- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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The Protective Effects of Chilgi-tang on Oxidative Stress by Glucose Deprivation in Neuro 2A Cells
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2010, v.31 no.2, pp.1-18
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(2010). The Protective Effects of Chilgi-tang on Oxidative Stress by Glucose Deprivation in Neuro 2A Cells. Journal of Korean Medicine, 31(2), 1-18.
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Abstract
Objective:The water extract of Chilgi-tang (CGT) has been traditionally used in treatment of heart diseases caused by stress in Oriental Medicine. However, little is known about the mechanism by which CGT rescues neuronal cells from injury damage. Therefore, this study was designed to evaluate the protective effects of CGT on Neuro-2A cells by glucose deprivation-induced cell death. Methods: We investigated how cell death induced by glucose deprivation was associated with increased reactive oxygen species (ROS) generation. Result:The CGT treatment prior to glucose deprivation insult significantly reduced the number of cell deaths and the glucose deprivation-induced increase in ROS. Nitric oxide (NO) was also attenuated by CGT treatment. In addition, we demonstrated that the anti-cell death effect of CGT was blocked by heme oxygenase-1 (HO-1) activation. Finally, pretreatment of cells with a hemin, HO-1 inducer, reduced glucose deprivation-induced cell death. In contrast, pretreatment of cells with a ZnPP, HO-1 activity inhibitor, attenuated CGT-induced inhibition of cell death. Conclusions:These findings indicate that ROS plays an important role in glucose deprivation-induced cell death and that CGT may prevent glucose deprivation-induced cell death by inhibiting the ROS generation through HO-1 activation in Neuro-2A cells.
- keywords
- Chilgi-tang, Neuro-2A cells, glucose deprivation, oxidative stress
Reference
1
1 Moon MK, Choi BM, Oh GS, Pae HO, Kim JD, Oh H, et al. Catalposide protects Neuro 2A cells from hydrogen peroxide-induced cytotoxicity via the expression of heme oxygenase-1. Toxicol Lett. 2003;145(1):46-54.
2
2 Imuta N, Hori O, Kitao Y, Tabata Y, Yoshimoto T, Matsuyama T, et al. Hypoxia-mediated induction of heme oxygenase type I and carbon monoxide release from astrocytes protects nearby cerebral neurons from hypoxia-mediated apoptosis. Antioxid Redox Signal. 2007;9(5):543-52.
3
3 Hwang YP, Jeong HG. The coffee diterpene kahweol induces heme oxygenase-1 via the PI3K and p38/Nrf2 pathway to protect human dopaminergic neurons from 6-hydroxydopaminederived oxidative stress. FEBS Lett. 2008;582(17):2655-62.
4
4 Lee JC, Bhora F, Sun J, Cheng G, Arguiri E, Solomides CC, et al. Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury. Am J Physiol Lung Cell Mol Physiol. 2008;294(2): L255-65.
5
5 Orozco LD, Kapturczak MH, Barajas B, Wang X, Weinstein MM, Wong J, et al. Heme oxygenase-1 expression in macrophages plays a beneficial role in atherosclerosis. Circ Res. 2007;100(12):1703-11.
6
6 Cheng PY, Lee YM, Shih NL, Chen YC, Yen MH. Heme oxygenase-1 contributes to the cytoprotection of alpha-lipoic acid via activation of p44/42 mitogen-activated protein kinase in vascular smooth muscle cells. Free Radic Biol Med. 2006;40(8):1313-22.
7
7 Kim HY, Shin SH, Lee I. Neuroprotective effect of hwangryunhaedok-tang against glucosedeprivation induced cytotoxicity in PC-12 cells. Korean J. Oriental Physiology & Pathology. 2008;22(6):1462-9.
8
8 Jung SH, Lee JM, Lee CH, Cho JH, Jang JB, Lee KS, et al. Neuroprotective effect of Yukul-tang against the oxidative stress. The Journal of Oriental Obstetrics & Gynecology. 2009;22(1):15-30.
9
9 Kao TC, Shyu MH, Yen GC. Neuroprotective effects of glycyrrhizic acid and 18beta-glycyrrhetinic acid in PC12 cells via modulation of the PI3K/Akt pathway. J Agric Food Chem. 2009; 57(2):754-61.
10
10 Benmoyal-Segal L, Soreq H. Gene-environment interactions in sporadic Parkinson's disease. J Neurochem. 2006;97(6):1740-55.
11
11 Zhou M, Baudry M. EUK-207, A superoxide dismutase/catalase mimetic, is neuroprotective against oxygen/glucose deprivation-induced neuronal death in cultured hippocampal slices. Brain Res. 2009;1247:28-37.
12
12 Zhang ZG, Chopp M, Bailey F, Malinski T. Nitric oxide changes in the rat brain after transient middle cerebral artery occlusion. J Neurol Sci. 1995;128:22-7.
