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Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy againstMycobacterium tuberculosis Infection in a Mouse Model

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2004, v.57 no.2, pp.125-131







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Abstract

Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over- expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model. (Tuberc Respir Dis 2004; 57:125-131)

keywords
Tuberculosis, Vaccine, Recombinant BCG., Tuberculosis, Vaccine, Recombinant BCG.

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Tuberculosis & Respiratory Diseases