- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Effect of Yikgeebohyul-tang (Yìqìbǔxuè-tāng) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2010, v.31 no.1, pp.138-152
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(2010). Effect of Yikgeebohyul-tang (Yìqìbǔxuè-tāng) on Hair Regrowth and Cytokine Changes on Hair-Removed C57BL/6 Mice. Journal of Korean Medicine, 31(1), 138-152.
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Abstract
Objective: Yikgeebohyul-tang (Yìqìb xuè-tang) is one of the representative prescriptions for invigoration of vitality and nourishing of the blood. This study was carried out to investigate the effects of Yikgeebohyul-tang (Yìqìb xuè-tang)on hair regrowth and cytokine changes in a shaving model of C57BL6 mice. Method: Five-week-old mice were acclimated for 1 week at a temperature between 21-23 É, 40-60% relative humidity, and 12h of a light/dark cycle before beginning of the experiment. There were two groups including normal saline (control) and a positive control of oral intake of Yikgeebohyul-tang (Yìqìb xuè-tang) extract (sample) in 18female mice. The test compounds were topically treated once a day over 14 days. The hair regrowth was photographically and histologically determined during the experimental period of 14 days. Revelation of TGF- 1 and β EGF were also determined using immunohistochemistry. In addition to that, IFN- , IL-4 and IL-10 were determined γ in serum. Results: Hair regrowth in the sample group was promoted earlier and faster than the control group, as shown by concentrations of hairs and thick-hair ratio in the sample group. TGF- 1 was not revealed in either control orsample β group. EGF was strongly positive in out root sheath of some thick hair of the sample group. Serum IFN- was γ significantly decreased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14 experimental days. Serum IL-4 was significantly increased in the sample group compared with the control group at 7 experimental days. However, it was not significantly decreased at 14experimental days. Serum IL-10 was decreased in the sample group compared with the control group, but with no real statistical significance. Conclusion: These results suggest that Yikgeebohyul-tang (Yìqìb xuè-tang) has hair growth promoting activity and it can be used for treatment of alopecia. Also, these effects relate to EGF revelation of hair roots, a decrease in serum IFN- , and an increase of serum IL-4.
- keywords
- Alopecia, C57BL/6 mice, Yikgeebohyul-tang, hair regrowth
Reference
1
1 Otomo S. new hair growth drugs and their nonclinical evaluation methods. Hyangjanghwoiji. 1997;21:228.
2
2 Kagoura M, Morohashi M, Ogita Z. Animal models for the study of alopecia. Hyangjanghwoiji. 1997;21:207.
3
3 Chao YF. Zhubingyuanhoulun. Beijing:People's Medical Publishing House. 1983;761-7.
4
4 Lim SBN, Choi KD, Kim SK. The Oriental and Western Medical Study of Alopecia. The Journal of Jeahan Oruental Medical Academy. 1999;4(1):699-710.
5
5 Meng X, Sawamura D, Tamai K, Hanada K, Ishida H, Hashimoto I. Keratinocyte gene therapy for systemic diseases. Circulating interleukin-10 released from gene-transferred keratinocytes inhibits contact hypersensitivity at distant areas of the skin. J Clin Invest. 1998;101(6):1462-7.
6
6 Gilhar A, Landau M, Assy B, Ullmann Y, Shalaginov R, Serafimovich S, et al. Transfer of alopecia areata in the human scalp graft/ Prkdc(scid) (SCID) mouse system is characterized by a TH1 response. Clin Immunol. 2003;106(3):181-7.
7
7 Tanyasiri K, Hira K, Mitsuishi K, Ueki R, Sekigawa I, Ogawa H. Interleukin-16 in patients with alopecia areata. J Dermatol Sci. 2005;37 (1):55-7.
8
8 Kim JH, Lee TH. Effects of Bojungikgitang on Starvation stress in Mice. The Korean Journal of Oriental Medical Prescription. 2006;14(1):133-40.
9
9 Kim SM, Sim SY, Byun HS, Kim KJ. The Effect of Bojungikgi-tang in mouse model of Allergic rhinitis. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2005;18(3):26-36.
10
10 Baek TH, Jee HC. Study on effect of Bojungikgitang affecting lactate tolerance and recovery rate. Journal of Oriental Rehabilitation Medicine. 1998;8(1):171-86.
11
11 Park SD, Seo GS, Park WH. Effects of Bojungikgitang, Soeumin Bojungikgi-tang and its component groups on Antioxidant Activities in alloxaninduced diadetic rats. Kor. J. Herbology. 2001;16 (2):113-26.
