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ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

항원 85 복합체를 과발현하는 재조합 BCG의 개발 및마우스 모델에 있어서의 결핵균 감염에 대한 방어 효능

Construction of Recombinant BCGs Overexpressing Antigen 85 Complex and Their Protective Efficacy againstMycobacterium tuberculosis Infection in a Mouse Model

Tuberculosis & Respiratory Diseases / Tuberculosis & Respiratory Diseases,
2004, v.57 no.2, pp.125-131
이승헌 (대한결핵협회)
전보영 (연세대학교)
이혜영 (연세대학교)
조상래 (연세대학교)
배길한 (대한결핵협회 결핵연구원)
박영길 (대한결핵협회 결핵연구원)
김효준 (한양대학교)
  • 다운로드 수
  • 조회수

Abstract

Tuberculosis (TB) remains an enormous global health problem, and a new vaccine against TB more potent than the current inadequate BCG vaccine is urgently needed. We constructed three recombinant Mycobacterium bovis BCG (rBCG) strains over-expressing antigen (Ag) 85A, Ag85B, or both of M. tuberculosis using their own promoter and secretory sequence, or hsp60 promoter. SDS-PAGE analysis of rBCG proteins showed over- expression of Ag85A and Ag85B proteins in higher level than of those in their parental strain of BCG. In addition, rBCG(rBCG/B.FA) over-expressing Ag85A and Ag85B induced strong IFN- production in splenocytes. However, there was no significant difference in protective efficacy between rBCG and their parental BCG strain. In this study, therefore, rBCG over-expressing Ag85A, Ag85B, or both failed to show enhanced protection against M. tuberculosis infection in a mouse model. (Tuberc Respir Dis 2004; 57:125-131)

keywords
Tuberculosis, Vaccine, Recombinant BCG., Tuberculosis, Vaccine, Recombinant BCG.

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Tuberculosis & Respiratory Diseases