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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

Platelet-activating Factor에 의한 급성폐손상에서 호중구성 산화성 스트레스에 관여하는 Cytosolic Phospholipase A2 활성도의 변화

Cytosolic Phospholipase A2 Activity in Neutrophilic Oxidative Stress of Platelet-activating Factor-induced Acute Lung Injury

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2007, v.63 no.6, pp.497-506
권영식 (대구가톨릭의대)
현대성 (대구가톨릭대학교)
이영만 (대구가톨릭대학교)
  • 다운로드 수
  • 조회수

초록

연구배경: 급성호흡곤란증후군의 병인론에 관여하는 PAF의 역할이 다양하고 중요하므로 본 연구에서는 PAF의 또 다른 작용의 가능성, 즉 cPLA2의 활성화(retrograde activation of cPLA2 by PAF)의 가능성을 검사하고자 하였다. 즉, cPLA2의 활성화에 따른 염증성 지질분자의 생성이 산소기의 생성과정을 증폭시키고 이 때 생성된 PAF가 역으로 cPLA2를 활성화시키는지를 확인하기 위하여 본 연구는 고안되었다.방 법: 흰쥐에서 급성폐손상을 유도하기 위하여 5μg의 PAF를 0.5 ml의 0.25% bovine serum albumin 용액과 혼합한 뒤 기도 내로 직접 분무하거나 0.5 ml의 4.5 mM의 과산화수소를 기도 내로 분무하였다. 대조군의 경우는 0.5 ml의 생리적 식염수를 기도 내로 분무하였다. 5 시간 후에 단백누출지수 측정, 폐장의 MPO 활성도 측정, 폐포세척액 내의 호중구 산정, CINC 측정, NBT 및 cytochrome-c 환원검사를 시행하였다. 또한 폐장 및 호중구에서의 cPLA2 활성도의 측정 및 광학현미경과 전자현미경을 이용하여 형태학적 관찰을 시행하였다.결 과: PAF투여 후 단백누출지수, MPO, BAL내의 호중구의 수 및 CINC의 농도가 대조군에 비하여 유의하게 증가하였다. NBT및 cytochrome-c환원검사의 결과 PAF는 호중구의 respiratory burst를 현저히 증가시키고, 분리된 사람의 호중구에서도 산소기의 생성을 현저히 증가시켰다. 동시에 PAF는 분리된 호중구 및 폐장의 cPLA2의 활성도도 증가 시켰다. 폐장 내로 투여한 과산화수소는 폐장의 cPLA2활성도를 대조군에 비하여 현저히 증가시켰다.

keywords
Acute lung injury, PAF, cPLA2, Neutrophils, Acute lung injury, PAF, cPLA2, Neutrophils

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Tuberculosis & Respiratory Diseases