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  • P-ISSN 1010-0695
  • E-ISSN 2288-3339

The effects of Eclipta Prostrata L.(Ecliptae Herba) on periodontitis rats

Journal of Korean Medicine / Journal of Korean Medicine, (P)1010-0695; (E)2288-3339
2018, v.39 no.1, pp.63-74
https://doi.org/10.13048/jkm.18007



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Abstract

Objectives: Periodontitis is an inflammatory disease with the destruction of periodontal ligament, alveolar bone loss and inflammation of gingva, leading to teeth loss. Eclipta prostrata L. (Ecliptae Herba) has been used to treat the inflammatory disease as a Korean traditional medicine. The aim of this study is to investigate the effects of E. prostrata L. on periodontitis. Methods: E. prostrata L. was extracted with water and lyophilized. The aqueous extract of E. prostrata L. (EP) was topically applied to the periodontal lesion for 2 weeks. To induce the periodontitis, a 3-0 nylon ligature was placed around the cervix of the lower first molar in rat. Rats were divided into 3 groups (n = 7); NL group (non-ligatured and non-treated), L group (ligatured and vehicle-treated) and EP group (ligatured and EP-treated). After sacrifice, the mandibles was dissected and stained with methylene blue solution to analyze the alveolar bone loss. The expression of MMP-9 was determined in gingival tissues. To confirm the effect of EP on recovery of gingiva, mRNA expressions of type I pro-collagen and MMP-9 levels were investigated in LPS-treated HS68 fibroblast cells. In addition, inflammatory mediators were evaluated in LPS-treated RAW264.7 cells. Results: Alveolar bone loss was significantly inhibited by EP treatment. The mRNA expression of MMP-9 was attenuated in rats treated with EP. In addition, treatment with EP increased the expression of type I pro-collagen, while the expression of MMP-9 was decreased in LPS-stimulated HS68 fibroblast cells. Furthermore, EP down-regulated the LPS-induced IL-6, TNF-α, COX-2 and iNOS production in RAW264.7 cells. Conclusions: These results suggest that EP have ameliorative effects on periodontitis through inhibiting alveolar bone loss and modulating the inflammatory mediators. Therefore, E. prostrata L. may be an alternative on patients with periodontitis.

keywords
Eclipta prostrata L., periodontitis, MMP-9, collagen, inflammation


Reference

1

Petersen PE, Ogawa H. The global burden of periodontal disease: towards integration with chronic disease prevention and control. Periodontology 2000. 2012;60(1):15-39.

2

Renvert S, Persson GR. A systematic review on the use of residual probing depth, bleeding on probing and furcation status following initial periodontal therapy to predict further attachment and tooth loss. Journal of clinical periodontology. 2002;29(3):82-89.

3

Korea National Health and Nutrition Examination Survey. Ministry of Health, Welfare and Family Affairs. 2015.

4

Bartold PM, Van Dyke TE. Periodontitis: a host-mediated disruption of microbial homeostasis. Unlearning learned concepts. Periodontology 2000. 2013;62(1):203-217.

5

Socransky SS, Haffajee AD. Evidence of bacterial etiology: a historical perspective. Periodontology 2000. 1994;5(1):7-25.

6

Boyce BE, Li P, Yao Z, Zhang Q, Badell IR, Schwarz EM, et al. TNF-<TEX>${\alpha}$</TEX> and pathologic bone resorption. The Keio journal of medicine. 2005;54(3):127-131.

7

Apatzidou DA, Kinane DF. Nonsurgical mechanical treatment strategies for periodontal disease. Dental Clinics of North America. 2010;54(1):1-12.

8

Winkelhoff AJV, Rams TE, Slots J. Systemic antibiotic therapy in periodontics. Periodontology 2000. 1996;10(1):45-78.

9

Quirynen M, Teughels W, Soete MD, Steenberghe DV. Topical antiseptics and antibiotics in the initial therapy of chronic adult periodontitis: microbiological aspects. Periodontology 2000. 2002;28(1):72-90.

10

Kapoor A, Malhotra R, Grover V, Grover D. Systemic antibiotic therapy in periodontics. Dental research journal. 2012;9(5):505.

11

Heo J. Donguibogam. 1st ed. Seoul:Bubinmunhwasa. 1999:617-632.

12

Editing commission of herbal medicine. Herbal medicine. 1st ed. Seoul:Youngrimsa. 2010:658.

13

Yuan F, Chen J, Sun PP, Guan S, Xu J. Wedelolactone inhibits LPS-induced pro-inflammation via NF-<TEX>${\kappa}B$</TEX> pathway in RAW264.7 cells. Journal of biomedical science. 2013;20(1):84.

14

Arunachalam, G, Subramanian N, Pazhani GP, Ravichandran V. Anti-inflammatory activity of methanolic extract of Eclipta prostrata L (Astearaceae). African journal of pharmacy and pharmacology. 2009;3(3):097-100.

15

Tewtrakul S, Subhadhirasakul S, Tansakul P, Cheenpracha S, Karalai C. Antiinflammatory constituents from Eclipta prostrata using RAW 264. 7 macrophage cells. Phytotherapy research. 2011;25(9):1313-1316.

16

Kim MH, Choi YY, Lee HJ, Lee HS, Park JC, Yang WM. Topical application of herbal formula for the treatment of ligature-induced periodontitis. Journal of periodontal & implant science. 2015;45(4):145-151.

