open access
메뉴ISSN : 0376-4672
Purpose: The aim of this study was to evaluate the red fluorescence characteristics of bacterial dental deposits assessed by quantitative light-induced fluorescence (QLF) technology and confirm whether the red fluorescence can distinguish and evaluate quantitatively accumulation of bacterial dental deposits. Methods: This retrospective cross-sectional study used QLF images captured at a dental clinic from January to December 2016. In each QLF image, a skilled examiner selected one region where the presence of deposits was suspected. Then, the regions were classified into three groups of not detectable deposits(ND), half detectable deposits (HD), and full detectable deposits (FD) by two examiners according to classification criteria. Only those images where the regions of bacterial dental deposits were classified identically by all examiners were used for analysis. The mean red fluorescence intensity (RFI) was defined as the mean value of R/G for all pixels in the regions. The RFI was compared between groups using Welch’s ANOVA test, and the Spearman correlation was calculated to assess the association between RFI and accumulation of deposits. Results: In this study, 351 images among the collected images of 605 subjects were finally selected. The mean age of subjects was about 44 years. The R/G values of the ND, HD and FD were 0.73, 1.26 and 1.83 respectively. There were significant differences between all groups (p<0.001), and strong positive correlation was identified between the R/G value and the accumulation of deposits (r = 0.90, p<0.001). Conclusion: The intensity of red fluorescence as observed in the QLF images correlated well with the accumulation maturation of the deposits, which indicates that the QLF technology can be used to evaluate the status of oral hygiene.
1. Marsh PD. Dental plaque as a biofilm and a microbial community - implications for health and disease. BMC Oral Health 2006; 6 Suppl 1:S14
2. Pretty IA, Edgar WM, Smith PW, Higham SM. Quantification of dental plaque in the research environment. J Dent 2005; 33(3):193-207
3. Carter K, Landini G, Walmsley AD. Automated quantification of dental plaque accumulation using digital imaging. J Dent 2004; 32(8):623-628
4. Liu Z, Gomez J, Khan S, et al. Red fluorescence imaging for dental plaque detection and quantification: pilot study. J Biomed Opt 2017; 22(9):1-10
5. Coulthwaite L, Pretty IA, Smith PW, et al. The microbiological origin of fluorescence observed in plaque on dentures during QLF analysis. Caries Res 2006; 40(2):112-116
6. K. HC, E. dJdJ, T. FMR, et al. Photobleaching of red fluorescence in oral biofilms. Journal of Periodontal Research 2011; 46(2):228-234
7. Konig K, Flemming G, Hibst R. Laser-induced autofluorescence spectroscopy of dental caries. Cellular and Molecular Biology 1998; 44(8):1293-1300
8. Lee H-S, Kim S-K, Park S-W, et al. Caries detection and quantification around stained pits and fissures in occlusal tooth surfaces with fluorescence. Journal of biomedical optics 2018; 23(9):091402
9. Kim H-E, Kim B-I. Analysis of orange/red fluorescence for bacterial activity in initial carious lesions may provide accurate lesion activity assessment for caries progression. Journal of Evidence Based Dental Practice 2017; 17(2):125-128
10. Han SY, Kim BR, Ko HY, et al. Assessing the use of Quantitative Light-induced Fluorescence-Digital as a clinical plaque assessment. Photodiagnosis Photodyn Ther 2016; 13:34-39
11. Khudanov B, Jung HI, Kahharova D, et al. Effect of an oral health education program based on the use of quantitative light-induced fluorescence technology in Uzbekistan adolescents. Photodiagnosis and photodynamic therapy 2018; 21:379-384
12. Kim YS, Lee ES, Kwon HK, Kim BI. Monitoring the maturation process of a dental microcosm biofilm using the Quantitative Light-induced Fluorescence-Digital (QLF-D). J Dent 2014; 42(6):691-696
13. Lee ES, Kang SM, Ko HY, et al. Association between the cariogenicity of a dental microcosm biofilm and its red fluorescence detected by Quantitative Light-induced Fluorescence-Digital (QLF-D). J Dent 2013; 41(12):1264-1270
14. Alaluusua S, Malmivirta R. EARLY PLAQUE ACCUMULATION - A SIGN FOR CARIES RISK IN YOUNG-CHILDREN. Community Dentistry and Oral Epidemiology 1994; 22(5):273-276
15. Harada K, Raigrodski AJ, Chung K-H, et al. A comparative evaluation of the translucency of zirconias and lithium disilicate for monolithic restorations. The Journal of prosthetic dentistry 2016; 116(2):257-263
16. Marsh PD, Bradshaw DJ. Dental plaque as a biofilm. J Ind Microbiol 1995; 15(3):169-175
17. Wade WG. The oral microbiome in health and disease. Pharmacological research 2013; 69(1):137-143
18. van der Veen MH, Thomas RZ, Huysmans MC, de Soet JJ. Red autofluorescence of dental plaque bacteria. Caries Res 2006; 40(6):542-545
19. Volgenant CMC, Zaura E, Brandt BW, et al. Red fluorescence of dental plaque in children —A cross-sectional study. Journal of Dentistry 2017; 58:40-47
20. Lee E-S, De Jong EDJ, Jung H-I, Kim B-I. Red fluorescence of dental biofilm as an indicator for assessing the efficacy of antimicrobials. Journal of biomedical optics 2018; 23(1):015003
21. van der Veen MH, Volgenant CMC, Keijser B, et al. Dynamics of red fluorescent dental plaque during experimental gingivitis—A cohort study. Journal of Dentistry 2016; 48:71-76
22. Akcalı A, Lang NP. Dental calculus: the calcified biofilm and its role in disease development. Periodontology 2000 2018; 76(1):109-115
23. Lee Y-K. Fluorescence properties of human teeth and dental calculus for clinical applications. Journal of Biomedical Optics 2015; 20(4):040901
24. Buchalla W, Lennon AM, Attin T. Fluorescence spectroscopy of dental calculus. J Periodontal Res 2004; 39(5):327-332