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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

The Effects of Gamisipjeon-tang on the Skin Regeneration of Deep Second Degree Burns in Mice

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2010, v.31 no.3, pp.107-121
Hyun-Jung Yu (Dongguk Univ-Seoul)

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Abstract

Objective: This study aimed to ascertain the curative effects of Gamisipjeon-tang (GST) used for wound healing on the skin regeneration of deep second degree burns in mice. Material & Methods: In vitro, the IκB kinase (IKK) mRNA expression, inducible nitric oxide synthase (iNOS) mRNA expression, and cyclooxygenase-2 (COX-2) mRNA expression in the GST concentration from 1 ㎎/㎖ to 10 ㎎/㎖ were measured. In vivo, the mice were divided into four groups : the normal group, the BE group (burn-elicited group, control group), the DC group (Duoderm CGF-treated group after burn elicitation), and the GST group (Gamisipjeon-tang treated group after burn elicitation). To determine the anti-inflammatory effects, nuclear factor (NF)-κB p65, iNOS, COX-2 positive reaction were measured by immunohistochemistry. To estimate the skin regenerative effects, change of burn area, 5-bromo-2‘-deoxyuridine (BrdU), and fibroblast growth factor (FGF) positive reaction were analyzed. Results: In vitro, the iNOS, IKK, COX-2 mRNA expression decreased according to the increase of GST concentration. The significant decrease of COX-2, iNOS, NF-κB positive reaction were the highest in the GST group, followed by the DC group and the BE group (p<0.05). The diameter of burn area was significantly decreased in the GST group as compared to that in the DC and BE group (p<0.05). The BrdU and FGF positive reaction increased more significantly in the GST group than in the DC group, and more significantly in the DC group than in the BE group on the 3rd and 7th day after burn (p<0.05). FGF positive reaction increased in the BE and DC group, whereas it decreased significantly in the GST group on the 14th day (p<0.05). The BrdU positive reaction increased in the BE group, whereas it decreased significantly in the DC and GST group on the 14th day (p<0.05). Conclusions: This study shows that GST could decrease the inflammatory response and accelerate the skin regeneration as compared to the duoderm CGF in mice with deep second degree burns.

keywords
deep second degree burns, Gamisipjeon-tang (Jiaweishiquan-tang), duoderm CGF, iNOS, NF-κB, FGF, BrdU.


Reference

1

1 Lu K, Li H. Study on the management of postburn pathological scars. Chin J Burns. 2004; 20(2):65-66.

2

2 Wu Z. Critical aspects in the treatment of postburn scars. Chin J Burns. 2004;20(2):67-68.

3

3 Kirsner RS, Falanga V, Eaglstein WH. The biology of skin grafts: skin graft as pharmacology agents. Arch Dermatol. 1993;129:481-483.

4

4 Balusubramani M, Kumar TR, Babu M. Skin substitute: a review. Burns. 2001;27:534-544.

5

5 Korean research group for wound care. Advances in wound care. Seoul:Korea medical book. 2002:315.

6

6 Sheridan RI, Tompkins RG. Skin substitutes in burns. Burns. 1999;25:97-103.

7

7 Lee KW, Kim KH. Effects of emitted-qi therapy and cold water therapy on blood features and serum in burned rats. Kor. J. Oriental Preventive Medical Society. 2000;4(1):17-31.

8

8 Ma HS, Joo SY, Han KS, Park KH, Ahn YP. Effect of cold water therapy on the burned tissue in rats. Journal of Korean society of plastic and reconstructive surgery. 1982;9(1):31-37.

9

9 Bayat M, Vasheghani MM, Razavi N, Taheri S, Rakhshan M. Effect of low-level laser therapy on the healing of second-degree burns in rats: a histological and microbiological study. J. of photochemistry and photobiology B.:biology. 2005;78:171-177.

10

10 Bayat M, Vasheghani MM, Razavi N. Effect of low-level helium-neon laser therapy on the healing of third-degree burns in rats. J. of photochemistry and photobiology B.:biology. 2006;83:87-93.

11

11 Koo HM, Lee SM, Nam KW, Kim SB, Cheon SH, Kang JH, et al. Effects of low power laser for the expression of substance P in the burned skin of the rats. The journal of Korean society of physical therapy. 2003;15(3):239-250.

12

12 Rho MH, Yong JH. The effects of the low power wavelengths of helium-neon infra red laser on the changes of the serum immunoglobulin concentration in burn rats. The journal of Korean society of physical therapy. 2000;12(3):287-292.

13

13 Park DM, Park RH. An effect of carbon arc lamp and low level laser therapy on the changes of burned mice's cytokine. The journal of Korean society of physical therapy. 2006;18(6):13-21.