12
12 Kim JS, Sim SW, U YS, Nam HJ, Kim KJ. The Effect of Bojungikgi-tang on the NAsal tissue in allergic rhinitis of ovalbimin-inhalation Rats. Journal of the oriental medical surgery, ophthalmology & otolaryngology. 2004;17(1):66-74.
13
13 Eun JS, Choi H, Song JM. Effect of Bojungikgitang on the Specific immune Response in BALB/c Mice. Korean J. Oriental Physiology & Pathology. 2002;16(5):943-7.
14
14 Gong TX. Wanbinghuichun. Seoul:Haenglim publishing company. 1972;220-2.
15
15 Heo J. Dongeuibogam. Seoul:Namsandang. 1983 ;202-3,359.
16
16 Chen SW. Taipinghuiminhejijufang. Beijing:People's Medical Publishing House. 1985;179-80, 310-1.
17
17 Yang YG. Huangdineijingsuwenyijie. Seoul:Seongbosa 1980;5-10,93-4,337,340.
18
18 Yang YG. Huangdineijinglingshuyikjie. Seoul: Seongbosa. 1980;104,452-6.
19
19 Hirobe T. Structure and function of melanocytes: microscopic morphology and cell biology of mouse melanocytes in the epidermis and hair follicle. Histol Histopathol. 1995;10(1):223-37.
20
20 Li J, Tzu J, Chen Y, Zhang YP, Nguyen NT, Gao J, et al. Laminin-10 is crucial for hair morphogenesis. EMBO J. 2003;22(10):2400-10.
21
21 Peters EM, Hansen MG, Overall RW, Nakamura M, Pertile P, Klapp BF, et al. Control of human hair growth by neurotrophins: brain-derived neurotrophic factor inhibits hair shaft elongation, induces catagen, and stimulates follicular transforming growth factor beta2 expression. J Invest Dermatol. 2005;124(4):675-85.
22
22 Danilenko DM, Ring BD, Yanagihara D, Benson W, Wiemann B, Starnes CO, et al. Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation. Normalization of the nu/nu follicular differentiation defect and amelioration of chemotherapy-induced alopecia Am J Pathol. 1995;147(1):145-54.
23
23 Lindner G, Botchkarev VA, Botchkareva NV, Ling G, van der Veen C, Paus R. Analysis of apoptosis during hair follicle regression (catagen). Am J Pathol. 1997;151(6):1601-17.
24
24 Paus R, Christoph T, Muller-Rover S. Immunology of the hair follicle: a short journey into terra incognita. J Invest Dermatol Symp Proc. 1999;4 (3):226-34.
25
25 Christoph T, Muller-Rover S, Audring H, Tobin DJ, Hermes B, Cotsarelis G, et al. The human hair follicle immune system: cellular composition and immune privilege. Br J Dermatol. 2000;142 (5):862-73.
26
26 Paus R, Hofmann U, Eichmuller S, Czarnetzki BM. Distribution and changing density of gamma-delta T cells in murine skin during the induced hair cycle. Br J Dermatol. 1994;130(3): 281-9.
27
27 Ito T, Ito N, Saathoff M, Bettermann A, Takigawa M, Paus R. Interferon-gamma is a potent inducer of catagen-like changes in cultured human anagen hair follicles. Br J Dermatol. 2005;152 (4):623-31.
28
28 Bodemer C, Peuchmaur M, Fraitaig S, Chatenoud L, Brousse N, De Prost Y. Role of cytotoxic T cells in chronic alopecia areata. J Invest Dermatol. 2000;114(1):112-6.
29
29 Neville LF, Mathiak G, Bagasra O. The immunobiology of interferon-gamma inducible protein 10 kD (IP-10): a novel, pleiotropic member of the C-X-C chemokine superfamily. Cytokine Growth Factor Rev. 1997;8(3):207-19.
30
30 Miceli MC, Parnes JR. Role of CD4 and CD8 in T cell activation and differentiation. Adv Immunol. 1993;53:59-122.
31
31 Kwon GJ . Experimental Studies on Hair Growth Effect of Samultang-gamibang in Spontaneous Alopecia Areata Mice. Professional Graduate School of Oriental Medicine Wonkwang University.2004.
32
32 KIm PS, Kim HT, Roh SS, Whang CY. Effect of DanGuiBoHyulTangGami-Bang on the Alopecia and Hair Growth Stimulation. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2004;17(3):38-60.
33
33 Kim JJ . Dongyangeuihak Jinryoyogam. Seoul: The Institute of Oriental medicine. 2007;176.
34
34 Lee G. Dongyuashizhongyishu. Seoul:Daeseong medicine publishing company. 1983;35-7,86-7.
35
35 Youn GY. Dongeuiyimsangbangjehak. Seoul: Myungbo publishing company. 1987;254-63,303-8.
36
36 Lee HY, Baek TH. Effect of Jaeumgeonbi-tang Extract on Indomethacin-Induced Gastric Mucosal Lesions. J Korean Oriental Med. 2004;25(3):111-22.
