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  • P-ISSN2765-2203
  • E-ISSN2765-2211
  • KCI Candidate

Species Identification Method via Unique Genetic Markers for Reticulitermes kanmonensis

Proceedings of the National Institute of Ecology of the Republic of Korea / Proceedings of the National Institute of Ecology of the Republic of Korea, (P)2765-2203; (E)2765-2211
2024, v.5 no.4, pp.128-133
https://doi.org/10.22920/PNIE.2024.5.4.128
Soon Jae Eum (Invasive Alien Species Team, National Institute of Ecology)
Kibeom Park (Invirustech)
Youngho Cho (Invasive Alien Species Team, National Institute of Ecology)
Youngjun Park (Invasive Alien Species Team, National Institute of Ecology)

Abstract

Invasive alien species, along with climate change, are major contributing factors to biodiversity loss, and their unintentional introduction through imported goods is increasing. Termites, as wood-feeding insects, pose a significant threat when introduced into Korea via imported wood, necessitating a rapid and accurate identification method. In this study, we developed a diagnostic method based on species-specific genetic sequences for identifying Reticulitermes kanmonensis, a recently identified species. Termite samples were collected from Wanju-gun, Jeollabuk-do, and subjected to whole-genome sequencing to pinpoint species-specific genetic sequences. Utilizing these sequences, we designed primer sets and employed TaqMan-based primer sets and qPCR analysis to select the final primer sets capable of rapidly distinguishing R. kanmonensis. The genetic detection method developed here offers a rapid means of identifying alien termite species, likely enhancing termite management and quarantine practices in Korea.

keywords
Specific markers, Termites, Reticulitermes kanmonensis
Submission Date
2024-09-23
Revised Date
2024-10-18
Accepted Date
2024-10-19

Proceedings of the National Institute of Ecology of the Republic of Korea