Article Detail

Home > Article Detail
  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

Anti-inflammatory Effects of Sam-chul-kun-bi-tang

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2010, v.31 no.3, pp.47-54
Jin-Ah Lee (Korea Institute of Oriental Medicine)
Hyekyung Ha (Korea Institute of Oriental Medicine)
Da-Young Jung (Korea Institute of Oriental Medicine,)
Ho-Young Lee (Korea Institute of Oriental Medicine)
Nam-Hun Lee (Korea Institute of Oriental Medicine)
Jun-Kyoung Lee (Korea Institute of Oriental Medicine)
Dae-Sun Huang (Korea Institute of Oriental Medicine)

  • Downloaded
  • Viewed

Abstract

Objective: To derive information on the efficacy of Sam-chul-kun-bi-tang (SKT), by evaluating its anti-inflammatory effect. SKT is a widely-used herbal formula in traditional Korean medicine. In many studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SKT extract on LPS-stimulated RAW 264.7 cells. Methods: The production of nitric oxide (NO), prostaglandin (PG)E2, tumor necrosis factor (TNF)-α and interleukin (IL)-6 were examined in a macrophage cell line, RAW 264.7 cells, in the presence of SKT. RAW 264.7 cells were incubated with LPS 1 μg/mL and SKT for 18 hrs. The anti-inflammatory activity of SKT was investigated by carrageenan-induced paw edema in rats. The paw volume was measured at 2 and 4 hrs following carrageenan-induced paw edema in rats. Results: SKT showed inhibitory effect on PGE2, TNF-α and IL-6 in LPS-stimulated RAW 264.7 cells. But SKT was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. Administration of SKT (1 g/kg) also showed a reduction in carrageenan-induced paw edema on rats. Conclusion: These results suggest that SKT has anti-inflammatory activities in both in vitro and in vivo models.

keywords
Anti-inflammation, Sam-chul-kun-bi-tang, herbal formula, lipopolysaccharide (LPS)


Reference

1

1 Lee SY, Kim JH. Effects of Samchulkunbitang on the immunosuppression induced by methotrexate in rats. J Korean oriental pediatrics 1998; 12: 257-276.

2

2 Kim SW, Lee TH. Effect of Samchulkunbitang on the gastric secretion and intestinal transport in the rat. J Korean oriental medical pathology 1988; 3:84-90.

3

3 Radad K, Gille G, Liu L, Rausch WD. Use of ginseng in medicine with emphasis on neurodegenerative disorders. J Pharmacol Sci 2006; 100: 175-186.

4

4 Park JS, Park EM, Kim DH, Jung K, Jung JS, Lee EJ et al. Anti-inflammatory mechanism of ginseng saponins in activated microglia. J Neuroimmunol 2009;209:40-49.

5

5 Chun HJ, Choi EY, Yoon SC, Nam HW, Baek SH, Woo WH. Inhibitory effects of ethanol extract of Atractylodis rhizome alba on melanin biosynthesis. The Pharmaceutical society of Korea 2001; 45:269-275.

6

6 Philip M, Rowley DA, Schreiber H. Inflammation as a tumor promoter in cancer induction. Semin Cancer Biol 2004; 14:433-439

7

7 Walsh LJ. Mast cells and oral inflammation. Crit Rev Oral Biol Med 2003; 14:188-198.

8

8 Ohshima H. Bartsch H. Chronic infections and inflammatory processes as cancer risk factors: possible role of nitric oxide in carcinogenesis. Mutat Res 1994; 305:253-264.

9

9 Winter CA, Risley EA, Nuss GW. Carrageenaninduced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962; 111:544-547.

10

10 Shin BG., Bailk WS, eds. Dongeuibogam. Seoul: Nam San Dang, 1983.

11

11 Hu XD, Yang Y, Zhong XG, Zhang XH, Zhang YN, Zheng ZP et al. Anti-inflammatory effects of Z23 on LPS-induced inflammatory responses in RAW 264.7 macrophages. J Ethnopharmacol 2008; 120:447-451.

12

12 Erridge C, Benett-Guerrero E, Poxton IR. Structure and function of lipopolysaccharides. Microbes Infect 2002; 4:837-851.

13

13 Hewett JA, Roth RA. Hepatic and extrahepatic pathobiology of bacterial lipopolysaccharides. Pharmacol Rev 1993; 45:382-411.

14

14 Palmer RM, Eveson JW. Chronic sialadenitis. An immunocytochemical study in humans. Virchows Arch A Pathol Anat Histopathol 1987; 412:73-78.

15

15 Szabó C, Ohshima H. DNA damage induced by peroxynitrite: subsequent biological effects. Nitric Oxide 1997; 1:373-385.

16

16 Vane JR. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat New Biol 1971; 231:232-235.

17

17 Van Snick J. Interleukin-6: an overview. Annu Rev Immunol 1990; 8:253-278.

18

18 Willeaume V, Kruys V, Mijatovic T, Huex G.. Tumor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. J Inflamm 1995-1996; 46:1-12.

19

19 Aukrust P, Ueland T, Lien E, Bendtzen K, Müller F, Andreassen AK et al. Cytokine network in congestive heart failure secondary to ischemic or idiopathic dilated cardiomyopathy. Am J Cardiol 1999; 83:376-382.

20

20 Rice TW, Bernard GR. Therapeutic intervention and targets for sepsis. Annu Rev Med 2005; 56:225-248.

21

21 Kim SJ, Jeong HJ, Moon PD, Lee KM, Lee HB, Jung HJ et al. Anti-inflammatory activity of gumiganghwaltang through the inhibition of nuclear factor-kappa B activation in peritoneal macrophages. Biol Pharm Bull 2005; 28:233-237.

22

22 Guay J, Bateman K, Gordon R Mancini J, Riendeau D. Carrageenan-induced paw edema in rat elicits a predominant prostaglandin $E_{2}$ (PG$E_{2}$) response in the central nervous system associated with the induction of microsomal PG$E_{2}$ synthase-1. J Biol Chem 2004; 279:24866-24872.

23

23 Gambero A, Becker TL, Gurgueira SA, Benvengo YH, Ribeiro ML, de Mendonça S et al. Acute inflammatory response induced by Helicobacter pylori in the rat air pouch. FEMS Immunol Med. Microbiol 2003; 38:193-198.

상단으로 이동

Journal of Korean Medicine