17

Lohinai Z, Benedek P, Feher E, Gyor A, Rosival F, Fazeka IA, et al. Protective effects of mercaptoethylguanidine, a selective inhibitor of inducible nitric oxide synthase, in ligature-induced periodontitis in the rat. British journal of pharmacology. 1998;123(3):353-360.

18

Mine T, Wactawski-Wende J, Grossi SG, Ho AW, Dunford R, Genco RJ. The relationship between bone mineral density and periodontitis in postmenopausal women. Journal of Periodontology. 2000;71(9):1492-1498.

19

Makela M, Salo T, Uitto VJ, Larjava H. Matrix metalloproteinases (MMP-2 and MMP-9) of the oral cavity: cellular origin and relationship to periodontal status. Journal of dental research. 1994;73(8):1397-1406.

20

Marcaccini AM, Novaes Jr AB, Meschiari CA, Souza SL, Palioto DB, Sorgi CA, et al. Circulating matrix metalloproteinase-8 (MMP-8) and MMP-9 are increased in chronic periodontal disease and decrease after non-surgical periodontal therapy. Clinica Chimica Acta. 2009;409(1):117-122.

21

Elavarasu S, Sekar S, Murugan T. Host modulation by therapeutic agents. Journal of pharmacy & bioallied sciences. 2012;4(Suppl 2):S256.

22

Choi DH, Moon IS, Choi BK, Paik JW, Kim YS, Choi SH, et al. Effects of sub-antimicrobial dose doxycycline therapy on crevicular fluid MMP-8, and gingival tissue MMP-9, TIMP-1 and IL-6 levels in chronic periodontitis. Journal of periodontal research. 2004;39(1):20-26.

23

Paul B, Sage H. Structurally distinct collagen types. Annual review of biochemistry. 1980;49(1):957-1003.

24

Bumann A, Carvalho RS, Schwarzer CL, Yen EHK. Collagen synthesis from human PDL cells following orthodontic tooth movement. European journal of orthodontics. 1997;19(1):29-37.

25

Bohl MV, Maltha J, Hoff HVD, Muijpers-jagtman AM. Changes in the periodontal ligament after experimental tooth movement using high and low continuous forces in beagle dogs. The Angle orthodontist. 2004.74(1):16-25.

26

Havemose-Poulsen A, Holmstrup P, Stoltze K, Birkedal-Hansen H. Dissolution of type I collagen fibrils by gingival fibroblasts isolated from patients of various periodontitis categories. Journal of periodontal research. 1998;33(3):280-291.

27

Okada H, Murakami S. Cytokine expression in periodontal health and disease. Critical Reviews in Oral Biology & Medicine. 1998;9(3):248-266.

28

Nibali L, Fedele S, Daiuto F, Donos N. Interleukin-6 in oral diseases: a review. Oral diseases. 2012;18(3):236-243.

29

Matsuda Y, Katoo T, Takahashi N. Ligature-induced periodontitis in mice induces elevated levels of circulating interleukin-6 but shows only weak effects on adipose and liver tissues. Journal of periodontal research. 2016;51(5):639-646.

30

Geivelis M, Turner DW, Pederson ED, Lamberts BL. Measurements of interleukin-6 in gingival crevicular fluid from adults with destructive periodontal disease. Journal of periodontology. 1993;64(10):980-983.

31

Rossomando EF, Kennedy JE, Hadjimichael J. Tumour necrosis factor alpha in gingival crevicular fluid as a possible indicator of periodontal disease in humans. Archives of oral biology. 1990;35(6):431-434.

32

Boyce BE, Li P, Yao Z, Zhang Q, Badell IR, Schwarz EM, et al. TNF-<TEX>${\alpha}$</TEX> and pathologic bone resorption. The Keio journal of medicine. 2005;54(3):127-131.

33

Lawrence T, Gilroy DW, Colville-Nash PR, Willoughby DA. Possible new role for NF-<TEX>${\kappa}B$</TEX> in the resolution of inflammation. Nature medicine. 2001;7(12):1291-1297.

34

Moncada S, Palmer RML, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacological reviews. 1991;43(2):109-142.

35

MacMicking J, Xie Q, Nathan C. Nitric oxide and macrophage function. Annual review of immunology. 1997;15(1):323-350.

36

Tunctan B, Uluda O, Altu S, Abacio N. Effects of nitric oxide synthase inhibition in lipopolysaccharide-induced sepsis in mice. Pharmacological research. 1998;38(5):405-411.

37

Daghigh F, Borghaei RC, Thornton RD, Bee JH. Human gingival fibroblasts produce nitric oxide in response to proinflammatory cytokines. Journal of periodontology. 2002;73(4):392-400.

38

Lohinai Z, Stachlewitz R, Virag L, Szekely AD, Hasko G, Szabo C. Evidence for reactive nitrogen species formation in the gingivomucosal tissue. Journal of dental research. 2001;80(2):470-475.

39

Morton RS, Dongari-Bagtzoglou AI. Cyclooxygenase-2 is upregulated in inflamed gingival tissues. Journal of periodontology. 2001;72(4):461-469.

40

Holzhausen M, Rossa Jr C, Marcantonio Jr E, Nassar PO, Spolidorio DMP, Spolidorio LC. Effect of selective cyclooxygenase-2 inhibition on the development of ligature-induced periodontitis in rats. Journal of periodontology. 2002;73(9):1030-1036.

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