- P-ISSN 1010-0695
- E-ISSN 2288-3339
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- P-ISSN1010-0695
- E-ISSN2288-3339
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Anti-adipogenic Effect of Kaempferol, a Component of Polygonati Rhizoma
Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2010, v.31 no.2, pp.158-166
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(2010). Anti-adipogenic Effect of Kaempferol, a Component of Polygonati Rhizoma. Journal of Korean Medicine, 31(2), 158-166.
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Abstract
Objective:It has been reported that Polygonati rhizoma (Pr) has anti-hyperglycemia, anti-triglycemia, anti-diabetic, and anti-tumor activity. Total extract of Pr was tested to identify anti-adipogenic activity in 3T3-L1 differentiation and molecular mechanism of Pr in 3T3-L1 differentiation. Methods:Differentiation of 3T3-L1 pre-adipocyte was induced in the presence of Pr extract and kaempferol. The level of lipid accumulation was measured by Oil Red O staining. The expression of genes associated with adipocyte differentiation was measured by RT-PCR. Results:Extract of Pr and its component kaempferol reduced lipid accumulation in 3T3-L1 during adipogenesis and also reduced mRNA levels of genes associated with adipogenesis, such as adipsin, aP2, LPL, SERBP-1c and PPARγ. Conclusions:In this study, we showed that the molecular mechanism of Pr and kaempferol activity is related to regulation of PPARγ expression and activation.
- keywords
- Polygonati rhizoma, kaempferol, adipogenesis, 3T3-L1 differentiation, PPARγ, SREPB-1c
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