13
13 Suh SW, Shin BS, Ma H, Van Hoecke M, Brennan AM, Yenari MA, et al. Glucose and NADPH oxidase drive neuronal superoxide formation in stroke. Ann Neurol. 2008;64(6):654-63.
14
14 Zhang L, Deng T, Sun Y, Liu K, Yang Y, Zheng X. Role for nitric oxide in permeability of hippocampal neuronal hemichannels during oxygen glucose deprivation. J Neurosci Res. 2008;86:2281-91.
15
15 Keynes RG, Garthwaite J. Nitric oxide and its role in ischaemic brain injury. Curr Mol Med. 2004;4:179-91.
16
16 Willmot M, Gibson C, Gray L, Murphy S, Bath P. Nitric oxide synthase inhibitors in experimental ischemic stroke and their effects on infarct size and cerebral blood flow: a systematic review. Free Radic Biol Med. 2005;39:412-25.
17
17 Jiang MH, Kaku T, Hada J, Hayashi Y. Different effects of eNOS and nNOS inhibition on transient forebrain ischemia. Brain Res. 2002;946:139-47.
18
18 Choi BM, Kim HJ, Oh GS, Pae HO, Oh H, Jeong S, et al. 1,2,3,4,6-Penta-O-galloyl-beta-D-glucose protects rat neuronal cells (Neuro 2A) from hydrogen peroxide-mediated cell death via the induction of heme oxygenase-1. Neurosci Lett. 2002;328(2):185-9.
19
19 Lindsten T, Zong WX, Thompson CB. Defining the role of the Bcl-2 family of proteins in the nervous system. Neuroscientist. 2005;11(1):10-5.
20
20 Byun JS. Effects of Chilgitang on the gastroiontestinal mucosa of rats stressed by heating and immobilization. The Journal of East-West Medicines. 1998;23(1):10-20.
21
21 Shin JS, Moon JY. Effect of Chilgitang extract for herb-acupuncture on ROS, RNS, lipid peroxidation and LDL oxidation. Journal of Meridian & Acupoint. 2008;25(4):89-104.
22
22 Yoon SW. A Study on the Chronically Ill elders in a university hospital[master's thesis]. Yongin: Dankook Univ.; 2001.
23
23 Polster BM, Fiskum G. Mitochondrial mechanisms of neural cell apoptosis. J Neurochem. 2004; 90:1281-9.
24
24 Kwon GR. Effects of Pinelliae rhizoma on prevention of cortical neuronal cell death and gene expression[dissertation]. Kyeongju: Dongguk univ.; 2005.
25
25 Kang SI. Protective effect of Ginseng radix against hydrogen peroxide-induced apoptosis on SK-N-MC neuroblastoma cells[dissertation]. Seongnam: Kyungwon univ.; 2004.
26
26 Choi SW. Ginseng radix reduces ischemiainduced apoptosis and cell proliferation in the dentate gyrus of gerbils[master's thesis]. Seongnam: Kyungwon univ.; 2004.
27
27 Schulz JB, Lindenau J, Seyfried J, Dichgans J. Glutathione, oxidative stress and neurodegeneration. Eur J Biochem. 2000;267(16):4904-11.
28
28 Greene LA, Tischler AS. PC12 pheochromocytoma cells in neurobiological research. Adv. Cell Neurobiol. 1982;3:373-414.
29
29 Cha YS. Protective effects of Oyaksunkisan on oxidative stress-induced injury of Neuro 2A cells[dissertation]. Iksan: Wonkwang univ.; 2003.
30
30 Lee KS. Protective effects of Dodamtanggami on retenone-induced oxidative injury of Neuro 2A cells[master's thesis]. Iksan: Wonkwang univ.; 2004.
31
31 Lee JS. Protective effects of Ondamtang on glutamate-induced apoptosis C6 glial cells [dissertation]. Iksan: Wonkwang univ.; 2007.
32
32 Song YJ. Protective effects of Chodeungsan on retenone-induced oxidative damage of Neuro 2A cells[master's thesis]. Iksan: Wonkwang univ.; 2004.
33
33 Chen SW. Prescriptions from the Great Peace Imperial Grace Pharmacy. Beijing:China Press of Traditional Chinese Medicine. 1996:63.
34
34 Hwang DY. Compilation of Formulas and Medicinals. Seoul:Younglim Publishing Inc. 2002:219.
35
35 Jo HJ. Study on the relationship of brain and heart based on oriental medicine. Korean J. Oriental Physiology & Pathology. 2005;19(3): 1496-503.
36
36 Fujimura M, Tominaga T, Chan PH. Neuroprotective effect of an antioxidant in ischemic brain injury: involvement of neuronal apoptosis. Neurocrit Care. 2005;2(1):59-66.
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