14

14 Lee SM, Koo HM, Nam KW, Kim SB, Kim J.S. The effects of low power laser for the expression of epithelial growth factor in the burned skin of the rats. The journal of Korean society of physical therapy. 2002;14(3):226-237.

15

15 Rhie SG, Park YJ. The growing effects of Mortierella alpina contained diets and burn healing effects of Mortierella alpina extracted oil in rat. Suwon university. 2000;19:285-297.

16

16 Rhie SG, Kang HY, Cho BH. Effects of arachidonate-rich oil (ARASCO) extracted from Mortierella alpina on the healing of burned rat skin. Suwon university. 2003;2(1):91-99.

17

17 Na YK, Hong HS. The effect of Ulmus dressing on burn wound on rats. J. Korean biological nursing science. 2005;7(1):89-98.

18

18 Nam SY, Lee BJ. Effect of a mixture of Ear shell ash and Sesame oil on burning in a rat model. Kor. J. Vet. Publ. Hlth. 2003;27(2):119-127.

19

19 Min DH, Kim DK, Lim HP, Yang HH. Transdermal drug delivery & therapeutic effect of the preparations of Lithospermi Radix and Gardeniae Frectus extracts on the burn & wound healing. H. Kor. Pharm. Sci. 2005;35(4):255-263.

20

20 Jung SY, Kang EK, Seo HS. One case of second degree burn wound. Journal of korean oriental medical ophthalmology & otolaryngology & dermatology. 2007;20(1):285-293.

21

21 Ku YH, Choi IH. A case of second degree burn on the feet. Journal of korean oriental medical ophthalmology & otolaryngology & dermatology. 2003;16(3):260-267.

22

22 Jeong DH, Sim SH, Choi JH. A case of treatment of 3 degree burn. Journal of korean oriental medical ophthalmology & otolaryngology & dermatology. 2002;15(2):315-325.

23

23 Chi GY. A study on histological recuperative effect of burn remedies. Korean J Oriental Physiology & Pathology. 2002;16(4):774-781.

24

24 Won SH, Owi JS, Choi EJ, Kwon KR. A clinical case study of acupuncture treatment for burned hand. The Journal of Korean Acupuncture & Moxibustion Society. 2005;22(1):13-17.

25

25 Heo J. Dong Ui Bo Gam. Seoul:Bub In. 1999: 1426.

26

26 Jung ARNR, Hong SU. Hong. A case of atopic dermatitis with nummular eczema. 2006;19(2): 296-303.

27

27 Han SH, Lee YH, Kim CW. The clinical experience of the burn patients at the seoul national university hospital burn unit. J. of korean society of plastic and reconstructive surgeons papers. 1986;13(4):481-487.

28

28 Kim SK, Park JM. Management of deep burn wound using aquacell Ag. J. of korean burn society. 2006;9(1):56-58.

29

29 Ju HS, Lim JS, Choi YS, Chung SH. Treatment of 20 burn wounds with silicone gel sheets. J. of korean burn society. 2005;8(2):220-225.

30

30 Fraser JH, Cuttle L, Kempf M, Kimble RM. Cytotoxity of topical antimicrobial agents used in burn wound in australasia. Anz J Surg. 2004; 74:139.

31

31 Cho AR. Effect of silver sulfadiazine on the skin cell proliferation and wound healing process in hairless mouse 2nd degree burn model. J. Kor Pharm Sci. 2002;32:113.

32

32 Winter GD. A note wound healing under dressings with special reference to perforated film dressing. J Invest Dermatol. 1965;45:299.

33

33 Kim YS, Chung CM, Kim HS, Seo DK, Cho WS, Sohn TK. Treatment of second degree burn wound using hydrocholloid dressings. J. of orean burn society. 2005;8(1):88-96.

34

34 Usami Y, Minami S, Okamoto Y, Matsuhashi A, Shigemasa Y. Influence of chain length of Nacetyl- D-glucosamine and D-glucosamine residues on direct and complement-mediated chemotactic activities for canine polymorphonuclear cells. Carbohydr. Polym. 1997;32:115-122.

35

35 Ueno H, Yamada H, Tanada I, Kaba N, Matsuura M, Okumura M, et al. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials. 1999;20: 1407-1414.

36

36 Lee AK, Sung SH, Kim YC, Kim SG. Inhibition of lipopolysaccharide inducible nitric oxide synthase, TNF- $\alpha$ and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 2003;139:11-20.

37

37 Hobbs AJ, Higgs A, Moncada S. Inhibition of nitric oxide synthase as a potential therapeutic target. Annu. Rev. Pharmacol. Toxicol. 1999; 39:191-220.

38

38 Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annu. Rev. Med. 2002;53: 35-57.

39

39 Shen SC, Lee WR, Lin HY, Huang HC, Ko CH, Yang LL, et al. In vitro and in vivo inhibitory activities of rutin, wogonin, and quercetin on lipopolysaccharide-induced nitric oxide and prostaglandin E2 production. Eur. J. Pharmacol. 2002;446:187-94.