37
37 Lim KM, Jeong HW. Mechanism of Jaeumgeonbitang adding Aurantii Fructus.Gastrogae Rhizoma on the Improvement and Changes of Cerabral Hemodynamics. Korean Journal of Oriental Physiology and Pathology. 2003;17(1):64-70.
38
38 Hong DS. The Effect of Jaeumgunbitang on The Brain Catecholamine Contents and Body Weight of Rats in Immobilization Stress. Graduate School Kyung Hee University. 1992.
39
39 Kim JH, Lee KS. Experimental Study on the Effects of Jaeumgeonbi-tang on Sedative Action and Hematosis. J Korean Oriental Med. 1988;9 (1):35-41.
40
40 Randall VA, Sundberg JP, Philpott MP. Animal and in vitro models for the study of hair follicles. J Investig Dermatol Symp Proc. 2003;8(1):39-45.
41
41 Powell B, Crocker L, Rogers G. Hair follicle differentiation: expression, structure and evolutionary conservation of the hair type II keratin intermediate filament gene family. Development. 1992;114(2):417-33.
42
42 Rogers GE. Hair follicle differentiation and regulation. Int J Dev Biol. 2004;48(2-3):163-70.
43
43 Kreplak L, Merigoux C, Briki F, Flot D, Doucet J. Investigation of human hair cuticle structure by microdiffraction: direct observation of cell membrane complex swelling. Biochim Biophys Acta. 2001;1547(2):268-74.
44
44 Soma T, Ogo M, Suzuki J, Takahasi T, Hibino T. Analysis of apoptotic cell death in human hair follicles. J Invest Dermatol 1998;11:948-954.
45
45 Kalish RS, Gilhar A. Alopecia areata: autoimmunitythe evidence is compelling. J Invest Dermatol Symp Proc. 2003;8:164-167.
46
46 Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth cycle. Dermatol Clin. 1996;14(4):559-72.
47
47 Jindo T, Tsuboi R, Imai R, Takamori K, Rubin JS, Ogawa H. The effect of hepatocyte growth factor/scatter factor on human hair follicle growth. J Dermatol Sci. 1995;10(3):229-32.
48
48 Mitsui S, Ohuchi A, Hotta M, Tsuboi R, Ogawa H. Gene for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicle. Br. J Dermatol. 1997;137:693.
49
49 Mak KK, Chan SY. Epidermal growth factor as a biologic switch in hair growth cycle. J Biol Chem. 2003;278(28):26120-6.
50
50 Inui S, Fukuzato Y, Nakajima T, Yoshikawa K, Itami S. Androgen-inducible TGF-beta1 from balding dermal papilla cells inhibits epithelial cell growth: a clue to understand paradoxical effects of androgen on human hair growth.FASEB J. 2002;16(14):1967-9.
51
51 Soma T, Dohrmann CE, Hibino T, Raftery LA. Profile of transforming growth factor-beta responses during the murine hair cycle. J Invest Dermatol. 2003:121(5):969-75.
52
52 Lee hs, Song JY, Yun Km, Jae YM. Hair growth effect of Mori Cortex Mixture on the hair of rat. Korean J. Seric. Sci. 2000;42(2):83-5.
53
53 Choi W, Choi JH, Kim JH. Effect on Hair Growth by various herb medicine. The Journal of Oriental Ophthalmology & Otolaryngology & dermatology. 2002;15(2):80-103.
54
54 Shin YS. Studies on the effect of five herbal extracts on hair growth activity. Dept. of Oriental MedicineGraduate School Daejeon University. 2003.
55
55 Kim MY, An BJ. A Study of Hair Loss Prevention and Hair Growth Promotion by Korean Traditional Fomular. Korean journal of herbology. 2004:19 (2);9-20.
56
56 Park WS, Sung DS, Kim DK, Cho WH, Lee HK, Lee CH, et al. The Effect of Hair Essence (HHRHG0202-80) Containing Five Herbal Extracts on Hair Growth and the Prevention of Alopecia in vitro& vivo. J Korean Oriental Med. 2004;25 (1):152-60.
57
57 Botchkarev VA, Kishimoto J. Molecular control of epithelial-mesenchymal interactions during hair follicle cycling. J Investig Dermatol Symp Proc. 2003;8(1):46-55.
58
58 Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev. 2001;81(1):449-494.
59
59 Hwang HJ, Kim JD. A Case Study on the Iron Deficiency Anemia. Korean J. Orient. Int. Med. 2000;21(4):655-60.
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