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ISSN : 0376-4672
mutans (S.mutans ), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in 30 ㎖ of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. 1.8 ㎖ of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and 0.05 ㎎/㎖, was mixed with 0.2 ㎖ of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 ㎚ LED was used to expose light for 5 minutes to irradiate 59 ㎻ energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with 0.05㎎/㎖ curcuma powder extract, the concentration of 0.5 ㎎/㎖ and LED light of 405 ㎚ wavelength (p=0.000, p=0.003). 0.05 ㎎/㎖ of curcuma powder extract and the concentration of 0.5 ㎎/ ㎖ showed 100% antibacterial effect when exposed to light, whereas the concentration of 5 ㎎/㎖ showed 11.95% antibacterial effect. When exposed to light, 0.05 ㎎/㎖ of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of 0.5 ㎎/㎖ and 5 ㎎/㎖ did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans , no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.
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