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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

退行性關節炎 치료제 개발을 위한 수종의 한약재활성 검색 및 기전연구

Oriental Medicine Research Center for Bone & Joint Disease

대한한의학회지 / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2006, v.27 no.1, pp.229-239
허정은 (경희대학교부속한방병원)
조은미 (경희대학교부속한방병원)
양하루 (경희대학교부속한방병원)
김대성 (경희대학교부속한방병원)
백용현 (경희대학교부속한방병원)
이재동 (경희대학교부속한방병원)
최도영 (경희대학교부속한방병원)
박동석 (경희대학교부속한방병원)
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Abstract

Objective: Articular cartilage is a potential target for drugs designed to inhibit the activity of matrix metalloproteinases (MMPs) to stop or slow the destruction of the proteoglycan and collagen in the cartilage extracellular matrix. The purpose of this study was to investigate the effects of KHBJs for cartilage-protective effect in human and rabbit articular cartilage explants. Methods:The cartilage-protective effects of KHBJ were evaluated by using glycosaminoglycan degradation assay, collagen degradation assay, colorimetric analysis of MMPs activity, and histological analysis in rabbit and human cartilage explants culture. Results: KHBJs significantly inhibited GAG and collagen release of rabbit and human cartilage explant in a concentration-dependent manner. Also, KHBJs inhibited MMP-3 and MMP-13 activities from IL-1a-treated cartilage explants cultures. Histological analysis indicated that KHBJ004 reduced the degradation of the cartilage matrix compared with that of IL-1α-treated cartilage explants. KHBJ004 had no harmful effect on chondrocytes viability or cartilage morphology in cartilage explants. Conclusion : These results indicate that KHBJs inhibits the degradation of proteoglycan and collagen through the downregulation of MMP-3 and MMP-13 activities without affecting the viability or morphology of IL-1α-stimulated rabbit and human articular cartilage explants.

keywords
Cartilage, proteoglycan, collagen, matrix metalloproteinase, KHBJs, Cartilage, proteoglycan, collagen, matrix metalloproteinase, KHBJs


참고문헌

1

(2003) Osteoarthritis,

2

(2001) The role of bone in osteoarthritis,

3

(2000) Novel strategies for the treatment of osteoarthritis,

4

(2003) Osteoarthritis: understanding the pathophysiology, genetics, and treatments,

5

(2001) Local factors in osteoarthritis,

6

(2000) The role of complementary and alternative medicine,

7

(1986) Copeman's Textbook of the Rheumatic Diseases, ed Churchill Livingstone

8

(2000) Traditional chinese medicine:an approach to scientific proof and clinical validation,

9

(2001) Effect of SKI 306X in patients with osteoarthritis of the knee,

10

(1996) Effect of a proprietary herbal medicine on the relief of chronic arthritic pain:double blind study,

11

(2001) Herbal medicines for the treatment of osteoarthritis,

12

(1994) 025% as monotherapy for osteoarthritis, Capsacin cream 0

13

(2004) Protective and anti-arthritic effects of deer antler aqua-acupuncture(DDA) inhibiting dihydroorotate dehydrogenase, on phosphate ions-mediated chondrocyte apoptosis and rat collagen-induced arthritis,

14

(2002) Matrix metallo proteinase in arthritic disease,

15

(1999) Inhibition of interleukin-1-induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product Journal of Pharmacology and Experimental Therapeutics 1999,

16

(2002) Effects of SKI306X on proteoglycan degradation in cartilage explant culture and collagenase-induced rabbit osteoarthritis model,

17

(1989) Studies on interleukin-1b induced glycosaminoglycan release from rat femoral head cartilage in-vitro Journal of Pharmacy and Pharmacology 1989,

18

(2003) Glucosamin sulfate modulates the levels of aggrecan and matrix metalloproteinase-3 synthesized by cultured human osteoarthritis articular chondrocytes,

19

(1990) There are two major types of skeletal keratan sulphates,

20

(1992) Localization of matrix metalloproteinase 3,

21

(1992) Collagen degradation induced by the combination of IL-1alpha and plasminogen in rabbit articular cartilage explant culture Journal of Biochemistry,

22

(1998) Involvement of MMP-1 and MMP-3 in collagen degradation induced by IL-1 in rabbit cartilage explant culture,

23

(2000) Retinoic acid combines with interleukin-1 to promote the degradation of collagen from bovine nasal cartilage Matrix Metalloproteinase-1 and 13 are involved in cartilage breakdown Journal of Cellular Biochemistry 2000,

24

(1996) Cytokines in osteoarthritis Mediators on markers of joint destruction Seminars in Arthritis and Rheumatism 1996,

25

(1991) Jr Matrix metalloproteinases and their inhibitors in connective tissue remodelling,

26

(2000) Osteoarthritis: New insights,

27

(2000) Osteoarthritis: New insights,

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