40

40 Lo AH, Liang YC, Lin-Shiau SY, Ho CT, Lin JK. Carnosol, an antioxidant in rosemary, suppresses inducible nitric oxide synthase through down-regulating nuclear factor-$\kappa$B in mouse macrophages. Carcinogenesis. 2000;23:983-91.

41

41 Mayer B, Hemmens B. Biosynthesis and action of nitric oxide in mammalian cells. Trends Biochem. Sci. 1997;22:477-481.

42

42 Raghav SK, Gupta B, Shrivastava A, Das HR. Inhibition of lipopolysaccharide-inducible nitric oxide synthase and IL-1$\beta$ through suppression of NF-$\kappa$B activation by 3-(1'-1'-dimethyl-allyl)- 6-hydroxy-7-methoxy-coumarin isolated from Ruta graveolens L. European Journal of Pharmacology. 2007;560:69-80.

43

43 Wadleigh DJ, Reddy ST, Kopp E, Ghosh S, Herschman HR. Transcriptional activation of the cyclooxygenase-2 gene in endotoxin-treated RAW 264.7 macrophages. J. Biol, Chem. 2000;275; 6259-6266.

44

44 Fiebich BL, Mueksch B, Boehringer M, Hull M. Interleukin-1$\beta$ induces cyclooxygenase-2 and prostaglandin D(2) synthesis in human neuroblastoma cells: involvement of p38 mitogenactivated protein kinase and nuclear factor-$\kappa$B. J. Neurochem. 2000;75:2020-2028.

45

45 Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SS, Kim YS. Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-$\kappa$B activation in RAW 264.7 cells. Life Sci. 2005;76:2315-28.

46

46 So HS, Park RK, Oh HM, Pae HO, Lee JH, Chai KY, Ahung SY, Chung HT. The methanol extract of Spiraea prunifolia var. simpliciflora root inhibits the generation of nitric oxide and superoxide in RAW 264.7 cells. J. Ethmopharmacol. 1999;68:209-17.

47

47 Tak PP, Firestein GS. NF-kappaB: a key role in inflammatory diseases. J Clin Invest. 2001;107: 7-11.

48

48 Caldenhoven E, Liden J, Wissink S, Stolpe AV, Raaijmakers H, Koenderman L, et al. Negative cross-talk between RelA and the glucocorticoid receptor, a possible mechanism for the antiinflammatory action of glucocorticoids. Mol. Endocrinol. 1995;9:401-412.

49

49 Grell M, Zimmermann G, Hulser D, Pfizenmaier K, Scheurich P. TNF receptor TR 60 and TR 80 can mediate apoptosis via induction of distinct signal pathway. J. Immunol. 1994;153:1963-1972.

50

50 Schreck R, Albermann K, Baeuerle PA. Nuclear factor kappa B, an oxidative stress responsive transcription factor of eukaryotic cells (a review), Free Radical Res. Comm. 1992;17:221-237.

51

51 Gospodarowicz D, Jones KL, Sato G. Purification of a growth factor for ovarian cell from bovine pituitary glands. Proc Natl Acad Sci USA. 1974;71:5364-8.

52

52 Bohlen P, Baird A, Esch F, Ling N, Gospodarowicz D. Isolation and partial molecular characterization of pituitary fibroblast growth factor. Proc Natl Acad Sci USA. 1984;81:5364-8.

53

53 Basilico C, Moscatelli D. The FGF family of growth factors and oncogenes. Adv. Cancer Res. 1992;59:115-165.

54

54 Burgess WH, Maciag T. The heparin binding(fibroblast) growth factor family of proteins. Annu. Rev. Biochem. 1989;58:575-606.

55

55 Galzie Z, Kinsella AR, Smith JA. Fibroblast grow factors and their receptors. Biochem, Cell Biol. 1997;75:669.

56

56 Fu X, Shen Z, Chen Y, Xie J, Guo Z, Zhang M, et al. Randomized placebo-controlled trial of use of topical recombinant bovine basic fibroblast growth factor for second degree burns. Lancet. 1998;352:1661-4.

57

57 Muneuchi G, Suzuki S, Moriue T, Igawa HH. Combined treatment using artificial dermis and basic fibroblast growth factor (bFGF) for intractable fingertip ulcers caused by atypical burn injuries. Burns. 2005;31:514-517.

58

58 Gratzner HG. Monoclonal antibody to 5-Bromoand 5-lododeoxyuridine. A new reagent for detection of DNA replication. Science. 1982;218: 474-5.

59

59 Lee JK, Kim JH, Nam KT, Lee SH. Molecular events associated with apoptosis and proliferation induced by ultraviolet-B radiation in the skin of hairless mice. J. Dermatological science. 2003; 32:171-179.

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