar lavage (BAL) fluid of BALB/c mouse. Conclusion: Colchicine stimulates an increase in the IL-1Ra level both in vivo and in vitro, and might have an anti-inflammatory effect. (Tuberc Respir Dis 2007; 63: 145-153)

keywords
Colchicine, Asthma, IL-1Ra, MAPK., Colchicine, Asthma, IL-1Ra, MAPK.

Reference

1.

(2001) Asthma,

2.

(1998) Inflammatory cell distribution within and along asthmatic airways,

3.

(1996) Alveolar tissue inflammation in asthma,

4.

(1998) Airway inflammation in mild intermittent and in persistent asthma,

5.

(jallergyclinimmunol1990;85) A clinical and immunologic study of colchicines in asthma,

6.

(1995) Effects of colchicines on IgE-mediated early and late airway reactions,

7.

(1998) Interleukin-1 receptor antagonist: role in biology,

8.

(eurjpharmacol1992;213) Effect of interleukin-1 receptor antagonist on antigen-induced pulmonary responses in guinea pigs,

9.

(1995) Potential role of interleukin-1 in allergen-induced late asthmatic reactions in guinea pigs: suppressive effect of interleukin-1 receptor antagonist on late asthmatic reaction,

10.

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol- chloroform extraction,

11.

(1997) Extracellular release of the type I intracellular IL-1 receptor antagonist from human airway epithelial cells: differential effects of IL-4, IL-13, IFN-gamma, and corticosteroids,

12.

(eurjimmunol1993;23) Cytokine regulation of the interleukin-1 receptor antagonist protein in U937 cells,

13.

(1995) Cloning and characterization of a new isoform of the interleukin receptor antagonist,

14.

(amjmed1970;48) implications as to pharmacology and mechanism of action,

15.

(clinexprheumatol1989;7) Mechanism of action of colchicine Effect of colchicine on generation of leukotriene B4 by human polymorphonuclear leukocytes,

16.

(jimmunol1986;136) Colchicine inhibits ionophore- induced formation of leukotriene B4 by human neutrophils the role of microtubules,

17.

(agentsactions1991;32) A comparison of the anti-inflammatory activity of selective 5-lipoxygenase inhibitors with dexamethasone and colchicine in a model of zymosan induced inflammation in the rat knee joint and peritoneal cavity,

18.

(jclininvest1972;51) Histamine release from human leukocytes studies with deuterium oxide,

19.

(jclininvest1977;60) Evidence that cyclic nucleotides modulate the immunologic release of mediators through effects on microtubular assembly,

20.

Effects of colchicines on cyclic AMP levels in human leukocytes,

21.

Modulation of adenylate cyclase in intact macrophages by microtubules Opposing actions of colchicine and chemotactic factor,

22.

(immunolallergypract1992;14) Low asthma prevalence found in FMF,

23.

(jasthma1994;31) A clinical study of colchicines in childhood asthma,

24.

(1997) Failure of colchicines to reduce inhaled triamcinolone dose in patients with asthma,

25.

(genomics1993;15) gene is located in the chromosome 2q14region,

26.

(1998) The differential production of three forms of IL-1 receptor antagonist by human neutrophils and monocytes,

27.

(1998) Detection of an interleukin-1 intracellular receptor antagonist mRNA variant,

28.

(jimmunol1992;149) Differing requirements for tumor necrosis factor-α and IL-1,

29.

(1996) Regulatory effects of endogenous interleukin-1 receptor antagonist protein in immunoglobulin G immune complex-induced lung injury,

30.

(1999) Balance between proinflammatory cytokines and their inhibitors in bronchial lavage from patients with status asthmaticus,

31.

(2001) Mammalian MAP kinase signaling cascades,

32.

(2005) Mitogen-activated protein kinases and asthma,

Tuberculosis & Respiratory Diseases